Abstract
Grain filling is a crucial process that affects yield in rice (Oryza sativa L.). Auxin biosynthesis and signaling are closely related to rice yield; therefore, it is important to understand the effects of auxin biosynthesis on rice grain filling to improve crop yield. In this study, we used physiological and molecular strategies to identify the roles of auxin in rice grain filling. Exogenous application of auxin (IAA) or auxin analogues (2, 4-D) to young spikelets and flag leaves improved the seed-setting rate and yield per spike. Furthermore, real-time quantitative PCR assays confirmed that nine members of the OsYUCCA family of auxin biosynthetic genes were upregulated during grain filling, implication that auxin biosynthesis plays a major role in grain development. The specific expression of either Arabidopsis AtYUCCA1 or OsYUCCA2 in the endosperm or leaves resulted in increased expression of OsIAA genes and auxin content of seeds, as well as increased grain filling and seed-setting rate. This result establishes that the auxin content in grains and leaves is important for grain development. Our findings further highlight the potential applications for improving rice yield by elevating targeted gene expression in specific tissues.
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Claims 4
Claim · model-written summary
Exogenous auxin or auxin analogue application to young spikelets and flag leaves improved seed-setting rate and yield per spike in rice.
- Taxon
- rice
Oryza sativae0 - Organ / stage
- young spikelets and flag leavese1
- Treatment
- Exogenous application of auxin (IAA) or auxin analogues (2, 4-D)e1
- Outcome
- seed-setting rate and yield per spikee1
- Direction
- improvede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Grain filling is a crucial process that affects yield in rice (Oryza sativa L.)”
e1“Exogenous application of auxin (IAA) or auxin analogues (2, 4-D) to young spikelets and flag leaves improved the seed-setting rate and yield per spike”
Claim · model-written summary
Nine OsYUCCA auxin biosynthesis genes were upregulated during rice grain filling.
- Taxon
- riceOryza sativae0
- Organ / stage
- grain fillinge2
- Gene
- OsYUCCAe2
- Outcome
- nine members of the OsYUCCA family of auxin biosynthetic genese2
- Direction
- upregulatede2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Grain filling is a crucial process that affects yield in rice (Oryza sativa L.)”
e2“nine members of the OsYUCCA family of auxin biosynthetic genes were upregulated during grain filling”
Claim · model-written summary
Endosperm-specific expression of AtYUCCA1 or OsYUCCA2 increased OsIAA gene expression and seed auxin content in rice.
- Taxon
- riceOryza sativae0
- Organ / stage
- endosperme3
- Treatment
- specific expression of either Arabidopsis AtYUCCA1 or OsYUCCA2 in the endosperm or leavese3
- Gene
- AtYUCCA1 or OsYUCCA2e3
- Outcome
- expression of OsIAA genes and auxin content of seedse3
- Direction
- increasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Grain filling is a crucial process that affects yield in rice (Oryza sativa L.)”
e3“The specific expression of either Arabidopsis AtYUCCA1 or OsYUCCA2 in the endosperm or leaves resulted in increased expression of OsIAA genes and auxin content of seeds, as well as increased grain filling and seed-setting rate”
Claim · model-written summary
Endosperm-specific expression of AtYUCCA1 or OsYUCCA2 increased grain filling and seed-setting rate in rice.
- Taxon
- riceOryza sativae0
- Organ / stage
- endosperme3
- Treatment
- specific expression of either Arabidopsis AtYUCCA1 or OsYUCCA2 in the endosperm or leavese3
- Gene
- AtYUCCA1 or OsYUCCA2e3
- Outcome
- grain filling and seed-setting ratee3
- Direction
- increasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Grain filling is a crucial process that affects yield in rice (Oryza sativa L.)”
e3“The specific expression of either Arabidopsis AtYUCCA1 or OsYUCCA2 in the endosperm or leaves resulted in increased expression of OsIAA genes and auxin content of seeds, as well as increased grain filling and seed-setting rate”
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