Abstract
Auxin plays an important role in regulating plant development, and Auxin/indole acetic acid (Aux/IAA) is a type of auxin-responsive gene and plays an important role in auxin signaling; to date, although 29 Aux/IAA proteins have been reported in Abrabidopsis thaliana, only parts of the Aux/IAA family gene functions have been identified. We previously reported that a bud sport of ‘Longfeng’ (LF) apple (Malus domestica), named ‘Grand longfeng’ (GLF), which showed a larger fruit size than LF, has lower expression of MdAux/IAA2. In this study, we identified the function of the MdAux/IAA2 gene in apple fruit size difference using Agrobacterium-mediated genetic transformation. Overexpression of MdAux/IAA2 decreased the apple flesh callus increment and caused a smaller globular cell size. In addition, overexpression of MdAux/IAA2 in GLF fruit resulted in the reduction of apple fruit size, weight, and cell size, while silencing MdAux/IAA2 in LF apple fruit resulted in an increase in apple fruit weight and cell size. We suggest that the high auxin content depressed the expression of MdAux/IAA2, and that the downregulated expression of MdAux/IAA2 led to the formation of GLF. Our study suggests a mechanism for fruit size regulation in plants and we will explore the transcription factors functioning in this process in the future.
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Claims 4
Claim · model-written summary
Overexpression of MdAux/IAA2 decreased apple flesh callus increment and produced smaller globular cells.
- Taxon
- apple
Malus domesticae0 - Treatment
- Overexpression of MdAux/IAA2e0
- Gene
- MdAux/IAA2e0
- Outcome
- apple flesh callus incremente0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of MdAux/IAA2 decreased the apple flesh callus increment and caused a smaller globular cell size”
t0“The MdAux/IAA2 Transcription Repressor Regulates Cell and Fruit Size in Apple Fruit”
Claim · model-written summary
Overexpression of MdAux/IAA2 in GLF fruit reduced fruit size, weight and cell size.
- Taxon
- appleMalus domesticae1
- Material
- GLFe1
- Treatment
- overexpression of MdAux/IAA2e1
- Gene
- MdAux/IAA2e1
- Outcome
- fruit size, weight, and cell sizee1
- Direction
- reductione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“overexpression of MdAux/IAA2 in GLF fruit resulted in the reduction of apple fruit size, weight, and cell size”
t0“The MdAux/IAA2 Transcription Repressor Regulates Cell and Fruit Size in Apple Fruit”
Claim · model-written summary
Silencing MdAux/IAA2 in LF apple fruit increased fruit weight and cell size.
- Taxon
- appleMalus domesticae2
- Material
- LFe2
- Treatment
- silencing MdAux/IAA2e2
- Gene
- MdAux/IAA2e2
- Outcome
- apple fruit weight and cell sizee2
- Direction
- increasee2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“silencing MdAux/IAA2 in LF apple fruit resulted in an increase in apple fruit weight and cell size”
t0“The MdAux/IAA2 Transcription Repressor Regulates Cell and Fruit Size in Apple Fruit”
Claim · model-written summary
High auxin content may depress MdAux/IAA2 expression, and its downregulation may lead to the GLF fruit size phenotype.
- Taxon
- appleMalus domesticat0
- Gene
- MdAux/IAA2e3
- Compound
- auxine3
- Outcome
- expression of MdAux/IAA2e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“We suggest that the high auxin content depressed the expression of MdAux/IAA2”
t0“The MdAux/IAA2 Transcription Repressor Regulates Cell and Fruit Size in Apple Fruit”
The same questions in other papers
loss of function · cytology · Malus domestica (apple)Positive and negative results
3 in the opposite direction
- no effect
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.