Paper · 1974 · American Journal of Botany

Ozone Sensitivity of Leaves: Relationship to Leaf Water Content, Gas Transfer Resistance, and Anatomical Characteristics

Lance S. Evans; Irwin P. Ting

6
claims
11
verbatim quotes
41
citations
2
research areas
—
Not open access

Abstract

Relative water content, resistance to gas transfer, stomatal spacing, and other characteristics of primary bean leaves were studied in relation to ozone sensitivity and injury. Cells of primary bean leaves are maximally sensitive to ozone exposure 9–10 days after germination under our experimental conditions. The stage of maximum sensitivity was not correlated with changes in stomatal number or resistance on either adaxial or abaxial leaf surfaces. It was deduced that bean leaf sensitivity was a function of more internal circumstances, and gas exchange was never the limiting factor through the developmental period studied. Changes in resistance were not significantly altered by ozone levels that produced no visible injury. After exposure to high ozone doses, a decrease in adaxial resistance occurred apparently as a result of palisade and epidermal cell lysis. Normally most gas exchange occurs through the adaxial surface. A 10 % decrease in relative water content accompanying a 60-min ozone exposure of 0.55 ppm could not be explained physiologically on the basis of cell injury as no visible leaf injury occurred.

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Claims 6

Observationobservedoriginal resultmeasured

Claim · model-written summary

Cells of primary bean leaves are maximally sensitive to ozone 9–10 days after germination.

Taxon
beane1
Organ / stage
primary bean leavese2
Outcome
maximally sensitive to ozone exposuree2
Qualifier
9–10 days after germinatione2

Verbatim

e1

“characteristics of primary bean leaves were studied in relation to ozone sensitivity and injury”

✓ located verbatim in the source · abstract, characters 80–175 · show in text ↑
e2

“Cells of primary bean leaves are maximally sensitive to ozone exposure 9–10 days after germination”

✓ located verbatim in the source · abstract, characters 177–275 · show in text ↑
Associationobservedoriginal resultno effectmeasured

Claim · model-written summary

The stage of maximum ozone sensitivity was not correlated with changes in stomatal number or resistance on either leaf surface.

Taxon
beane1
Outcome
stage of maximum sensitivitye3
Comparator
changes in stomatal number or resistancee3

Not reportedDirection

Verbatim

e1

“characteristics of primary bean leaves were studied in relation to ozone sensitivity and injury”

✓ located verbatim in the source · abstract, characters 80–175 · show in text ↑
e3

“The stage of maximum sensitivity was not correlated with changes in stomatal number or resistance on either adaxial or abaxial leaf surfaces”

✓ located verbatim in the source · abstract, characters 311–451 · show in text ↑
Mechanismauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

Bean leaf sensitivity to ozone is a function of more internal circumstances, and gas exchange was never the limiting factor.

Taxon
beane4
Outcome
bean leaf sensitivitye4
Qualifier
more internal circumstancese4

Verbatim

e4

“It was deduced that bean leaf sensitivity was a function of more internal circumstances, and gas exchange was never the limiting factor”

✓ located verbatim in the source · abstract, characters 453–588 · show in text ↑
Associationobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

Changes in leaf resistance were not significantly altered by ozone levels that produced no visible injury.

Taxon
beane1
Treatment
ozone levels that produced no visible injurye5
Outcome
Changes in resistancee5
Direction
no_changeinferrede5

Verbatim

e1

“characteristics of primary bean leaves were studied in relation to ozone sensitivity and injury”

✓ located verbatim in the source · abstract, characters 80–175 · show in text ↑
e5

“Changes in resistance were not significantly altered by ozone levels that produced no visible injury”

✓ located verbatim in the source · abstract, characters 631–731 · show in text ↑
Intervention effectauthor interpretationoriginal resultmeasured

Claim · model-written summary

After high ozone doses, adaxial resistance decreased, apparently as a result of palisade and epidermal cell lysis.

Taxon
beane1
Treatment
high ozone dosese6
Outcome
adaxial resistancee6
Direction
decreasee6
Qualifier
palisade and epidermal cell lysise6

Not reportedComparator

Verbatim

e1

“characteristics of primary bean leaves were studied in relation to ozone sensitivity and injury”

✓ located verbatim in the source · abstract, characters 80–175 · show in text ↑
e6

“After exposure to high ozone doses, a decrease in adaxial resistance occurred apparently as a result of palisade and epidermal cell lysis”

✓ located verbatim in the source · abstract, characters 733–870 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

A 10 % decrease in relative water content accompanied a 60-min ozone exposure of 0.55 ppm.

Taxon
beane1
Treatment
60-min ozone exposure of 0.55 ppme7
Outcome
relative water contente7
Direction
decreasee7
Value
10 %e7

Not reportedComparator

Verbatim

e1

“characteristics of primary bean leaves were studied in relation to ozone sensitivity and injury”

✓ located verbatim in the source · abstract, characters 80–175 · show in text ↑
e7

“A 10 % decrease in relative water content accompanying a 60-min ozone exposure of 0.55 ppm”

✓ located verbatim in the source · abstract, characters 935–1025 · show in text ↑