Abstract
ICE (inducer of CBF expression) is a positive regulator of cold signaling pathway in plants. Identification of ICE transcription factors is important for the sustainable development of the natural rubber planting industry in nontraditional regions where sudden cold waves often occur. In this study, five ICE genes were isolated from genome of rubber tree (Hevea brasiliensis Muell. Arg.) for analysing tolerance to cold stress. They shared an ICE-specific region in the highly conserved bHLH-ZIP domain and were localized in the nucleus. The HbICEs were different in transcript abundance and expression patterns in response to cold and drought stresses and among different rubber tree clones. Generally, the expression level of HbICEs was significantly higher in the cold-tolerant rubber tree clones than that in the cold-sensitive rubber tree clones. Overexpression of HbICE1, HbICE2, and HbICE4 significantly enhanced the cold tolerance of transgenic Arabidopsis and tobacco, which showed a significant increase in chlorophyll content and decrease in relative water content and conductivity at the early stage of cold stress in comparison with wild-type plants. Furthermore, overexpression of HbICE2 and HbICE4, but also HbICE1 enhanced drought tolerance in transgenic Arabidopsis. The cold tolerance of rubber tree clones is positively controlled by the expression level of HbICE1, HbICE2, and HbICE4.
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Claims 15
Claim · model-written summary
Five ICE genes were isolated from rubber tree.
- Taxon
- rubber tree
Hevea brasiliensise0 - Outcome
- five ICE genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“five ICE genes were isolated from genome of rubber tree (Hevea brasiliensis Muell. Arg.)”
Claim · model-written summary
The ICE genes shared a conserved domain region.
- Taxon
- rubber treeHevea brasiliensist0
- Gene
- bHLH-ZIP domaine0
- Outcome
- ICE-specific regione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“They shared an ICE-specific region in the highly conserved bHLH-ZIP domain”
t0“Rubber Tree”
Claim · model-written summary
The ICE factors localized to the nucleus.
- Taxon
- rubber treeHevea brasiliensist0
- Outcome
- localized in the nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“were localized in the nucleus”
t0“Rubber Tree”
Claim · model-written summary
HbICE transcript abundance and expression patterns differed across cold and drought stress and clones.
- Taxon
- rubber treeHevea brasiliensise0
- Material
- rubber tree clonese0
- Treatment
- cold and drought stressese0
- Gene
- HbICEse0
- Outcome
- transcript abundance and expression patternse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The HbICEs were different in transcript abundance and expression patterns in response to cold and drought stresses and among different rubber tree clones”
Claim · model-written summary
HbICE expression was higher in cold-tolerant than cold-sensitive rubber tree clones.
- Taxon
- rubber treeHevea brasiliensise0
- Material
- cold-tolerant rubber tree clonese0
- Gene
- HbICEse0
- Outcome
- expression levele0
- Direction
- highere0
- Comparator
- cold-sensitive rubber tree clonese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression level of HbICEs was significantly higher in the cold-tolerant rubber tree clones than that in the cold-sensitive rubber tree clones”
Claim · model-written summary
HbICE1 overexpression enhanced cold tolerance in transgenic Arabidopsis and tobacco.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- Overexpression of HbICE1e0
- Gene
- HbICE1e0
- Outcome
- cold tolerancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of HbICE1, HbICE2, and HbICE4 significantly enhanced the cold tolerance of transgenic Arabidopsis and tobacco”
Claim · model-written summary
HbICE2 overexpression enhanced cold tolerance in transgenic Arabidopsis and tobacco.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- Overexpression of HbICE1, HbICE2, and HbICE4e0
- Gene
- HbICE2e0
- Outcome
- cold tolerancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of HbICE1, HbICE2, and HbICE4 significantly enhanced the cold tolerance of transgenic Arabidopsis and tobacco”
Claim · model-written summary
HbICE4 overexpression enhanced cold tolerance in transgenic Arabidopsis and tobacco.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Overexpression of HbICE1, HbICE2, and HbICE4e0
- Gene
- HbICE4e0
- Outcome
- cold tolerancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of HbICE1, HbICE2, and HbICE4 significantly enhanced the cold tolerance of transgenic Arabidopsis and tobacco”
Claim · model-written summary
The transgenic plants had increased chlorophyll content under early cold stress.
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- chlorophyll contente0
- Direction
- increasee0
- Comparator
- wild-type plantse0
Not reportedOrgan / stageTreatment
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of HbICE1, HbICE2, and HbICE4 significantly enhanced the cold tolerance of transgenic Arabidopsis and tobacco, which showed a significant increase in chlorophyll content and decrease in relative water content and conductivity at the early stage of cold stress in comparison with wild-type plants”
Claim · model-written summary
The transgenic plants had decreased relative water content under early cold stress.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- relative water contente0
- Direction
- decreasee0
- Comparator
- wild-type plantse0
Not reportedTreatment
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of HbICE1, HbICE2, and HbICE4 significantly enhanced the cold tolerance of transgenic Arabidopsis and tobacco, which showed a significant increase in chlorophyll content and decrease in relative water content and conductivity at the early stage of cold stress in comparison with wild-type plants”
Claim · model-written summary
The transgenic plants had decreased conductivity under early cold stress.
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- conductivitye0
- Direction
- decreasee0
- Comparator
- wild-type plantse0
Not reportedTreatment
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of HbICE1, HbICE2, and HbICE4 significantly enhanced the cold tolerance of transgenic Arabidopsis and tobacco, which showed a significant increase in chlorophyll content and decrease in relative water content and conductivity at the early stage of cold stress in comparison with wild-type plants”
Claim · model-written summary
HbICE2 overexpression enhanced drought tolerance in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- overexpression of HbICE2e0
- Gene
- HbICE2e0
- Outcome
- drought tolerancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of HbICE2 and HbICE4, but also HbICE1 enhanced drought tolerance in transgenic Arabidopsis”
Claim · model-written summary
HbICE4 overexpression enhanced drought tolerance in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- overexpression of HbICE2 and HbICE4e0
- Gene
- HbICE4e0
- Outcome
- drought tolerancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of HbICE2 and HbICE4, but also HbICE1 enhanced drought tolerance in transgenic Arabidopsis”
Claim · model-written summary
HbICE1 overexpression enhanced drought tolerance in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- overexpression of HbICE2 and HbICE4, but also HbICE1e0
- Gene
- HbICE1e0
- Outcome
- drought tolerancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of HbICE2 and HbICE4, but also HbICE1 enhanced drought tolerance in transgenic Arabidopsis”
Claim · model-written summary
Rubber tree cold tolerance is positively controlled by HbICE1, HbICE2 and HbICE4 expression.
- Taxon
- rubber treeHevea brasiliensise0
- Gene
- HbICE1e0
- Outcome
- cold tolerancee0
- Direction
- positively controllede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cold tolerance of rubber tree clones is positively controlled by the expression level of HbICE1, HbICE2, and HbICE4”