Abstract
Crown roots (CRs) are major components of the rice root system. They form at the basal node of the shoot, and their development is greatly influenced by environmental factors. Ammonium nitrogen is known to impact plant root development through ammonium transporters (AMTs), but it remains unclear whether ammonium and AMTs play roles in rice CR formation. In this study, we revealed a significant role of ammonium, rather than nitrate, in regulating rice CR development. High ammonium supply increases CR formation but inhibits CR elongation. Genetic evidence showed that ammonium regulation of CR development relies on ammonium uptake mediated jointly by ammonium transporters OsAMT1;1, OsAMT1;2; OsAMT1;3, and OsAMT2;1, but not on root acidification which was the result of ammonium uptake. OsAMTs are also needed for glutamine-induced CR formation. Furthermore, we showed that polar auxin transport dependent on the PIN auxin efflux carriers acts downstream of ammonium uptake and assimilation to activate local auxin signaling at CR primordia, in turn promoting CR formation. Taken together, our results highlight a critical role for OsAMTs in cooperatively regulating CR formation through regulating auxin transport under nitrogen-rich conditions.
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Claims 7
Claim · model-written summary
High ammonium supply increases rice crown root formation.
- Taxon
- rice
Oryza sativat0 - Treatment
- High ammonium supplye0
- Outcome
- CR formatione0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High ammonium supply increases CR formation”
t0“rice crown root formation”
Claim · model-written summary
High ammonium supply inhibits rice crown root elongation.
- Taxon
- riceOryza sativat0
- Treatment
- High ammonium supplye0
- Outcome
- CR elongatione0
- Direction
- inhibitse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High ammonium supply increases CR formation but inhibits CR elongation”
t0“rice crown root formation”
Claim · model-written summary
Ammonium, rather than nitrate, regulates rice crown root development.
- Taxon
- riceOryza sativae0
- Treatment
- ammoniume0
- Outcome
- CR developmente0
- Comparator
- nitratee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a significant role of ammonium, rather than nitrate, in regulating rice CR development”
Claim · model-written summary
Ammonium regulation of rice crown roots relies jointly on four ammonium transporters.
- Taxon
- riceOryza sativat0
- Gene
- OsAMT1;1, OsAMT1;2; OsAMT1;3, and OsAMT2;1e0
- Outcome
- CR developmente0
- Qualifier
- ammonium uptakee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ammonium regulation of CR development relies on ammonium uptake mediated jointly by ammonium transporters OsAMT1;1, OsAMT1;2; OsAMT1;3, and OsAMT2;1”
t0“rice crown root formation”
Claim · model-written summary
Root acidification does not mediate ammonium regulation of rice crown root development.
- Taxon
- riceOryza sativat0
- Outcome
- root acidificatione0
- Qualifier
- ammonium uptakee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“not on root acidification which was the result of ammonium uptake”
t0“rice crown root formation”
Claim · model-written summary
Ammonium transporters are needed for glutamine-induced rice crown root formation.
- Taxon
- riceOryza sativat0
- Treatment
- glutaminee0
- Gene
- OsAMTse0
- Outcome
- CR formatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsAMTs are also needed for glutamine-induced CR formation”
t0“rice crown root formation”
Claim · model-written summary
PIN-dependent polar auxin transport activates local auxin signaling at rice crown root primordia and promotes crown root formation.
- Taxon
- riceOryza sativat0
- Organ / stage
- CR primordiae0
- Gene
- PIN auxin efflux carrierse0
- Outcome
- CR formatione0
- Qualifier
- local auxin signalinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“polar auxin transport dependent on the PIN auxin efflux carriers acts downstream of ammonium uptake and assimilation to activate local auxin signaling at CR primordia, in turn promoting CR formation”
t0“rice crown root formation”
The same questions in other papers
nitrogen · growth · Oryza sativa (rice)Positive and negative results
401 papers in the same direction as this paper · 101 in the opposite direction · 8 reporting no difference from an alternative
- negative
- negative
- negative
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