Abstract
The aim of creating induced mutations is to bring about genetic changes that increase the variation in the desired properties. The selection of appropriate mutagens depends on their availability and effectiveness at certain doses to achieve the necessary genetic modifications. This research was performed at the Dicle University Faculty of Agriculture Biotechnology Laboratory to determine the effects of 10 specific doses of EMS (Ethyl Methane Sulfonate) on mutation on the chickpea cv. Gökçe of germination characters. Seed was handled with an EMS of 0 (control), 10, 20, 30, 40, 50, 60, 70, 80, 90, 100 mM. In the germination cabinet, EMS applied seeds were left to germinate with 3 replications according to the completely randomized design with 25 seeds in each petri dishes. In the study, germination rate, coleoptile length, root length, coleoptile fresh weight, root fresh weight, coleoptile dry weight and root dry weight were measured. It was decided that there was a significant difference in all the germination features tested between the doses applied. Due to the rise in EMS doses, a reduction was defined for all features and the lowest values were obtained from the dosage of 100 mM. As a result of the study, it was determined that increasing the dose of EMS in chickpea reduces the germination rate, the seedling characteristics and the healthy growth rate of the seeds and that the effective mutation can be obtained from 50-60 mM doses.
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Claims 6
Claim · model-written summary
EMS doses differed significantly for all tested germination features.
- Taxon
- Chickpea
Cicer arietinumt0 - Treatment
- doses appliede0
- Outcome
- all the germination features testede0
- Comparator
- between the doses appliede0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there was a significant difference in all the germination features tested between the doses applied”
t0“Chickpea (Cicer arietinum L.)”
Claim · model-written summary
Rising EMS doses reduced germination rate.
- Taxon
- chickpeaCicer arietinume0
- Treatment
- increasing the dose of EMSe0
- Outcome
- germination ratee0
- Direction
- reducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increasing the dose of EMS in chickpea reduces the germination rate”
t0“Chickpea (Cicer arietinum L.)”
Claim · model-written summary
Rising EMS doses reduced seedling characteristics.
- Taxon
- chickpeaCicer arietinume0
- Treatment
- increasing the dose of EMSe0
- Outcome
- seedling characteristicse0
- Direction
- reducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increasing the dose of EMS in chickpea reduces the germination rate, the seedling characteristics and the healthy growth rate of the seeds”
t0“Chickpea (Cicer arietinum L.)”
Claim · model-written summary
Rising EMS doses reduced healthy seed growth rate.
- Taxon
- chickpeaCicer arietinume0
- Treatment
- increasing the dose of EMSe0
- Outcome
- healthy growth rate of the seedse0
- Direction
- reducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increasing the dose of EMS in chickpea reduces the germination rate, the seedling characteristics and the healthy growth rate of the seeds”
t0“Chickpea (Cicer arietinum L.)”
Claim · model-written summary
The lowest germination and seedling trait values occurred at 100 mM EMS.
- Taxon
- ChickpeaCicer arietinumt0
- Treatment
- dosage of 100 mMe0
- Outcome
- lowest valuese0
- Direction
- loweste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the lowest values were obtained from the dosage of 100 mM”
t0“Chickpea (Cicer arietinum L.)”
Claim · model-written summary
The authors concluded that 50-60 mM EMS doses can yield effective mutation.
- Taxon
- ChickpeaCicer arietinumt0
- Treatment
- 50-60 mM dosese0
- Outcome
- effective mutatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“effective mutation can be obtained from 50-60 mM doses”
t0“Chickpea (Cicer arietinum L.)”
The same questions in other papers
ethyl methanesulfonate · growth · Cicer arietinum (chickpea)Results differ
6 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- negative
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.