Abstract
The traditional Japanese fermented pickle, takuan-zuke, is produced via dehydration and salt-aging of the Japanese winter radish root (Raphanus sativus L.). It has been reported that γ-aminobutyric acid (GABA; antihypertensive factor) accumulates during this production. Herein, the results of the comprehensive study (metabolites, enzyme activity, and gene expression levels were investigated) on the mechanism of branched-chain amino acid (BCAA) synthesis and glucosinolate (GLS)-myrosinase system of daikon dehydration have been reported. BCAAs, GLS, and their precursors were temporarily upregulated, and the genes linked to BCAA, GLS, and isothiocyanate synthesis (BCAT4, MAM3, IPMDH1, RMB1, RMB2, and GRS1) were upregulated during daikon dehydration. BCAAs and GLS accumulated in daikon during dehydration owing to the upregulation of genes, encoding these synthases as a stress response. These results suggest that the biological response of daikon adds characteristic flavor and health functionality to takuan-zuke and helps optimize the processing parameters to produce pickles with improved health benefits. PRACTICAL APPLICATIONS: Takuan-zuke is a popular pickle in Japan, which is produced from a Japanese winter radish root (Raphanus sativus L.), commonly referred to as daikon in Japan. Takuan-zuke is produced by dehydrating daikon either by the process of salt-pressing (shio-oshi) or sun-drying (hoshi). The processes influence the variations in the nutritional value of the final product. The results reveal that both the daikon dehydration processes can be followed to generate increased amounts of health-promoting components (e.g., branched-chain amino acid and glucosinolate) in takuan-zuke.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
BCAAs, GLS and their precursors were temporarily upregulated during daikon dehydration.
- Taxon
- daikon
Raphanus sativus var. longipinnatuse0 - Treatment
- dehydratione0
- Outcome
- BCAAs, GLS, and their precursorse0
- Direction
- upregulatede0
- Qualifier
- temporarilye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BCAAs, GLS, and their precursors were temporarily upregulated, and the genes linked to BCAA, GLS, and isothiocyanate synthesis (BCAT4, MAM3, IPMDH1, RMB1, RMB2, and GRS1) were upregulated during daikon dehydration.”
Claim · model-written summary
BCAT4, MAM3 and IPMDH1 were upregulated during daikon dehydration.
- Taxon
- daikonRaphanus sativus var. longipinnatuse0
- Treatment
- dehydratione0
- Gene
- BCAT4, MAM3, IPMDH1e0
- Outcome
- genes linked to BCAA, GLS, and isothiocyanate synthesise0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the genes linked to BCAA, GLS, and isothiocyanate synthesis (BCAT4, MAM3, IPMDH1, RMB1, RMB2, and GRS1) were upregulated during daikon dehydration”
Claim · model-written summary
RMB1, RMB2 and GRS1 were upregulated during daikon dehydration.
- Taxon
- daikonRaphanus sativus var. longipinnatuse0
- Treatment
- dehydratione0
- Gene
- RMB1, RMB2, and GRS1e0
- Outcome
- genes linked to BCAA, GLS, and isothiocyanate synthesise0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the genes linked to BCAA, GLS, and isothiocyanate synthesis (BCAT4, MAM3, IPMDH1, RMB1, RMB2, and GRS1) were upregulated during daikon dehydration”
Claim · model-written summary
Stress-induced synthase gene upregulation was asserted to cause BCAA and GLS accumulation in dehydrating daikon.
- Taxon
- daikonRaphanus sativus var. longipinnatuse0
- Treatment
- dehydratione0
- Outcome
- BCAAs and GLS accumulatede0
- Qualifier
- upregulation of genes, encoding these synthases as a stress responsee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BCAAs and GLS accumulated in daikon during dehydration owing to the upregulation of genes, encoding these synthases as a stress response.”
Claim · model-written summary
The authors infer that daikon's biological response contributes characteristic flavor and health functionality to takuan-zuke.
- Taxon
- daikonRaphanus sativus var. longipinnatuse0
- Outcome
- characteristic flavor and health functionalitye0
- Qualifier
- biological response of daikone0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results suggest that the biological response of daikon adds characteristic flavor and health functionality to takuan-zuke and helps optimize the processing parameters to produce pickles with improved health benefits.”
Claim · model-written summary
Both daikon dehydration processes are recommended for generating more health-promoting components in takuan-zuke.
- Taxon
- daikonRaphanus sativus var. longipinnatuse0
- Outcome
- both the daikon dehydration processese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The results reveal that both the daikon dehydration processes can be followed to generate increased amounts of health-promoting components (e.g., branched-chain amino acid and glucosinolate) in takuan-zuke.”
The same questions in other papers
drought · quality: phytochemicals · Raphanus sativus (radish)Positive and negative results
1 in the opposite direction
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.