Abstract
Heat stress is a major abiotic stress for plants, which can generate a range of biochemical and genetic responses. In 'Ponkan' mandarin fruit, hot air treatment (HAT) accelerates the degradation of citric acid. However, the transcriptional regulatory mechanisms of citrate degradation in response to HAT remain to be elucidated. Here, 17 heat shock transcription factor sequences were isolated, and dual-luciferase assays were employed to investigate whether the encoded proteins that could trans-activate the promoters of key genes in the GABA shunt, involved in citrate metabolism. We identified four heat shock transcription factors (CitHsfA7, CitHsfA3, CitHsfA4b and CitHsfA8) that showed trans-activation effects on CitAco3, CitIDH3 and CitGAD4, respectively. Transient expression of the CitHsfs in citrus fruits indicated that CitHsfA7 was the only factor that resulted in a significant lowering of the citric acid content, and these results were confirmed by a virus-induced gene silencing system (VIGS). Sub-cellar localization showed that CitHsfA7 is located in the nucleus and is capable of binding directly to a putative HSE in the CitAco3 promoter and enhance its expression. We proposed that the induction of CitHsfA7 transcript level contributes to citric acid degradation in citrus fruit, via modulation of CitAco3 in response to HAT.
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Claims 7
Claim · model-written summary
Hot air treatment accelerates citric acid degradation in Ponkan mandarin fruit.
- Taxon
- mandarine0
- Material
- 'Ponkan'e0
- Organ / stage
- fruite0
- Treatment
- hot air treatment (HAT)e0
- Outcome
- degradation of citric acide0
- Direction
- acceleratese0
Not reportedComparator
Verbatim
e0“In 'Ponkan' mandarin fruit, hot air treatment (HAT) accelerates the degradation of citric acid.”
Claim · model-written summary
Seventeen citrus heat shock transcription factor sequences were isolated.
CitrusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“17 heat shock transcription factor sequences were isolated”
t0“Citrus”
Claim · model-written summary
Four CitHsf proteins trans-activated CitAco3, CitIDH3 and CitGAD4 promoters.
- Gene
- CitHsfA7, CitHsfA3, CitHsfA4b and CitHsfA8e0
- Outcome
- trans-activation effects on CitAco3, CitIDH3 and CitGAD4e0
- Value
- foure0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We identified four heat shock transcription factors (CitHsfA7, CitHsfA3, CitHsfA4b and CitHsfA8) that showed trans-activation effects on CitAco3, CitIDH3 and CitGAD4, respectively.”
t0“Citrus”
Claim · model-written summary
Transient CitHsfA7 expression significantly lowered citrus fruit citric acid content.
- Organ / stage
- fruitse0
- Treatment
- Transient expression of the CitHsfse0
- Gene
- CitHsfA7e0
- Outcome
- citric acid contente0
- Direction
- loweringe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient expression of the CitHsfs in citrus fruits indicated that CitHsfA7 was the only factor that resulted in a significant lowering of the citric acid content”
Claim · model-written summary
CitHsfA7 localizes to the nucleus.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sub-cellar localization showed that CitHsfA7 is located in the nucleus”
t0“Citrus”
Claim · model-written summary
CitHsfA7 binds the CitAco3 promoter and enhances its expression.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“is capable of binding directly to a putative HSE in the CitAco3 promoter and enhance its expression.”
t0“Citrus”
Claim · model-written summary
The authors propose that CitHsfA7 induction promotes fruit citric acid degradation through CitAco3 under heat treatment.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We proposed that the induction of CitHsfA7 transcript level contributes to citric acid degradation in citrus fruit, via modulation of CitAco3 in response to HAT.”
The same questions in other papers
transgenic · molecular: metabolites · CitrusPositive and negative results
2 in the opposite direction
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.