Abstract
Arabidopsis SYSTEMIC ACQUIRED RESISTANCE DEFICIENT 1 (SARD1) and CALMODULIN-BINDING PROTEIN 60g (CBP60g) are two master transcription factors that regulate many defense-related genes in plant immunity. They are required for immunity downstream of the receptor-like protein SUPPRESSOR OF NPR1-1, CONSTITUTIVE 2 (SNC2). Constitutive defense responses in the gain-of-function autoimmune snc2-1D mutant are modestly affected in either sard1 or cbp60g single mutants but completely suppressed in the sard1 cbp60g double mutant. Here we report that CBP60b, another member of the CBP60 family, also functions as a positive regulator of SNC2-mediated immunity. Loss-of-function mutations of CBP60b suppress the constitutive expression of SARD1 and enhanced disease resistance in cbp60g-1 snc2-1D, whereas overexpression of CBP60b leads to elevated SARD1 expression and constitutive defense responses. In addition, transient expression of CBP60b in Nicotiana benthamiana activates the expression of the pSARD1::luciferase reporter gene. Chromatin immunoprecipitation assays further showed that CBP60b is recruited to the promoter region of SARD1, suggesting that it directly regulates SARD1 expression. Interestingly, knocking out CBP60b in the wild-type background leads to ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1)-dependent autoimmunity, suggesting that CBP60b is required for the expression of a guardee/decoy or a negative regulator of immunity mediated by receptors carrying an N-terminal Toll-interleukin-1 receptor-like domain.
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Claims 9
Claim · model-written summary
CBP60b functions as a positive regulator of SNC2-mediated immunity.
- Taxon
- Arabidopsis
Arabidopsist0 - Gene
- CBP60be1
- Outcome
- SNC2-mediated immunitye1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis CALMODULIN-BINDING PROTEIN 60b”
e1“CBP60b, another member of the CBP60 family, also functions as a positive regulator of SNC2-mediated immunity”
Claim · model-written summary
Loss-of-function mutations of CBP60b suppress constitutive SARD1 expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Loss-of-function mutations of CBP60be2
- Gene
- SARD1e2
- Outcome
- constitutive expression of SARD1e2
- Direction
- suppresse2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis CALMODULIN-BINDING PROTEIN 60b”
e2“Loss-of-function mutations of CBP60b suppress the constitutive expression of SARD1 and enhanced disease resistance in cbp60g-1 snc2-1D”
Claim · model-written summary
Loss-of-function mutations of CBP60b suppress enhanced disease resistance in cbp60g-1 snc2-1D.
- Taxon
- ArabidopsisArabidopsist0
- Material
- cbp60g-1 snc2-1De2
- Treatment
- Loss-of-function mutations of CBP60be2
- Outcome
- enhanced disease resistancee2
- Direction
- suppresse2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis CALMODULIN-BINDING PROTEIN 60b”
e2“Loss-of-function mutations of CBP60b suppress the constitutive expression of SARD1 and enhanced disease resistance in cbp60g-1 snc2-1D”
Claim · model-written summary
Overexpression of CBP60b elevates SARD1 expression and constitutive defense responses.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- overexpression of CBP60be3
- Gene
- SARD1e3
- Outcome
- SARD1 expression and constitutive defense responsese3
- Direction
- elevatede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis CALMODULIN-BINDING PROTEIN 60b”
e3“overexpression of CBP60b leads to elevated SARD1 expression and constitutive defense responses”
Claim · model-written summary
Transient expression of CBP60b in Nicotiana benthamiana activates the pSARD1::luciferase reporter gene.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae4 - Treatment
- transient expression of CBP60be4
- Outcome
- expression of the pSARD1::luciferase reporter genee4
- Direction
- activatese4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“transient expression of CBP60b in Nicotiana benthamiana activates the expression of the pSARD1::luciferase reporter gene”
Claim · model-written summary
CBP60b is recruited to the promoter region of SARD1.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- SARD1e5
- Outcome
- recruited to the promoter region of SARD1e5
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis CALMODULIN-BINDING PROTEIN 60b”
e5“CBP60b is recruited to the promoter region of SARD1”
Claim · model-written summary
CBP60b is suggested to directly regulate SARD1 expression.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- SARD1e6
- Outcome
- SARD1 expressione6
- Qualifier
- directly regulatese6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis CALMODULIN-BINDING PROTEIN 60b”
e6“suggesting that it directly regulates SARD1 expression”
Claim · model-written summary
Knocking out CBP60b in the wild-type background leads to EDS1-dependent autoimmunity.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- knocking out CBP60be7
- Gene
- EDS1e7
- Outcome
- autoimmunitye7
- Direction
- leads toe7
- Comparator
- wild-type backgrounde7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis CALMODULIN-BINDING PROTEIN 60b”
e7“knocking out CBP60b in the wild-type background leads to ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1)-dependent autoimmunity”
Claim · model-written summary
CBP60b is suggested to be required for expression of a guardee/decoy or a negative regulator of immunity.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- expression of a guardee/decoy or a negative regulator of immunitye1
- Qualifier
- required for the expressione1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis CALMODULIN-BINDING PROTEIN 60b”
e1“suggesting that CBP60b is required for the expression of a guardee/decoy or a negative regulator of immunity”
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See all 147 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.