Abstract
With no lysine (K) (WNK) kinases comprise a family of serine/threonine kinases belonging to an evolutionary branch of the eukaryotic kinome. These special kinases contain a unique active site and are found in a wide range of eukaryotes. The model plant Arabidopsis has been reported to have 11 WNK members, of which WNK8 functions as a negative regulator of abscisic acid (ABA) signaling. Here, we found that the expression of WNK8 is post-transcriptionally regulated through an upstream open reading frame (uORF) found in its 5′ untranslated region (5′-UTR). This uORF has been predicted to encode a conserved peptide named CPuORF58 in both monocotyledons and dicotyledons. The analysis of the published ribosome footprinting studies and the study of the frameshift CPuORF58 peptide with altered repression capability suggested that this uORF causes ribosome stalling. Plants transformed with the native WNK8 promoter driving WNK8 expression were comparable with wild-type plants, whereas the plants transformed with a similar construct with mutated CPuORF58 start codon were less sensitive to ABA. In addition, WNK8 and its downstream target RACK1 were found to synergistically coordinate ABA signaling rather than antagonistically modulating glucose response and flowering in plants. Collectively, these results suggest that the WNK8 expression must be tightly regulated to fulfill the demands of ABA response in plants.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
WNK8 expression is regulated after transcription by an upstream open reading frame.
Not reportedTaxon
Verbatim
e0“the expression of WNK8 is post-transcriptionally regulated through an upstream open reading frame (uORF) found in its 5′ untranslated region (5′-UTR).”
Claim · model-written summary
The WNK8 uORF was predicted to encode conserved CPuORF58 in monocots and dicots.
Verbatim
e0“This uORF has been predicted to encode a conserved peptide named CPuORF58 in both monocotyledons and dicotyledons.”
Claim · model-written summary
Ribosome-footprint and frameshift analyses suggest the uORF stalls ribosomes.
Not reportedTaxon
Verbatim
e0“The analysis of the published ribosome footprinting studies and the study of the frameshift CPuORF58 peptide with altered repression capability suggested that this uORF causes ribosome stalling.”
Claim · model-written summary
Plants with native WNK8 promoter constructs resembled wild-type plants.
Verbatim
e0“Plants transformed with the native WNK8 promoter driving WNK8 expression were comparable with wild-type plants”
Claim · model-written summary
Plants with a mutated CPuORF58 start codon were less sensitive to ABA.
- Taxon
- plantsViridiplantaee0
- Treatment
- mutated CPuORF58 start codone0
- Gene
- CPuORF58e0
- Outcome
- sensitive to ABAe0
- Direction
- lesse0
Not reportedComparator
Verbatim
e0“plants transformed with a similar construct with mutated CPuORF58 start codon were less sensitive to ABA.”
Claim · model-written summary
WNK8 and RACK1 synergistically coordinate ABA signaling.
- Taxon
- plantsViridiplantaee0
- Gene
- WNK8 and its downstream target RACK1e0
- Outcome
- ABA signalinge0
Verbatim
e0“WNK8 and its downstream target RACK1 were found to synergistically coordinate ABA signaling rather than antagonistically modulating glucose response and flowering in plants.”
Claim · model-written summary
WNK8 and RACK1 did not antagonistically modulate glucose response or flowering.
- Taxon
- plantsViridiplantaee0
- Gene
- WNK8 and its downstream target RACK1e0
- Outcome
- glucose response and floweringe0
Verbatim
e0“WNK8 and its downstream target RACK1 were found to synergistically coordinate ABA signaling rather than antagonistically modulating glucose response and flowering in plants.”
Claim · model-written summary
The authors infer that WNK8 expression needs tight regulation for ABA response.
- Taxon
- plantsViridiplantaee0
- Gene
- WNK8e0
- Outcome
- ABA responsee0
Verbatim
e0“the WNK8 expression must be tightly regulated to fulfill the demands of ABA response in plants.”
The same questions in other papers
loss of function · molecular: metabolites · Viridiplantae (plants)Positive and negative results
8 papers in the same direction as this paper · 5 in the opposite direction
- positive
- positive
- positive
10 papers more in this group
See all 14 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.