Abstract
Cryptochrome 1 and 2 (CRY1 and CRY2) are blue light receptors involved in the regulation of hypocotyl elongation, cotyledon expansion, and flowering time in Arabidopsisthaliana. Two cryptochrome-interacting proteins, Blue-light Inhibitor of Cryptochrome 1 and 2 (BIC1 and BIC2), have been found in Arabidopsis. BIC1 plays critical roles in suppressing the physiological activities of CRY2, which include the blue light-dependent dimerization, phosphorylation, photobody formation, and degradation process, but the functional characterization of BIC protein in other crops has not yet been performed. To investigate the function of BIC protein in rice (Oryza sativa), two homologous genes of Arabidopsis BIC1 and BIC2, namely OsBIC1 and OsBIC2 (OsBICs), were identified. The overexpression of OsBIC1 and OsBIC2 led to increased leaf sheath length, whereas mutations in OsBIC1 displayed shorter leaf sheath in a blue light intensity-dependent manner. OsBIC1 regulated blue light-induced leaf sheath elongation through direct interaction with OsCRY1a, OsCRY1b, and OsCRY2 (OsCRYs). Longitudinal sections of the second leaf sheath demonstrated that OsBIC1 and OsCRYs controlled leaf sheath length by influencing the ratio of epidermal cells with different lengths. RNA-sequencing (RNA-seq) and quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) analysis further proved that OsBIC1 and OsCRYs regulated similar transcriptome changes in regulating Gibberellic Acids (GA)-responsive pathway. Taken together, these results suggested that OsBIC1 and OsCRYs worked together to regulate epidermal cell elongation and control blue light-induced leaf sheath elongation through the GA-responsive pathway.
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Claims 8
Claim · model-written summary
OsBIC1 and OsBIC2 were identified as rice homologues of Arabidopsis BIC genes.
- Taxon
- rice
Oryza sativat0 - Gene
- OsBIC1 and OsBIC2e0
- Outcome
- identifiede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“two homologous genes of Arabidopsis BIC1 and BIC2, namely OsBIC1 and OsBIC2 (OsBICs), were identified”
t0“rice (Oryza sativa)”
Claim · model-written summary
Overexpression of OsBIC1 increased leaf sheath length.
- Taxon
- riceOryza sativat0
- Treatment
- overexpression of OsBIC1e0
- Gene
- OsBIC1e0
- Outcome
- leaf sheath lengthe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The overexpression of OsBIC1 and OsBIC2 led to increased leaf sheath length”
t0“rice (Oryza sativa)”
Claim · model-written summary
Overexpression of OsBIC2 increased leaf sheath length.
- Taxon
- riceOryza sativat0
- Treatment
- overexpression of OsBIC1 and OsBIC2e0
- Gene
- OsBIC2e0
- Outcome
- leaf sheath lengthe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The overexpression of OsBIC1 and OsBIC2 led to increased leaf sheath length”
t0“rice (Oryza sativa)”
Claim · model-written summary
OsBIC1 mutation shortened the leaf sheath in a blue light dependent manner.
- Taxon
- riceOryza sativat0
- Treatment
- mutations in OsBIC1e0
- Gene
- OsBIC1e0
- Outcome
- leaf sheathe0
- Direction
- shortere0
- Qualifier
- blue light intensity-dependent mannere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mutations in OsBIC1 displayed shorter leaf sheath in a blue light intensity-dependent manner”
t0“rice (Oryza sativa)”
Claim · model-written summary
OsBIC1 directly interacts with OsCRY1a, OsCRY1b and OsCRY2 in regulating sheath elongation.
- Taxon
- riceOryza sativat0
- Gene
- OsBIC1e0
- Outcome
- blue light-induced leaf sheath elongatione0
- Qualifier
- direct interaction with OsCRY1a, OsCRY1b, and OsCRY2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsBIC1 regulated blue light-induced leaf sheath elongation through direct interaction with OsCRY1a, OsCRY1b, and OsCRY2 (OsCRYs)”
t0“rice (Oryza sativa)”
Claim · model-written summary
OsBIC1 and OsCRYs affected the distribution of epidermal cell lengths in the second sheath.
- Taxon
- riceOryza sativat0
- Gene
- OsBIC1 and OsCRYse0
- Outcome
- leaf sheath lengthe0
- Qualifier
- ratio of epidermal cells with different lengthse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsBIC1 and OsCRYs controlled leaf sheath length by influencing the ratio of epidermal cells with different lengths”
t0“rice (Oryza sativa)”
Claim · model-written summary
OsBIC1 and OsCRYs produced similar transcriptome changes in the GA responsive pathway.
- Taxon
- riceOryza sativat0
- Gene
- OsBIC1 and OsCRYse0
- Outcome
- similar transcriptome changese0
- Qualifier
- Gibberellic Acids (GA)-responsive pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsBIC1 and OsCRYs regulated similar transcriptome changes in regulating Gibberellic Acids (GA)-responsive pathway”
t0“rice (Oryza sativa)”
Claim · model-written summary
The authors inferred joint regulation of epidermal elongation and light induced sheath elongation through the GA pathway.
- Taxon
- riceOryza sativat0
- Gene
- OsBIC1 and OsCRYse0
- Outcome
- epidermal cell elongation and control blue light-induced leaf sheath elongatione0
- Qualifier
- GA-responsive pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsBIC1 and OsCRYs worked together to regulate epidermal cell elongation and control blue light-induced leaf sheath elongation through the GA-responsive pathway”
t0“rice (Oryza sativa)”