Abstract
Autophagy-related 8 (ATG8) protein has been reported to be involved in plant’s innate immune response, but it is not clear whether such genes play a similar role in cereal crops against obligate biotrophic fungal pathogens. Here, we reported an ATG8 gene from wheat (Triticum aestivum), designated TaATG8j. This gene has three copies located in chromosomes 2AS, 2BS, and 2DS. The transcriptions of all three copies were upregulated in plants of the wheat cultivar Suwon 11, inoculated with an avirulent race (CYR23) of Puccinia striiformis f. sp. tritici (Pst), the causal fungal pathogen of stripe rust. The transient expression of TaATG8j in Nicotiana benthamiana showed that TaATG8j proteins were distributed throughout the cytoplasm, but mainly in the nucleus and plasma membrane. The overexpression of TaATG8j in N. benthamiana slightly delayed the cell death caused by the mouse apoptotic protein BAX (BCL2-associated X protein). However, the expression of TaATG8j in yeast (Schizosaccharomyces pombe) induced cell death. The virus-induced gene silencing of all TaATG8j copies rendered Suwon 11 susceptible to the avirulent Pst race CYR23, accompanied by an increased fungal biomass and a decreased necrotic area per infection site. These results indicate that TaATG8j contributes to wheat resistance against stripe rust fungus by regulating cell death, providing information for the understanding of the mechanisms of wheat resistance to the stripe rust pathogen.
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Claims 8
Claim · model-written summary
TaATG8j has three chromosomal copies.
- Taxon
- wheat
Triticum aestivumt0 - Gene
- TaATG8jt0
- Outcome
- three copiese0
- Value
- three copiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This gene has three copies located in chromosomes 2AS, 2BS, and 2DS”
t0“Wheat Gene TaATG8j”
Claim · model-written summary
All three TaATG8j copies were upregulated following avirulent Pst inoculation in Suwon 11.
- Taxon
- wheatTriticum aestivume0
- Material
- Suwon 11e0
- Treatment
- inoculated with an avirulent race (CYR23)e0
- Interactor
- Puccinia striiformis f. sp. tritici
Puccinia striiformis f. sp. triticie0pathogen - Gene
- TaATG8jt0
- Outcome
- transcriptions of all three copiese0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcriptions of all three copies were upregulated in plants of the wheat cultivar Suwon 11, inoculated with an avirulent race (CYR23) of Puccinia striiformis f. sp. tritici (Pst)”
t0“Wheat Gene TaATG8j”
Claim · model-written summary
TaATG8j proteins localized throughout cytoplasm, chiefly nucleus and plasma membrane in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Gene
- TaATG8je0
- Outcome
- distributed throughout the cytoplasm, but mainly in the nucleus and plasma membranee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transient expression of TaATG8j in Nicotiana benthamiana showed that TaATG8j proteins were distributed throughout the cytoplasm, but mainly in the nucleus and plasma membrane”
Claim · model-written summary
TaATG8j overexpression slightly delayed BAX induced cell death in N. benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- overexpression of TaATG8je0
- Gene
- TaATG8je0
- Outcome
- cell deathe0
- Direction
- delayede0
- Qualifier
- slightlye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The overexpression of TaATG8j in N. benthamiana slightly delayed the cell death caused by the mouse apoptotic protein BAX”
Claim · model-written summary
TaATG8j expression induced yeast cell death.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- expression of TaATG8je0
- Gene
- TaATG8je0
- Outcome
- yeast (Schizosaccharomyces pombe) induced cell deathe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of TaATG8j in yeast (Schizosaccharomyces pombe) induced cell death”
t0“Wheat Gene TaATG8j”
Claim · model-written summary
Silencing all TaATG8j copies rendered Suwon 11 susceptible to avirulent Pst CYR23.
- Taxon
- wheatTriticum aestivumt0
- Material
- Suwon 11e0
- Treatment
- virus-induced gene silencing of all TaATG8j copiese0
- Interactor
- Pste0pathogen
- Gene
- TaATG8je0
- Outcome
- susceptible to the avirulent Pst race CYR23e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The virus-induced gene silencing of all TaATG8j copies rendered Suwon 11 susceptible to the avirulent Pst race CYR23”
t0“Wheat Gene TaATG8j”
Claim · model-written summary
Silencing TaATG8j increased fungal biomass.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- virus-induced gene silencing of all TaATG8j copiese0
- Interactor
- Pste0pathogen
- Gene
- TaATG8je0
- Outcome
- fungal biomasse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The virus-induced gene silencing of all TaATG8j copies rendered Suwon 11 susceptible to the avirulent Pst race CYR23, accompanied by an increased fungal biomass”
t0“Wheat Gene TaATG8j”
Claim · model-written summary
Silencing TaATG8j decreased necrotic area per infection site.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- virus-induced gene silencing of all TaATG8j copiese0
- Interactor
- Pste0pathogen
- Gene
- TaATG8je0
- Outcome
- necrotic area per infection sitee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The virus-induced gene silencing of all TaATG8j copies rendered Suwon 11 susceptible to the avirulent Pst race CYR23, accompanied by an increased fungal biomass and a decreased necrotic area per infection site”
t0“Wheat Gene TaATG8j”
The same questions in other papers
loss of function · disease · Triticum aestivum (wheat)Positive and negative results
95 papers in the same direction as this paper · 81 in the opposite direction
- positive
- no effect
- negative
173 papers more in this group
See all 177 papers →loss of function · soil: biology · Triticum aestivum (wheat)Results agree
2 papers in the same direction as this paper
- negative
- negative
inoculation · Puccinia striiformis f. sp. tritici · Triticum aestivum (wheat)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.