Abstract
Abstract Low temperature can affect the growth and development of plants through changes in DNA demethylation patterns. Another known effect of low temperature is the accumulation of anthocyanin pigments. However, it is not known whether the two phenomena are linked, specifically whether DNA demethylation participates in anthocyanin accumulation in response to low-temperature stress. The ROS1 gene is involved in plant DNA demethylation and influences methylation levels in response to low-temperature stress. In this study, using RNA sequencing, we detected the transcription levels of MdROS1, as well as those of anthocyanin biosynthesis-related genes, correlate with the anthocyanin content in apple (Malus domestica), at low temperature. Genomic bisulfite sequencing showed that the methylation levels of the promoters of the anthocyanin-related genes MdCHS, MdCHI, MdF3′H, MdANS, MdUFGT, and MdMYB10 decreased in apple leaves after low-temperature treatment. Similar expression and methylation results were found in apple fruit. Transiently silencing MdROS1 in the leaves and fruit of apple cultivars inhibited the accumulation of anthocyanins and led to decreased expression of anthocyanin biosynthetic genes, and the opposite results were detected in MdROS1-overexpressing leaves and fruit. A promoter binding assay showed that the conserved RRD-DME domains of MdROS1 bind directly to the promoters of MdF3′H and MdUFGT. Taken together, these results suggest that ROS1 affects the anthocyanin biosynthetic pathway by decreasing the methylation level of anthocyanin-related gene promoters, thereby increasing their expression and increasing anthocyanin accumulation.
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Claims 6
Claim · model-written summary
MdROS1 and anthocyanin gene transcription correlated with anthocyanin content at low temperature.
- Taxon
- apple
Malus domesticae0 - Gene
- MdROS1e0
- Outcome
- anthocyanin contente0
- Qualifier
- at low temperaturee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transcription levels of MdROS1, as well as those of anthocyanin biosynthesis-related genes, correlate with the anthocyanin content in apple (Malus domestica), at low temperature”
Claim · model-written summary
Low temperature reduced methylation of anthocyanin gene promoters in apple leaves.
- Taxon
- appleMalus domesticae0
- Organ / stage
- leavese0
- Treatment
- low-temperature treatmente0
- Gene
- MdCHS, MdCHI, MdF3′H, MdANS, MdUFGT, and MdMYB10e0
- Outcome
- methylation levelse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the methylation levels of the promoters of the anthocyanin-related genes MdCHS, MdCHI, MdF3′H, MdANS, MdUFGT, and MdMYB10 decreased in apple leaves after low-temperature treatment”
Claim · model-written summary
Silencing MdROS1 inhibited anthocyanin accumulation in apple leaves and fruit.
- Taxon
- appleMalus domesticae0
- Organ / stage
- leaves and fruite0
- Treatment
- Transiently silencing MdROS1e0
- Gene
- MdROS1e0
- Outcome
- accumulation of anthocyaninse0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transiently silencing MdROS1 in the leaves and fruit of apple cultivars inhibited the accumulation of anthocyanins”
Claim · model-written summary
Silencing MdROS1 decreased anthocyanin biosynthetic gene expression.
- Taxon
- appleMalus domesticae0
- Treatment
- Transiently silencing MdROS1e0
- Gene
- MdROS1e0
- Outcome
- expression of anthocyanin biosynthetic genese0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transiently silencing MdROS1 in the leaves and fruit of apple cultivars inhibited the accumulation of anthocyanins and led to decreased expression of anthocyanin biosynthetic genes”
Claim · model-written summary
MdROS1 overexpression produced opposite anthocyanin and expression results.
- Taxon
- appleMalus domesticat0
- Organ / stage
- leaves and fruite0
- Treatment
- MdROS1-overexpressinge0
- Gene
- MdROS1e0
- Outcome
- opposite resultse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the opposite results were detected in MdROS1-overexpressing leaves and fruit”
t0“apple”
Claim · model-written summary
MdROS1 domains bound MdF3′H and MdUFGT promoters directly.
- Taxon
- appleMalus domesticat0
- Gene
- MdROS1e0
- Outcome
- bind directly to the promoters of MdF3′H and MdUFGTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the conserved RRD-DME domains of MdROS1 bind directly to the promoters of MdF3′H and MdUFGT”
t0“apple”
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