Abstract
Harsh environmental factors have continuous negative effects on plant growth and development, leading to metabolic disruption and reduced plant productivity and quality. However, filamentation temperature-sensitive H protease (FtsH) plays a prominent role in helping plants to cope with these negative impacts. In the current study, we examined the transcriptional regulation of the CaFtsH06 gene in the R9 thermo-tolerant pepper (Capsicum annuum L.) line. The results of qRT-PCR revealed that CaFtsH06 expression was rapidly induced by abiotic stress treatments, including heat, salt, and drought. The CaFtsH06 protein was localized to the mitochondria and cell membrane. Additionally, silencing CaFtsH06 increased the accumulation of malonaldehyde content, conductivity, hydrogen peroxide (H2O2) content, and the activity levels of superoxide dismutase and superoxide (·O2−), while total chlorophyll content decreased under these abiotic stresses. Furthermore, CaFtsH06 ectopic expression enhanced tolerance to heat, salt, and drought stresses, thus decreasing malondialdehyde, proline, H2O2, and ·O2− contents while superoxide dismutase activity and total chlorophyll content were increased in transgenic Arabidopsis. Similarly, the expression levels of other defense-related genes were much higher in the transgenic ectopic expression lines than WT plants. These results suggest that CaFtsH06 confers abiotic stress tolerance in peppers by interfering with the physiological indices through reducing the accumulation of reactive oxygen species, inducing the activities of stress-related enzymes and regulating the transcription of defense-related genes, among other mechanisms. The results of this study suggest that CaFtsH06 plays a very crucial role in the defense mechanisms of pepper plants to unfavorable environmental conditions and its regulatory network with other CaFtsH genes should be examined across variable environments.
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Claims 10
Claim · model-written summary
Heat, salt and drought rapidly induced CaFtsH06 expression in pepper.
- Taxon
- peppert0
- Treatment
- abiotic stress treatments, including heat, salt, and droughte0
- Gene
- CaFtsH06e0
- Outcome
- CaFtsH06 expressione0
- Direction
- rapidly inducede0
Not reportedComparator
Verbatim
e0“CaFtsH06 expression was rapidly induced by abiotic stress treatments, including heat, salt, and drought.”
t0“pepper (Capsicum annuum L.)”
Claim · model-written summary
CaFtsH06 protein localized to the mitochondria and cell membrane.
Verbatim
e0“The CaFtsH06 protein was localized to the mitochondria and cell membrane.”
t0“pepper (Capsicum annuum L.)”
Claim · model-written summary
CaFtsH06 silencing increased malonaldehyde content, conductivity and hydrogen peroxide content under abiotic stresses.
- Taxon
- peppert0
- Treatment
- silencing CaFtsH06e0
- Gene
- CaFtsH06e0
- Outcome
- malonaldehyde content, conductivity, hydrogen peroxide (H2O2) contente0
- Direction
- increasede0
- Qualifier
- under these abiotic stressese0
Not reportedComparator
Verbatim
e0“silencing CaFtsH06 increased the accumulation of malonaldehyde content, conductivity, hydrogen peroxide (H2O2) content, and the activity levels of superoxide dismutase and superoxide (·O2−), while total chlorophyll content decreased under these abiotic stresses.”
t0“pepper (Capsicum annuum L.)”
Claim · model-written summary
CaFtsH06 silencing increased superoxide dismutase and superoxide activity levels under abiotic stresses.
- Taxon
- peppert0
- Treatment
- silencing CaFtsH06e0
- Gene
- CaFtsH06e0
- Outcome
- activity levels of superoxide dismutase and superoxide (·O2−)e0
- Direction
- increasede0
- Qualifier
- under these abiotic stressese0
Not reportedComparator
Verbatim
e0“silencing CaFtsH06 increased the accumulation of malonaldehyde content, conductivity, hydrogen peroxide (H2O2) content, and the activity levels of superoxide dismutase and superoxide (·O2−), while total chlorophyll content decreased under these abiotic stresses.”
t0“pepper (Capsicum annuum L.)”
Claim · model-written summary
CaFtsH06 silencing decreased total chlorophyll content under abiotic stresses.
- Taxon
- peppert0
- Treatment
- silencing CaFtsH06e1
- Gene
- CaFtsH06e1
- Outcome
- total chlorophyll contente0
- Direction
- decreasede0
Not reportedComparator
Verbatim
e0“while total chlorophyll content decreased under these abiotic stresses.”
e1“silencing CaFtsH06”
t0“pepper (Capsicum annuum L.)”
Claim · model-written summary
Ectopic CaFtsH06 expression enhanced heat, salt and drought tolerance in transgenic Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise1 - Treatment
- CaFtsH06 ectopic expressione0
- Gene
- CaFtsH06e0
- Outcome
- tolerance to heat, salt, and drought stressese0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CaFtsH06 ectopic expression enhanced tolerance to heat, salt, and drought stresses”
e1“in transgenic Arabidopsis.”
Claim · model-written summary
Ectopic CaFtsH06 expression decreased malondialdehyde, proline, H2O2 and superoxide contents in transgenic Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- CaFtsH06 ectopic expressione0
- Gene
- CaFtsH06e0
- Outcome
- malondialdehyde, proline, H2O2, and ·O2− contentse0
- Direction
- decreasinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CaFtsH06 ectopic expression enhanced tolerance to heat, salt, and drought stresses, thus decreasing malondialdehyde, proline, H2O2, and ·O2− contents while superoxide dismutase activity and total chlorophyll content were increased in transgenic Arabidopsis.”
Claim · model-written summary
Ectopic CaFtsH06 expression increased superoxide dismutase activity and total chlorophyll content in transgenic Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- CaFtsH06 ectopic expressione0
- Gene
- CaFtsH06e0
- Outcome
- superoxide dismutase activity and total chlorophyll contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CaFtsH06 ectopic expression enhanced tolerance to heat, salt, and drought stresses, thus decreasing malondialdehyde, proline, H2O2, and ·O2− contents while superoxide dismutase activity and total chlorophyll content were increased in transgenic Arabidopsis.”
Claim · model-written summary
Defense-related gene expression was much higher in transgenic ectopic expression lines than WT plants.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- transgenic ectopic expression linese0
- Gene
- defense-related genese0
- Outcome
- expression levels of other defense-related genese0
- Direction
- much highere0
- Comparator
- WT plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression levels of other defense-related genes were much higher in the transgenic ectopic expression lines than WT plants.”
e1“in transgenic Arabidopsis.”
Claim · model-written summary
The authors infer that CaFtsH06 confers abiotic stress tolerance in pepper through changes in physiological indices, reactive oxygen species, stress enzymes and defense gene transcription.
- Gene
- CaFtsH06e0
- Outcome
- abiotic stress tolerancee0
- Qualifier
- reducing the accumulation of reactive oxygen species, inducing the activities of stress-related enzymes and regulating the transcription of defense-related genese0
CapsicumOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CaFtsH06 confers abiotic stress tolerance in peppers by interfering with the physiological indices through reducing the accumulation of reactive oxygen species, inducing the activities of stress-related enzymes and regulating the transcription of defense-related genes”