Abstract
The brassinosteroid pathway promotes a variety of physiological processes in plants and the brassinosteroid insensitive1-ethylmethane sulfonate suppressor (BES)/brassinazole-resistant (BZR) functions as one of its key regulators. We previously showed that the BES/BZR-type transcription factor TaBZR2 mediates the drought stress response in wheat (Triticum aestivum) by directly upregulating the transcriptional activity of glutathione S-transferase 1. However, the function of TaBZR2 in plants under biotic stresses is unknown. In this study, we found that transcript levels of TaBZR2 were upregulated in response to inoculation with wheat stripe rust fungus (Puccinia striiformis f. sp. tritici, Pst) and treatment with flg22 or an elicitor-like protein of Pst, Pst322. Wheat lines overexpressing TaBZR2 conferred increased resistance, whereas TaBZR2-RNAi lines exhibited decreased resistance to multiple races of Pst. TaBZR2 targeted the promoter of the chitinase gene TaCht20.2, activating its transcription. Knockdown of TaCht20.2 in wheat resulted in enhanced susceptibility to Pst, indicating the positive role of TaCht20.2 in wheat resistance. Upon Pst infection in vivo, the overexpression of TaBZR2 increased total chitinase activity, whereas RNAi-mediated silencing of TaBZR2 reduced total chitinase activity. Taken together, our results suggest that TaBZR2 confers broad-spectrum resistance to the stripe rust fungus by increasing total chitinase activity in wheat.
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Claims 9
Claim · model-written summary
Pst inoculation upregulated TaBZR2 transcripts in wheat.
- Taxon
- wheat
Triticum aestivume0 - Treatment
- inoculation with wheat stripe rust funguse0
- Interactor
- Puccinia striiformis f. sp. tritici
Puccinia striiformis f. sp. triticie0pathogen - Gene
- TaBZR2e0
- Outcome
- transcript levels of TaBZR2e0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcript levels of TaBZR2 were upregulated in response to inoculation with wheat stripe rust fungus (Puccinia striiformis f. sp. tritici, Pst)”
Claim · model-written summary
Flg22 treatment upregulated TaBZR2 transcripts in wheat.
- Taxon
- wheatTriticum aestivume0
- Treatment
- treatment with flg22e0
- Gene
- TaBZR2e0
- Outcome
- transcript levels of TaBZR2e0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcript levels of TaBZR2 were upregulated in response to inoculation with wheat stripe rust fungus (Puccinia striiformis f. sp. tritici, Pst) and treatment with flg22 or an elicitor-like protein of Pst, Pst322”
Claim · model-written summary
Pst322 treatment upregulated TaBZR2 transcripts in wheat.
- Taxon
- wheatTriticum aestivume0
- Treatment
- Pst322e0
- Gene
- TaBZR2e0
- Outcome
- transcript levels of TaBZR2e0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcript levels of TaBZR2 were upregulated in response to inoculation with wheat stripe rust fungus (Puccinia striiformis f. sp. tritici, Pst) and treatment with flg22 or an elicitor-like protein of Pst, Pst322”
Claim · model-written summary
TaBZR2 overexpression increased wheat resistance to multiple Pst races.
- Taxon
- WheatTriticum aestivume0
- Treatment
- overexpressing TaBZR2e0
- Interactor
- Pste1pathogen
- Gene
- TaBZR2e0
- Outcome
- resistance to multiple races of Pste0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wheat lines overexpressing TaBZR2 conferred increased resistance, whereas TaBZR2-RNAi lines exhibited decreased resistance to multiple races of Pst”
e1“Puccinia striiformis f. sp. tritici, Pst”
Claim · model-written summary
TaBZR2 RNAi decreased wheat resistance to multiple Pst races.
- Taxon
- WheatTriticum aestivume0
- Treatment
- TaBZR2-RNAie0
- Interactor
- Pste1pathogen
- Gene
- TaBZR2e0
- Outcome
- resistance to multiple races of Pste0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Wheat lines overexpressing TaBZR2 conferred increased resistance, whereas TaBZR2-RNAi lines exhibited decreased resistance to multiple races of Pst”
e1“Puccinia striiformis f. sp. tritici, Pst”
Claim · model-written summary
TaBZR2 targeted the TaCht20.2 promoter and activated its transcription.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaBZR2e0
- Outcome
- TaCht20.2, activating its transcriptione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“wheat”
e0“TaBZR2 targeted the promoter of the chitinase gene TaCht20.2, activating its transcription”
Claim · model-written summary
TaCht20.2 knockdown enhanced wheat susceptibility to Pst.
- Taxon
- wheatTriticum aestivume0
- Treatment
- Knockdown of TaCht20.2e0
- Interactor
- Pste1pathogen
- Gene
- TaCht20.2e0
- Outcome
- susceptibility to Pste0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knockdown of TaCht20.2 in wheat resulted in enhanced susceptibility to Pst”
e1“Puccinia striiformis f. sp. tritici, Pst”
Claim · model-written summary
TaBZR2 overexpression increased total chitinase activity after Pst infection.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- overexpression of TaBZR2e0
- Interactor
- Pste1pathogen
- Gene
- TaBZR2e0
- Outcome
- total chitinase activitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Upon Pst infection in vivo, the overexpression of TaBZR2 increased total chitinase activity”
t0“wheat”
e1“Puccinia striiformis f. sp. tritici, Pst”
Claim · model-written summary
TaBZR2 silencing reduced total chitinase activity after Pst infection.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- RNAi-mediated silencing of TaBZR2e0
- Interactor
- Pste1pathogen
- Gene
- TaBZR2e0
- Outcome
- total chitinase activitye0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Upon Pst infection in vivo, the overexpression of TaBZR2 increased total chitinase activity, whereas RNAi-mediated silencing of TaBZR2 reduced total chitinase activity”
t0“wheat”
e1“Puccinia striiformis f. sp. tritici, Pst”
The same questions in other papers
loss of function · physiology: antioxidant enzymes · Triticum aestivum (wheat)Positive and negative results
1 in the opposite direction
- positive
inoculation · Puccinia striiformis f. sp. tritici · Triticum aestivum (wheat)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
resistance inducer · molecular: expression · Triticum aestivum (wheat)Results agree
5 papers in the same direction as this paper
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.