Abstract
Fire blight is a severe bacterial plant disease that affects important chain-of-value fruit trees such as pear and apple trees. This disease is caused by Erwinia amylovora, a quarantine phytopathogenic bacterium, which, although highly distributed worldwide, still lacks efficient control measures. The green revolution paradigm demands sustainable agriculture practices, for which antimicrobial peptides (AMPs) have recently caught much attention. The goal of this work was to disclose the bioactivity of three peptides mixtures (BP100:RW-BP100, BP100:CA-M, and RW-BP100:CA-M), against three strains of E. amylovora representing distinct genotypes and virulence (LMG 2024, Ea 630 and Ea 680). The three AMPs’ mixtures were assayed at eight different equimolar concentrations ranging from 0.25 to 6 μM (1:1). Results showed MIC and MBC values between 2.5 and 4 μM for every AMP mixture and strain. Regarding cell viability, flow cytometry and alamarBlue reduction, showed high reduction (>25%) of viable cells after 30 min of AMP exposure, depending on the peptide mixture and strain assayed. Hypersensitive response in tobacco plants showed that the most efficient AMPs mixtures and concentrations caused low to no reaction of the plant. Altogether, the AMPs mixtures studied are better treatment solutions to control fire blight disease than the same AMPs applied individually.
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Claims 4
Claim · model-written summary
Minimum inhibitory and bactericidal values of the AMP mixtures against Erwinia amylovora were between 2.5 and 4 μM.
- Taxon
- pear and apple trees
Malus domestica_Poirier_Fleurs_et_feuilles.jpg?width=60)
Pyrus communise0 - Treatment
- AMP mixturee1
- Interactor
- Erwinia amylovora
Erwinia amylovorae5pathogen - Outcome
- MIC and MBC valuese1
- Value
- between 2.5 and 4 μMe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Poirier_Fleurs_et_feuilles.jpg?width=500)
Verbatim
e0“Fire blight is a severe bacterial plant disease that affects important chain-of-value fruit trees such as pear and apple trees”
e1“Results showed MIC and MBC values between 2.5 and 4 μM for every AMP mixture and strain”
e5“This disease is caused by Erwinia amylovora, a quarantine phytopathogenic bacterium”
Claim · model-written summary
AMP exposure reduced viable Erwinia amylovora cells by more than 25% after 30 min.
- Taxon
- pear and apple treesMalus domesticaPyrus communise0
- Treatment
- AMP exposuree2
- Interactor
- Erwinia amylovoraErwinia amylovorae5pathogen
- Outcome
- viable cellse2
- Direction
- reductione2
- Value
- >25%e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fire blight is a severe bacterial plant disease that affects important chain-of-value fruit trees such as pear and apple trees”
e2“showed high reduction (>25%) of viable cells after 30 min of AMP exposure”
e5“This disease is caused by Erwinia amylovora, a quarantine phytopathogenic bacterium”
Claim · model-written summary
The most efficient AMP mixtures caused low to no hypersensitive response in tobacco plants.
- Taxon
- tobacco
Nicotiana tabacume3 - Treatment
- most efficient AMPs mixtures and concentrationse3
- Outcome
- Hypersensitive responsee3
- Direction
- low to no reactione3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Hypersensitive response in tobacco plants showed that the most efficient AMPs mixtures and concentrations caused low to no reaction of the plant”
Claim · model-written summary
The AMP mixtures are better treatments than the same AMPs applied individually to control fire blight.
- Taxon
- pear and apple treesMalus domesticaPyrus communise0
- Treatment
- AMPs mixturese4
- Interactor
- Erwinia amylovoraErwinia amylovorae5pathogen
- Outcome
- control fire blight diseasee4
- Direction
- bettere4
- Comparator
- the same AMPs applied individuallye4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fire blight is a severe bacterial plant disease that affects important chain-of-value fruit trees such as pear and apple trees”
e4“the AMPs mixtures studied are better treatment solutions to control fire blight disease than the same AMPs applied individually”
e5“This disease is caused by Erwinia amylovora, a quarantine phytopathogenic bacterium”
The same questions in other papers
antimicrobial agent · Erwinia amylovora · Malus domestica (apple)Results agree
2 papers in the same direction as this paper
- positive
- positive
antimicrobial agent · disease · Nicotiana tabacum (tobacco)Results agree
2 papers in the same direction as this paper
- positive
- positive
antimicrobial agent · Erwinia amylovora · Pyrus communis (pear)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.