Abstract
Retention of RNA in the nucleus precisely regulates the time and rate of translation and controls transcriptional bursts that can generate profound variability in mRNA levels among identical cells in tissues. In this study, we investigated the function of Cajal bodies (CBs) in RNA retention in A. thaliana leaf nuclei during hypoxia stress was investigated. It was observed that in ncb-1 mutants with a complete absence of CBs, the accumulation of poly(A+) RNA in the leaf nuclei was lower than that in wt under stress. Moreover, unlike in root cells, CBs store less RNA, and RNA retention in the nuclei is much less intense. Our results reveal that the function of CBs in the accumulation of RNA in nuclei under stress depends on the plant organ. Additionally, in ncb-1, retention of introns of mRNA RPB1 (largest subunit of RNA polymerase II) mRNA was observed. However, this isoform is highly accumulated in the nucleus. It thus follows that intron retention in transcripts is more important than CBs for the accumulation of RNA in nuclei. Accumulated mRNAs with introns in the nucleus could escape transcript degradation by NMD (nonsense-mediated mRNA decay). From non-fully spliced mRNAs in ncb-1 nuclei, whose levels increase during hypoxia, introns are removed during reoxygenation. Then, the mRNA is transferred to the cytoplasm, and the RPB1 protein is translated. Despite the accumulation of isoforms in nuclei with retention of introns in reoxygenation, ncb-1 coped much worse with long hypoxia, and manifested faster yellowing and shrinkage of leaves.
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Claims 8
Claim · model-written summary
In ncb-1 mutants lacking Cajal bodies, the accumulation of poly(A+) RNA in leaf nuclei under stress was lower than in wild type.
Verbatim
t0“A. thaliana Leaves”
e0“in ncb-1 mutants with a complete absence of CBs, the accumulation of poly(A+) RNA in the leaf nuclei was lower than that in wt under stress”
Claim · model-written summary
Unlike in root cells, Cajal bodies in leaves store less RNA and RNA retention in the nuclei is much less intense.
Verbatim
t0“A. thaliana Leaves”
e0“unlike in root cells, CBs store less RNA, and RNA retention in the nuclei is much less intense”
Claim · model-written summary
In ncb-1, retention of introns of RPB1 mRNA was observed.
Verbatim
t0“A. thaliana Leaves”
e0“in ncb-1, retention of introns of mRNA RPB1 (largest subunit of RNA polymerase II) mRNA was observed”
Claim · model-written summary
The authors conclude that intron retention in transcripts is more important than Cajal bodies for the accumulation of RNA in nuclei.
Verbatim
t0“A. thaliana Leaves”
e0“intron retention in transcripts is more important than CBs for the accumulation of RNA in nuclei”
Claim · model-written summary
Accumulated mRNAs with introns in the nucleus could escape transcript degradation by NMD.
Verbatim
t0“A. thaliana Leaves”
e0“Accumulated mRNAs with introns in the nucleus could escape transcript degradation by NMD”
Claim · model-written summary
Levels of non-fully spliced mRNAs in ncb-1 nuclei increase during hypoxia.
Verbatim
t0“A. thaliana Leaves”
e0“non-fully spliced mRNAs in ncb-1 nuclei, whose levels increase during hypoxia”
Claim · model-written summary
Introns of the non-fully spliced mRNAs are removed during reoxygenation.
Verbatim
t0“A. thaliana Leaves”
e0“introns are removed during reoxygenation”
Claim · model-written summary
ncb-1 coped much worse with long hypoxia and showed faster yellowing and shrinkage of leaves.
- Taxon
- A. thalianat0
- Material
- ncb-1e0
- Treatment
- long hypoxiae0
- Outcome
- yellowing and shrinkage of leavese0
- Direction
- fastere0
Not reportedComparator
Verbatim
t0“A. thaliana Leaves”
e0“ncb-1 coped much worse with long hypoxia, and manifested faster yellowing and shrinkage of leaves”
The same questions in other papers
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2 papers in the same direction as this paper
- negative
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.