Abstract
Abstract As a versatile compound, myo -inositol plays vital roles in plant biochemistry and physiology. We previously showed that exogenous application of myo -inositol had a positive role in salinity tolerance in Malus hupehensis Rehd. In this study, we used MdMIPS (the rate-limiting gene of myo -inositol biosynthesis) transgenic apple lines to gain new insights into the physiological role of myo -inositol in apple. Decreasing myo -inositol biosynthesis in apple lines by RNA silencing of MdMIPS1/2 led to extensive programmed cell death, which manifested as necrosis of both the leaves and roots and, ultimately, plant death. Necrosis was directly caused by the excessive accumulation of reactive oxygen species, which may be closely associated with the cell wall polysaccharide-mediated increase in salicylic acid and a compromised antioxidant system, and this process was enhanced by an increase in ethylene production. In addition, a high accumulation of sorbitol promoted necrosis. This synergetic interplay between salicylic acid and ethylene was further supported by the fact that increased myo -inositol accumulation significantly delayed leaf senescence in MdMIPS1 -overexpressing apple lines. Taken together, our results indicated that apple myo -inositol regulates reactive oxygen species-induced programmed cell death through salicylic acid-dependent and ethylene-dependent pathways.
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Claims 7
Claim · model-written summary
Silencing MdMIPS1/2 reduced myo-inositol biosynthesis and led to programmed cell death.
- Taxon
- apple
Malus domesticat0 - Treatment
- RNA silencing of MdMIPS1/2e0
- Gene
- MdMIPS1/2e0
- Outcome
- programmed cell deathe0
- Direction
- extensivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Decreasing myo -inositol biosynthesis in apple lines by RNA silencing of MdMIPS1/2 led to extensive programmed cell death”
t0“apple”
Claim · model-written summary
MdMIPS1/2 silencing produced necrosis in leaves and roots and ultimately plant death.
- Taxon
- appleMalus domesticat0
- Treatment
- RNA silencing of MdMIPS1/2e1
- Gene
- MdMIPS1/2e1
- Outcome
- necrosis of both the leaves and roots and, ultimately, plant deathe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which manifested as necrosis of both the leaves and roots and, ultimately, plant death”
e1“RNA silencing of MdMIPS1/2”
t0“apple”
Claim · model-written summary
Excess reactive oxygen species directly caused necrosis.
- Taxon
- appleMalus domesticat0
- Compound
- reactive oxygen speciese0
- Outcome
- Necrosise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Necrosis was directly caused by the excessive accumulation of reactive oxygen species”
t0“apple”
Claim · model-written summary
A cell wall polysaccharide-mediated salicylic acid increase and compromised antioxidant system may be associated with necrosis.
- Taxon
- appleMalus domesticat0
- Compound
- salicylic acide0
- Outcome
- Necrosise1
- Qualifier
- compromised antioxidant systeme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which may be closely associated with the cell wall polysaccharide-mediated increase in salicylic acid and a compromised antioxidant system”
e1“Necrosis was directly caused by the excessive accumulation of reactive oxygen species”
t0“apple”
Claim · model-written summary
Increased ethylene production enhanced the necrosis process.
- Taxon
- appleMalus domesticat0
- Compound
- ethylenee0
- Outcome
- Necrosise1
- Direction
- enhancede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this process was enhanced by an increase in ethylene production”
e1“Necrosis was directly caused by the excessive accumulation of reactive oxygen species”
t0“apple”
Claim · model-written summary
High sorbitol accumulation promoted necrosis.
- Taxon
- appleMalus domesticat0
- Compound
- sorbitole0
- Outcome
- necrosise0
- Direction
- promotede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a high accumulation of sorbitol promoted necrosis”
t0“apple”
Claim · model-written summary
MdMIPS1 overexpression increased myo-inositol accumulation and significantly delayed leaf senescence.
- Taxon
- appleMalus domesticae0
- Treatment
- MdMIPS1 -overexpressinge0
- Gene
- MdMIPS1e0
- Compound
- myo -inositole0
- Outcome
- leaf senescencee0
- Direction
- delayede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased myo -inositol accumulation significantly delayed leaf senescence in MdMIPS1 -overexpressing apple lines”
The same questions in other papers
loss of function · cytology · Malus domestica (apple)Positive and negative results
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- positive
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loss of function · disease · Malus domestica (apple)Positive and negative results
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- positive
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See all 21 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.