Abstract
Petals and leaves share common evolutionary origins but have different phenotypic characteristics, such as the absence of stomata in the petals of most angiosperm species. Plant NAC transcription factor, NAP, is involved in ABA responses and regulates senescence-associated genes, and especially those that affect stomatal movement. However, the regulatory mechanisms and significance of NAP action in senescing astomatous petals is unclear. A major limiting factor is failure of flower opening and accelerated senescence. Our goal is to understand the finely regulatory mechanism of dehydration tolerance and aging in rose flowers. We functionally characterized RhNAP, an AtNAP-like transcription factor gene that is induced by dehydration and aging in astomatous rose petals. Cytokinins (CKs) are known to delay petal senescence and we found that a cytokinin oxidase/dehydrogenase gene 6 (RhCKX6) shares similar expression patterns with RhNAP. Silencing of RhNAP or RhCKX6 expression in rose petals by virus induced gene silencing markedly reduced petal dehydration tolerance and delayed petal senescence. Endogenous CK levels in RhNAP- or RhCKX6-silenced petals were significantly higher than those of the control. Moreover, RhCKX6 expression was reduced in RhNAP-silenced petals. This suggests that the expression of RhCKX6 is regulated by RhNAP. Yeast one-hybrid experiments and electrophoresis mobility shift assays showed that RhNAP binds to the RhCKX6 promoter in heterologous in vivo system and in vitro, respectively. Furthermore, the expression of putative signal transduction and downstream genes of ABA-signaling pathways were also reduced due to the repression of PP2C homolog genes by RhNAP in rose petals. Taken together, our study indicates that the RhNAP/RhCKX6 interaction represents a regulatory step enhancing dehydration tolerance in young rose petals and accelerating senescence in mature petals in a stomata-independent manner.
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Claims 7
Claim · model-written summary
Silencing RhNAP or RhCKX6 in rose petals markedly reduced petal dehydration tolerance.
- Taxon
- rosee0
- Treatment
- virus induced gene silencinge1
- Gene
- RhNAP or RhCKX6e1
- Outcome
- petal dehydration tolerancee1
- Direction
- reducede1
Not reportedComparator
Verbatim
e0“induced by dehydration and aging in astomatous rose petals”
e1“Silencing of RhNAP or RhCKX6 expression in rose petals by virus induced gene silencing markedly reduced petal dehydration tolerance and delayed petal senescence”
Claim · model-written summary
Silencing RhNAP or RhCKX6 in rose petals delayed petal senescence.
- Taxon
- rosee0
- Treatment
- virus induced gene silencinge1
- Gene
- RhNAP or RhCKX6e1
- Outcome
- petal senescencee1
- Direction
- delayede1
Not reportedComparator
Verbatim
e0“induced by dehydration and aging in astomatous rose petals”
e1“Silencing of RhNAP or RhCKX6 expression in rose petals by virus induced gene silencing markedly reduced petal dehydration tolerance and delayed petal senescence”
Claim · model-written summary
Endogenous cytokinin levels were higher in RhNAP- or RhCKX6-silenced petals than in controls.
Verbatim
e0“induced by dehydration and aging in astomatous rose petals”
e2“Endogenous CK levels in RhNAP- or RhCKX6-silenced petals were significantly higher than those of the control”
Claim · model-written summary
RhCKX6 expression was reduced in RhNAP-silenced rose petals.
Not reportedComparator
Verbatim
e0“induced by dehydration and aging in astomatous rose petals”
e3“RhCKX6 expression was reduced in RhNAP-silenced petals”
Claim · model-written summary
RhNAP binds the RhCKX6 promoter in vivo and in vitro.
Verbatim
e0“induced by dehydration and aging in astomatous rose petals”
e4“RhNAP binds to the RhCKX6 promoter in heterologous in vivo system and in vitro”
Claim · model-written summary
Expression of ABA-signalling signal transduction and downstream genes was reduced in rose petals through repression of PP2C homolog genes by RhNAP.
Verbatim
e5“the expression of putative signal transduction and downstream genes of ABA-signaling pathways were also reduced due to the repression of PP2C homolog genes by RhNAP in rose petals”
Claim · model-written summary
The RhNAP/RhCKX6 interaction enhances dehydration tolerance in young rose petals and accelerates senescence in mature petals.
Verbatim
e6“the RhNAP/RhCKX6 interaction represents a regulatory step enhancing dehydration tolerance in young rose petals and accelerating senescence in mature petals”
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