Paper · 1911 · Botanical Gazette

Studies on the Relation of the Living Cells to the Transpiration and Sap-Flow in Cyperus. II

James Bertram Overton

20
claims
43
verbatim quotes
1
citations
2
research areas
—
Not open access

Abstract

1. Stems of Cyperus cut and placed in water wither sooner than when a certain portion, not to exceed 20 cm., has been killed by steam. 2. When 20 cm. of the stem are killed by steam, the leaves wither in about 8 days, that is, in about the same time as the control plants. 3. The longer the portion of the stem killed with steam, the sooner the leaves above wither and dry. When 25 to 30 cm. of the stem are killed with steam, the leaves wither in 3-5 days. 4. No matter how long the section killed may be, the leaves on steamed stems never wither quite so quickly as those cut and not placed in water, but under the same conditions of light, temperature, and air moisture. 5. In Cyperus sufficient water to maintain the leaves turgid for 3-18 days will rise through a stem 15-60 cm. high, with a section 5-30 cm. long which has been killed with steam. 6. A certain amount of water is raised through the steamed portion, but it gradually diminishes in quantity from day to day, until the leaves become air dry (about 11 per cent of their dry weight of moisture). 7. The diminished water supply is partly due to a partial blocking of the vessels with a gumlike or resinous substance, which probably owes its origin to the disorganization of the contents of the sieve tubes caused by heating the stems. 8. The withering of the leaves above a steamed portion of the stem is probably caused more by the action of deleterious substances introduced into them from the dead cells than from lack of water. These poisonous substances are probably disorganization products caused by heating with steam. 9. The leaves of rooted plants, grown in nutrient solutions containing sterilized decoctions of the same plant, droop in 3-5 days, discolor and dry in 7-8 days. 10. The withering leaves above a portion of the stem killed with steam show all the symptoms of dying, namely, rapid loss of water after treatment, then a more uniform loss, rounding up and discoloration of the chloroplasts, and contraction of the mesophyll protoplasts. The leaves are apparently drying, not so much from lack of water as on account of the death of the cells from other causes. 11. Judging from the behavior and disorganization of the leaves on a stem, a section of which has been killed with steam, it is evident that this method of killing the cells is not a satisfactory one in order to settle the question as to the relation of the living cells to sapflow. 12. Killing a portion of the stem by applying wax heated to 110⚬ C. causes less apparent disorganization of the cells, less injury to the leaves above, and does not cause a marked immediate decrease in the transpiration. 13. Experiments in which 5-10 cm. of the stem are killed by treatment with picric acid, 95 per cent alcohol, or CuSO4, for 36-48 hours show that sufficient quantities of water may ascend through the poisoned portions to supply the transpiration need for a comparatively long period (90 days), and to allow the development of new branches. 14. Certain poisons (picric acid, chromic acid, and HgCl2) may greatly accelerate the amount of water evaporated in poisoned plants. Not all poisons act alike in this respect; HgCl2 causes the greatest amount of increase in water loss.

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The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 20

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Cut Cyperus stems in water wither sooner when a portion up to 20 cm is killed by steam.

Taxon
CyperusCyperuse1
Organ / stage
Stemse1
Treatment
killed by steame1
Outcome
withere1
Direction
soonere1
Comparator
Stems of Cyperus cut and placed in watere1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cyperus
Cyperus · Taxon · picture of the genus (one of its species)Miwasatoshi · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“Stems of Cyperus cut and placed in water wither sooner than when a certain portion, not to exceed 20 cm., has been killed by steam”

✓ located verbatim in the source · abstract, characters 3–133 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

With 20 cm of stem killed by steam, leaves wither in about 8 days, about the same time as control plants.

Taxon
CyperusCyperuse0
Treatment
20 cm. of the stem are killed by steame1
Outcome
withere1
Direction
about the same timee1
Value
8 dayse1
Comparator
control plantse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cyperus
Cyperus · Taxon · picture of the genus (one of its species)Miwasatoshi · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Stems of Cyperus”

✓ located verbatim in the source · abstract, characters 3–19 · show in text ↑
e1

“When 20 cm. of the stem are killed by steam, the leaves wither in about 8 days, that is, in about the same time as the control plants”

✓ located verbatim in the source · abstract, characters 138–271 · show in text ↑
Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

The longer the steam-killed stem portion, the sooner the leaves above wither and dry.

Taxon
CyperusCyperuse0
Treatment
portion of the stem killed with steame1
Outcome
leaves above wither and drye1
Direction
soonere1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cyperus
Cyperus · Taxon · picture of the genus (one of its species)Miwasatoshi · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Stems of Cyperus”

✓ located verbatim in the source · abstract, characters 3–19 · show in text ↑
e1

“The longer the portion of the stem killed with steam, the sooner the leaves above wither and dry”

✓ located verbatim in the source · abstract, characters 276–372 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

With 25 to 30 cm of stem killed by steam, leaves wither in 3-5 days.

Taxon
CyperusCyperuse0
Treatment
25 to 30 cm. of the stem are killed with steame1
Outcome
withere1
Value
3-5 dayse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cyperus
Cyperus · Taxon · picture of the genus (one of its species)Miwasatoshi · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Stems of Cyperus”

✓ located verbatim in the source · abstract, characters 3–19 · show in text ↑
e1

“When 25 to 30 cm. of the stem are killed with steam, the leaves wither in 3-5 days”

✓ located verbatim in the source · abstract, characters 374–456 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Leaves on steam-killed stems never wither as quickly as leaves on stems cut and not placed in water, under the same conditions.

Taxon
CyperusCyperuse0
Treatment
steamed stemse1
Outcome
withere1
Direction
never wither quite so quicklye1
Comparator
those cut and not placed in watere1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cyperus
Cyperus · Taxon · picture of the genus (one of its species)Miwasatoshi · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Stems of Cyperus”

✓ located verbatim in the source · abstract, characters 3–19 · show in text ↑
e1

“No matter how long the section killed may be, the leaves on steamed stems never wither quite so quickly as those cut and not placed in water, but under the same conditions of light, temperature, and air moisture”

✓ located verbatim in the source · abstract, characters 461–672 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Sufficient water to keep leaves turgid for 3-18 days rises through a 15-60 cm stem containing a 5-30 cm steam-killed section.

Taxon
CyperusCyperuse1
Treatment
a section 5-30 cm. long which has been killed with steame1
Outcome
sufficient water to maintain the leaves turgide1
Value
15-60 cme1
Qualifier
for 3-18 dayse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cyperus
Cyperus · Taxon · picture of the genus (one of its species)Miwasatoshi · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“In Cyperus sufficient water to maintain the leaves turgid for 3-18 days will rise through a stem 15-60 cm. high, with a section 5-30 cm. long which has been killed with steam”

✓ located verbatim in the source · abstract, characters 677–851 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Water raised through the steamed stem portion gradually diminishes day by day until the leaves become air dry (about 11 per cent moisture).

Taxon
CyperusCyperuse0
Treatment
steamed portione1
Outcome
water is raised through the steamed portione1
Direction
gradually diminishese1
Value
11 per cente1
Qualifier
until the leaves become air drye1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cyperus
Cyperus · Taxon · picture of the genus (one of its species)Miwasatoshi · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Stems of Cyperus”

✓ located verbatim in the source · abstract, characters 3–19 · show in text ↑
e1

“A certain amount of water is raised through the steamed portion, but it gradually diminishes in quantity from day to day, until the leaves become air dry (about 11 per cent of their dry weight of moisture)”

✓ located verbatim in the source · abstract, characters 856–1061 · show in text ↑
Mechanismauthor interpretationoriginal resultnegativemeasured

Claim · model-written summary

The diminished water supply is partly due to partial blocking of the vessels by a gummy or resinous substance from heat-disorganized sieve tube contents.

Taxon
CyperusCyperuse0
Outcome
diminished water supplye1
Qualifier
partial blocking of the vessels with a gumlike or resinous substancee1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cyperus
Cyperus · Taxon · picture of the genus (one of its species)Miwasatoshi · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Stems of Cyperus”

✓ located verbatim in the source · abstract, characters 3–19 · show in text ↑
e1

“The diminished water supply is partly due to a partial blocking of the vessels with a gumlike or resinous substance, which probably owes its origin to the disorganization of the contents of the sieve tubes caused by heating the stems”

✓ located verbatim in the source · abstract, characters 1066–1299 · show in text ↑
Mechanismauthor interpretationoriginal resultnegativemeasured

Claim · model-written summary

Withering of leaves above a steamed stem portion is probably caused more by deleterious substances from dead cells than by lack of water.

Taxon
CyperusCyperuse0
Outcome
withering of the leaves above a steamed portion of the steme1
Qualifier
deleterious substances introduced into them from the dead cellse1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cyperus
Cyperus · Taxon · picture of the genus (one of its species)Miwasatoshi · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Stems of Cyperus”

✓ located verbatim in the source · abstract, characters 3–19 · show in text ↑
e1

“The withering of the leaves above a steamed portion of the stem is probably caused more by the action of deleterious substances introduced into them from the dead cells than from lack of water”

✓ located verbatim in the source · abstract, characters 1304–1496 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Leaves of plants grown in nutrient solution with sterilized decoctions of the same plant droop within 3-5 days.

Taxon
CyperusCyperuse0
Treatment
sterilized decoctions of the same plante1
Outcome
leavese1
Direction
droope1
Value
3-5 dayse1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cyperus
Cyperus · Taxon · picture of the genus (one of its species)Miwasatoshi · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Stems of Cyperus”

✓ located verbatim in the source · abstract, characters 3–19 · show in text ↑
e1

“The leaves of rooted plants, grown in nutrient solutions containing sterilized decoctions of the same plant, droop in 3-5 days, discolor and dry in 7-8 days”

✓ located verbatim in the source · abstract, characters 1596–1752 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Leaves of plants grown in nutrient solution with sterilized decoctions of the same plant discolor and dry within 7-8 days.

Taxon
CyperusCyperuse0
Treatment
sterilized decoctions of the same plante1
Outcome
leavese1
Direction
discolor and drye1
Value
7-8 dayse1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cyperus
Cyperus · Taxon · picture of the genus (one of its species)Miwasatoshi · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Stems of Cyperus”

✓ located verbatim in the source · abstract, characters 3–19 · show in text ↑
e1

“The leaves of rooted plants, grown in nutrient solutions containing sterilized decoctions of the same plant, droop in 3-5 days, discolor and dry in 7-8 days”

✓ located verbatim in the source · abstract, characters 1596–1752 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Withering leaves above a steam-killed stem portion show all the symptoms of dying.

Taxon
CyperusCyperuse0
Treatment
a portion of the stem killed with steame1
Outcome
symptoms of dyinge1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cyperus
Cyperus · Taxon · picture of the genus (one of its species)Miwasatoshi · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Stems of Cyperus”

✓ located verbatim in the source · abstract, characters 3–19 · show in text ↑
e1

“The withering leaves above a portion of the stem killed with steam show all the symptoms of dying”

✓ located verbatim in the source · abstract, characters 1758–1855 · show in text ↑
Recommendationauthor interpretationoriginal resultnegativebasis n/a

Claim · model-written summary

The authors judge steam killing of stem cells not a satisfactory method for settling the relation of living cells to sap flow.

Taxon
CyperusCyperuse0
Outcome
this method of killing the cells is not a satisfactory onee1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cyperus
Cyperus · Taxon · picture of the genus (one of its species)Miwasatoshi · CC BY-SA 4.0 · Wikimedia Commons ↗

Schema note from the model: method suitability judgement by the authors, not a management recommendation

Verbatim

e0

“Stems of Cyperus”

✓ located verbatim in the source · abstract, characters 3–19 · show in text ↑
e1

“it is evident that this method of killing the cells is not a satisfactory one in order to settle the question as to the relation of the living cells to sapflow”

✓ located verbatim in the source · abstract, characters 2271–2430 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Killing a stem portion with heated wax causes less apparent disorganization of cells than steam killing.

Taxon
CyperusCyperuse0
Treatment
applying wax heated toe2
Outcome
disorganization of the cellse1
Direction
less apparente1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cyperus
Cyperus · Taxon · picture of the genus (one of its species)Miwasatoshi · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Stems of Cyperus”

✓ located verbatim in the source · abstract, characters 3–19 · show in text ↑
e1

“causes less apparent disorganization of the cells”

✓ located verbatim in the source · abstract, characters 2500–2549 · show in text ↑
e2

“Killing a portion of the stem by applying wax heated to”

✓ located verbatim in the source · abstract, characters 2436–2491 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Killing a stem portion with heated wax causes less injury to the leaves above.

Taxon
CyperusCyperuse0
Treatment
applying wax heated toe2
Outcome
injury to the leaves abovee3
Direction
lesse3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cyperus
Cyperus · Taxon · picture of the genus (one of its species)Miwasatoshi · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Stems of Cyperus”

✓ located verbatim in the source · abstract, characters 3–19 · show in text ↑
e2

“Killing a portion of the stem by applying wax heated to”

✓ located verbatim in the source · abstract, characters 2436–2491 · show in text ↑
e3

“less injury to the leaves above”

✓ located verbatim in the source · abstract, characters 2551–2582 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Killing a stem portion with heated wax does not cause a marked immediate decrease in transpiration.

Taxon
CyperusCyperuse0
Treatment
applying wax heated toe2
Outcome
transpiratione4
Direction
decreasee4
Qualifier
does not cause a marked immediatee4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cyperus
Cyperus · Taxon · picture of the genus (one of its species)Miwasatoshi · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Stems of Cyperus”

✓ located verbatim in the source · abstract, characters 3–19 · show in text ↑
e2

“Killing a portion of the stem by applying wax heated to”

✓ located verbatim in the source · abstract, characters 2436–2491 · show in text ↑
e4

“does not cause a marked immediate decrease in the transpiration”

✓ located verbatim in the source · abstract, characters 2588–2651 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Stem sections of 5-10 cm killed with picric acid, 95 per cent alcohol, or CuSO4 for 36-48 hours still let enough water ascend to meet transpiration for about 90 days.

Taxon
CyperusCyperuse0
Treatment
picric acid, 95 per cent alcohol, or CuSO4e6
Outcome
sufficient quantities of water may ascend through the poisoned portionse5
Qualifier
90 dayse5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cyperus
Cyperus · Taxon · picture of the genus (one of its species)Miwasatoshi · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Stems of Cyperus”

✓ located verbatim in the source · abstract, characters 3–19 · show in text ↑
e5

“sufficient quantities of water may ascend through the poisoned portions to supply the transpiration need for a comparatively long period (90 days)”

✓ located verbatim in the source · abstract, characters 2798–2944 · show in text ↑
e6

“5-10 cm. of the stem are killed by treatment with picric acid, 95 per cent alcohol, or CuSO4”

✓ located verbatim in the source · abstract, characters 2678–2770 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Stem sections killed with picric acid, 95 per cent alcohol, or CuSO4 allow development of new branches.

Taxon
CyperusCyperuse0
Treatment
picric acid, 95 per cent alcohol, or CuSO4e6
Outcome
development of new branchese7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cyperus
Cyperus · Taxon · picture of the genus (one of its species)Miwasatoshi · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Stems of Cyperus”

✓ located verbatim in the source · abstract, characters 3–19 · show in text ↑
e6

“5-10 cm. of the stem are killed by treatment with picric acid, 95 per cent alcohol, or CuSO4”

✓ located verbatim in the source · abstract, characters 2678–2770 · show in text ↑
e7

“to allow the development of new branches”

✓ located verbatim in the source · abstract, characters 2950–2990 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Certain poisons (picric acid, chromic acid, HgCl2) may greatly accelerate water evaporation from poisoned plants.

Taxon
CyperusCyperuse0
Treatment
picric acid, chromic acid, and HgCl2e1
Outcome
amount of water evaporatede1
Direction
acceleratee1
Qualifier
may greatlye1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cyperus
Cyperus · Taxon · picture of the genus (one of its species)Miwasatoshi · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Stems of Cyperus”

✓ located verbatim in the source · abstract, characters 3–19 · show in text ↑
e1

“Certain poisons (picric acid, chromic acid, and HgCl2) may greatly accelerate the amount of water evaporated in poisoned plants”

✓ located verbatim in the source · abstract, characters 2996–3123 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

HgCl2 causes the greatest increase in water loss among the tested poisons.

Taxon
CyperusCyperuse0
Treatment
HgCl2e1
Outcome
water losse1
Direction
greatest amount of increasee1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cyperus
Cyperus · Taxon · picture of the genus (one of its species)Miwasatoshi · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Stems of Cyperus”

✓ located verbatim in the source · abstract, characters 3–19 · show in text ↑
e1

“HgCl2 causes the greatest amount of increase in water loss”

✓ located verbatim in the source · abstract, characters 3168–3226 · show in text ↑