Paper · 2022 · Journal of Agricultural Science

Combination of Azospirillum and Bradyrhizobium on Inoculant Formulation Improve Nitrogen Biological Fixation in Soybean

Mateus Manara Picoli; João Vitor Gomes Pasquetto; Cesar Yoshio Muraoka; et al.

5
claims
10
verbatim quotes
8
citations
3
research areas
✓
Open access · cc-by

Abstract

Co-inoculation in soybean with Azospirillum brasilense and Bradyrhizobium spp. is a consolidated practice to ensure nitrogen supply and maximize productivity and sustainability. This study aimed to evaluate the agronomic efficiency of the co-inoculation in soybean, via seed or in-furrow, of doses of the mixed inoculant containing Bradyrhizobium spp. and A. brasilense in the same formulation. The experiments were conducted under field conditions in four different edaphoclimatic regions to compare seven treatments: Control without nitrogen (N) and inoculant (T1), 200 kg ha-1 of mineral N fertilizer (T2), 100 mL of standard liquid inoculant of Bradyrhizobium spp. (T3) in 50 kg-1 seeds, 100 mL (T4) or 200 mL (T5) of the mixed liquid inoculant in 50 kg-1 seeds, 200 mL (T6) or 300 mL (T7) ha-1 of the mixed inoculant applied in-furrow. Nodulation characteristics (number and mass of nodules) and carbon and N metabolism (dry mass, N content, and N accumulation in the aerial part) were evaluated at the phenological stage R2 (full flowering—there is an open flower at one of the two uppermost nodes). At maturity (R8—full maturity—95% of the pods have reached their full mature color), yield, the mass of 1,000 grains, N content in the grains, and grain N export were measured. The data were submitted to analysis of variance using the F test and comparison of means was performed using the Duncan test (5%). Under field conditions, the mixed inoculant at doses of 200 mL 50 kg-1 seed or 300 mL ha-1 applied in-furrow were equivalent or superior to the standard inoculant for the total number of nodules, N content in the grains, grain N export, productivity, and mass of 1,000 grains. The use of the mixed inoculant containing Bradyrhizobium spp. and A. brasilense in the same formulation meets the demand of grain N export and resulted in increases of 28%, 18% and 7.4% in soybean productivity compared with control without nitrogen, mineral N fertilizer and standard liquid inoculant of Bradyrhizobium spp. respectively.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 5

Intervention effectobservedoriginal resultmixedmeasured

Claim · model-written summary

Under field conditions the mixed inoculant at 200 mL/50 kg seed or 300 mL/ha in-furrow was equivalent or superior to the standard inoculant for nodule number, grain N content and grain N export.

Taxon
SoybeanGlycine maxt0
Treatment
mixed inoculant at doses of 200 mL 50 kg-1 seed or 300 mL ha-1 applied in-furrowe1
Outcome
total number of nodules, N content in the grains, grain N exporte1
Direction
equivalent or superiore1
Comparator
standard inoculante1
Setting
Under field conditionse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e1

“Under field conditions, the mixed inoculant at doses of 200 mL 50 kg-1 seed or 300 mL ha-1 applied in-furrow were equivalent or superior to the standard inoculant for the total number of nodules, N content in the grains, grain N export, productivity, and mass of 1,000 grains”

✓ located verbatim in the source · abstract, characters 1414–1689 · show in text ↑
t0

“Nitrogen Biological Fixation in Soybean”

✓ located verbatim in the source · title, characters 80–119 · show in text ↑
Intervention effectobservedoriginal resultmixedmeasured

Claim · model-written summary

Under field conditions the mixed inoculant at 200 mL/50 kg seed or 300 mL/ha in-furrow was equivalent or superior to the standard inoculant for productivity and 1,000-grain mass.

Taxon
SoybeanGlycine maxt0
Treatment
mixed inoculant at doses of 200 mL 50 kg-1 seed or 300 mL ha-1 applied in-furrowe1
Outcome
productivity, and mass of 1,000 grainse1
Direction
equivalent or superiore1
Comparator
standard inoculante1
Setting
Under field conditionse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e1

“Under field conditions, the mixed inoculant at doses of 200 mL 50 kg-1 seed or 300 mL ha-1 applied in-furrow were equivalent or superior to the standard inoculant for the total number of nodules, N content in the grains, grain N export, productivity, and mass of 1,000 grains”

✓ located verbatim in the source · abstract, characters 1414–1689 · show in text ↑
t0

“Nitrogen Biological Fixation in Soybean”

✓ located verbatim in the source · title, characters 80–119 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The mixed Bradyrhizobium and A. brasilense inoculant increased soybean productivity by 28% compared with the control without nitrogen.

Taxon
SoybeanGlycine maxt0
Treatment
mixed inoculant containing Bradyrhizobium spp. and A. brasilensee2
Interactor
Bradyrhizobium spp.Bradyrhizobiume2symbiont
Second interactor
A. brasilenseAzospirillum brasilensee2symbiont
Outcome
productivitye2
Direction
increasese2
Value
28%e2
Comparator
control without nitrogene2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗
Bradyrhizobium
Bradyrhizobium · Interactor · picture of the genus (one of its species)Louisa Howard - Dartmouth Electron Microscope Facility · Public domain · Wikimedia Commons ↗
Azospirillum brasilense
Azospirillum brasilense · Second interactorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

e2

“The use of the mixed inoculant containing Bradyrhizobium spp. and A. brasilense in the same formulation meets the demand of grain N export and resulted in increases of 28%, 18% and 7.4% in soybean productivity compared with control without nitrogen, mineral N fertilizer and standard liquid inoculant of Bradyrhizobium spp. respectively”

✓ located verbatim in the source · abstract, characters 1691–2027 · show in text ↑
t0

“Nitrogen Biological Fixation in Soybean”

✓ located verbatim in the source · title, characters 80–119 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The mixed Bradyrhizobium and A. brasilense inoculant increased soybean productivity by 18% compared with mineral N fertilizer.

Taxon
SoybeanGlycine maxt0
Treatment
mixed inoculant containing Bradyrhizobium spp. and A. brasilensee2
Interactor
Bradyrhizobium spp.Bradyrhizobiume2symbiont
Second interactor
A. brasilenseAzospirillum brasilensee2symbiont
Outcome
productivitye2
Direction
increasese2
Value
18%e2
Comparator
mineral N fertilizere2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗
Bradyrhizobium
Bradyrhizobium · Interactor · picture of the genus (one of its species)Louisa Howard - Dartmouth Electron Microscope Facility · Public domain · Wikimedia Commons ↗
Azospirillum brasilense
Azospirillum brasilense · Second interactorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

e2

“The use of the mixed inoculant containing Bradyrhizobium spp. and A. brasilense in the same formulation meets the demand of grain N export and resulted in increases of 28%, 18% and 7.4% in soybean productivity compared with control without nitrogen, mineral N fertilizer and standard liquid inoculant of Bradyrhizobium spp. respectively”

✓ located verbatim in the source · abstract, characters 1691–2027 · show in text ↑
t0

“Nitrogen Biological Fixation in Soybean”

✓ located verbatim in the source · title, characters 80–119 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The mixed Bradyrhizobium and A. brasilense inoculant increased soybean productivity by 7.4% compared with the standard liquid Bradyrhizobium inoculant.

Taxon
SoybeanGlycine maxt0
Treatment
mixed inoculant containing Bradyrhizobium spp. and A. brasilensee2
Interactor
Bradyrhizobium spp.Bradyrhizobiume2symbiont
Second interactor
A. brasilenseAzospirillum brasilensee2symbiont
Outcome
productivitye2
Direction
increasese2
Value
7.4%e2
Comparator
standard liquid inoculant of Bradyrhizobium spp.e2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗
Bradyrhizobium
Bradyrhizobium · Interactor · picture of the genus (one of its species)Louisa Howard - Dartmouth Electron Microscope Facility · Public domain · Wikimedia Commons ↗
Azospirillum brasilense
Azospirillum brasilense · Second interactorCristiane Colodel · CC BY 4.0 · iNaturalist ↗

Verbatim

e2

“The use of the mixed inoculant containing Bradyrhizobium spp. and A. brasilense in the same formulation meets the demand of grain N export and resulted in increases of 28%, 18% and 7.4% in soybean productivity compared with control without nitrogen, mineral N fertilizer and standard liquid inoculant of Bradyrhizobium spp. respectively”

✓ located verbatim in the source · abstract, characters 1691–2027 · show in text ↑
t0

“Nitrogen Biological Fixation in Soybean”

✓ located verbatim in the source · title, characters 80–119 · show in text ↑

Azospirillum brasilense · yield · Glycine max (soybean)Results differ

18 papers: 14 positive, 4 negative or no effect

13 papers in the same direction as this paper · 3 in the opposite direction · 1 reporting no difference from an alternative

  1. no effect
  2. positive
  3. positive

14 papers more in this group

See all 18 papers →

Bradyrhizobium · yield · Glycine max (soybean)Results differ

36 papers: 32 positive, 4 negative or no effect

31 papers in the same direction as this paper · 3 in the opposite direction · 1 reporting no difference from an alternative

  1. no effect
  2. negative
  3. no effect

32 papers more in this group

See all 36 papers →

inoculation · yield · Glycine max (soybean)Positive and negative results

566 papers: 491 positive, 75 negative or no effect

490 papers in the same direction as this paper · 63 in the opposite direction · 12 reporting no difference from an alternative

  1. no effect
  2. positive
  3. positive

562 papers more in this group

See all 566 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.