Abstract
Tobacco curly shoot virus (TbCSV) belongs to the genus Begomovirus of the family Geminiviridae , and causes leaf curling and curly shoot symptoms in tobacco and tomato crops. MicroRNAs (miRNAs) are pivotal modulators of plant development and host-virus interactions. However, the relationship between TbCSV infection and miRNAs accumulation has not been well investigated. The present study was conducted to analyze different expressions of miRNAs in Nicotiana benthamiana in response to the infection of TbCSV via small RNAs sequencing. The results showed that 15 up-regulated miRNAs and 12 down-regulated miRNAs were differentially expressed in TbCSV infected N. benthamiana , and nbe-miR167b-3p was down-regulated. To decipher the relationship between nbe-miR167b-3p expression and the accumulations of TbCSV DNA, pCVA mediation of miRNA overexpression and PVX based short tandem target mimic (STTM) were used in this study. It was found that overexpression of nbe-miR167b-3p attenuated leaf curling symptom of TbCSV and decreased viral DNA accumulation, but suppression of nbe-miR167b-3p expression enhanced the symptoms and accumulation of TbCSV. PRCP , the target gene of nbe-miR167b-3p, was silenced in plants using VIGS and this weakened the viral symptoms and DNA accumulation of TbCSV in the plants. Overall, this study clarified the effect of nbe-miR167b-3p on plant defense during TbCSV infection, and provided a framework to reveal the molecular mechanisms of miRNAs between plants and viruses.
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Claims 10
Claim · model-written summary
Small RNA sequencing found 15 up-regulated miRNAs differentially expressed in TbCSV-infected N. benthamiana.
- Taxon
- N. benthamiana
Nicotiana benthamianae1 - Interactor
- TbCSVe1pathogen
- Outcome
- up-regulated miRNAse1
- Value
- 15e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“15 up-regulated miRNAs and 12 down-regulated miRNAs were differentially expressed in TbCSV infected N. benthamiana”
Claim · model-written summary
Small RNA sequencing found 12 down-regulated miRNAs differentially expressed in TbCSV-infected N. benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae1
- Interactor
- TbCSVe1pathogen
- Outcome
- down-regulated miRNAse1
- Value
- 12e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“15 up-regulated miRNAs and 12 down-regulated miRNAs were differentially expressed in TbCSV infected N. benthamiana”
Claim · model-written summary
nbe-miR167b-3p was down-regulated in TbCSV-infected N. benthamiana.
- Taxon
- N. benthamianaNicotiana benthamianae1
- Interactor
- TbCSVe1pathogen
- Gene
- nbe-miR167b-3pe1
- Outcome
- down-regulatede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“12 down-regulated miRNAs were differentially expressed in TbCSV infected N. benthamiana , and nbe-miR167b-3p was down-regulated”
Claim · model-written summary
Overexpression of nbe-miR167b-3p attenuated the leaf curling symptom of TbCSV.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- overexpression of nbe-miR167b-3pe1
- Interactor
- TbCSVe1pathogen
- Gene
- nbe-miR167b-3pe1
- Outcome
- leaf curling symptom of TbCSVe1
- Direction
- attenuatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e1“overexpression of nbe-miR167b-3p attenuated leaf curling symptom of TbCSV and decreased viral DNA accumulation”
Claim · model-written summary
Overexpression of nbe-miR167b-3p decreased TbCSV viral DNA accumulation.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- overexpression of nbe-miR167b-3pe1
- Interactor
- TbCSVe1pathogen
- Gene
- nbe-miR167b-3pe1
- Outcome
- viral DNA accumulatione1
- Direction
- decreasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e1“overexpression of nbe-miR167b-3p attenuated leaf curling symptom of TbCSV and decreased viral DNA accumulation”
Claim · model-written summary
Suppression of nbe-miR167b-3p expression enhanced TbCSV symptoms.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- suppression of nbe-miR167b-3p expressione1
- Interactor
- TbCSVe1pathogen
- Gene
- nbe-miR167b-3pe1
- Outcome
- symptomse1
- Direction
- enhancede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e1“suppression of nbe-miR167b-3p expression enhanced the symptoms and accumulation of TbCSV”
Claim · model-written summary
Suppression of nbe-miR167b-3p expression enhanced TbCSV accumulation.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- suppression of nbe-miR167b-3p expressione1
- Interactor
- TbCSVe1pathogen
- Gene
- nbe-miR167b-3pe1
- Outcome
- accumulation of TbCSVe1
- Direction
- enhancede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e1“suppression of nbe-miR167b-3p expression enhanced the symptoms and accumulation of TbCSV”
Claim · model-written summary
Silencing PRCP, the target gene of nbe-miR167b-3p, by VIGS weakened the viral symptoms of TbCSV.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- silenced in plants using VIGSe1
- Interactor
- TbCSVe1pathogen
- Gene
- PRCPe1
- Outcome
- viral symptomse1
- Direction
- weakenede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e1“PRCP , the target gene of nbe-miR167b-3p, was silenced in plants using VIGS and this weakened the viral symptoms and DNA accumulation of TbCSV in the plants”
Claim · model-written summary
Silencing PRCP by VIGS weakened TbCSV DNA accumulation.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Treatment
- silenced in plants using VIGSe1
- Interactor
- TbCSVe1pathogen
- Gene
- PRCPe1
- Outcome
- DNA accumulation of TbCSVe1
- Direction
- weakenede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Nicotiana benthamiana”
e1“PRCP , the target gene of nbe-miR167b-3p, was silenced in plants using VIGS and this weakened the viral symptoms and DNA accumulation of TbCSV in the plants”
Claim · model-written summary
TbCSV down-regulates nbe-miR167b-3p expression to facilitate its infection in N. benthamiana.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat0
- Interactor
- Tobacco curly shoot virusTobacco curly shoot virust0pathogen
- Gene
- nbe-miR167b-3pt0
- Outcome
- Infectiont0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Tobacco curly shoot virus Down-Regulated the Expression of nbe-miR167b-3p to Facilitate Its Infection in Nicotiana benthamiana”
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See all 143 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.