Abstract
In this study, a total of 66 UDP-glucose pyrophosphorylase (UGP) (EC 2.7.7.9) genes were identified from the genomes of four cotton species, which are the members of Pfam glycosyltransferase family (PF01702) and catalyze the reaction between glucose-1-phosphate and UTP to produce UDPG. The analysis of evolutionary relationship, gene structure, and expression provides the basis for studies on function of UGP genes in cotton. The evolutionary tree and gene structure analysis revealed that the UGP gene family is evolutionarily conserved. Collinearity and Ka/Ks analysis indicated that amplification of UGP genes is due to repetitive crosstalk generating between new family genes, while being under strong selection pressure. The analysis of cis -acting elements exhibited that UGP genes play important role in cotton growth, development, abiotic and hormonal stresses. Six UGP genes that were highly expressed in cotton fiber at 15 DPA were screened by transcriptome data and qRT-PCR analysis. The addition of low concentrations of IAA and GA3 to ovule cultures revealed that energy efficiency promoted the development of ovules and fiber clusters, and qRT-PCR showed that expression of these six UGP genes was differentially increased. These results suggest that the UGP gene may play an important role in fiber development, and provides the opportunity to plant researchers to explore the mechanisms involve in fiber development in cotton.
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Claims 7
Claim · model-written summary
A total of 66 UGP genes were identified in four cotton genomes.
- Taxon
- cotton
Gossypium hirsutume0 - Gene
- UDP-glucose pyrophosphorylase (UGP)e0
- Outcome
- genes were identifiede0
- Value
- 66e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a total of 66 UDP-glucose pyrophosphorylase (UGP) (EC 2.7.7.9) genes were identified from the genomes of four cotton species”
Claim · model-written summary
The UGP family was evolutionarily conserved.
- Taxon
- Gossypium hirsutumGossypium hirsutumt0
- Gene
- UGP gene familye0
- Outcome
- evolutionarily conservede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the UGP gene family is evolutionarily conserved”
t0“Gossypium hirsutum”
Claim · model-written summary
UGP gene amplification was associated with repetitive crosstalk under strong selection pressure.
- Taxon
- Gossypium hirsutumGossypium hirsutumt0
- Gene
- UGP genese0
- Outcome
- amplification of UGP genese0
- Qualifier
- strong selection pressuree0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“amplification of UGP genes is due to repetitive crosstalk generating between new family genes, while being under strong selection pressure”
t0“Gossypium hirsutum”
Claim · model-written summary
Cis-acting elements suggested a role for UGP genes in cotton growth, development and abiotic and hormonal stresses.
- Taxon
- cottonGossypium hirsutume0
- Gene
- UGP genese0
- Outcome
- growth, development, abiotic and hormonal stressese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“UGP genes play important role in cotton growth, development, abiotic and hormonal stresses”
Claim · model-written summary
Six UGP genes were highly expressed in cotton fiber at 15 DPA.
- Taxon
- cottonGossypium hirsutume0
- Organ / stage
- cotton fibere0
- Gene
- UGP genese0
- Outcome
- highly expressed in cotton fibere0
- Value
- Sixe0
- Qualifier
- 15 DPAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Six UGP genes that were highly expressed in cotton fiber at 15 DPA”
Claim · model-written summary
Low concentrations of IAA and GA3 promoted ovule and fiber cluster development.
- Taxon
- Gossypium hirsutumGossypium hirsutumt0
- Treatment
- low concentrations of IAA and GA3e0
- Outcome
- development of ovules and fiber clusterse0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The addition of low concentrations of IAA and GA3 to ovule cultures revealed that energy efficiency promoted the development of ovules and fiber clusters”
t0“Gossypium hirsutum”
Claim · model-written summary
Expression of the six UGP genes increased differentially after IAA and GA3 addition.
- Taxon
- Gossypium hirsutumGossypium hirsutumt0
- Treatment
- low concentrations of IAA and GA3e1
- Gene
- UGP genese0
- Outcome
- expression of these six UGP genese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“qRT-PCR showed that expression of these six UGP genes was differentially increased”
e1“The addition of low concentrations of IAA and GA3 to ovule cultures”
t0“Gossypium hirsutum”
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