Abstract
The WRKY gene family is most widely known as being the key plant transcription factor family involved in various stress responses and affecting plant growth and development. In this study, a total of 86 members of the CsWRKY genes were identified from the tea plant genome. Most of these genes contain several important Cis-regulatory elements in the promoter regions associated with multiple stress-responses. These genes were further classified into three groups, I, II, and III, each with 21, 58, and 7 members, respectively. We showed evidence that tandem duplications, but not the whole genome duplication, are likely to drive the amplification of CsWRKY genes in tea plants. All the 86 CsWRKY genes showed differential expression patterns either in different tissues, or under exposure to diverse abiotic stresses such as drought, cold acclimation, and MeJA treatments. Additionally, the functional roles of two genes, CsWRKY29 and CsWRKY37, were examined under cold stress; and the silencing of these genes resulted in tea plant phenotypes susceptible to cold stress. Moreover, transgenic Arabidopsis lines overexpressing CsWRKY29 and CsWRKY37 genes showed higher survival rates and lower malondialdehyde levels under freezing treatment than the wild type plants. The core findings from this work provide valuable evolutionary pattern of WRKY gene family and underpinning the underlying regulatory roles of CsWRKY29 and CsWRKY37 from tea plants that conferred cold tolerance in transgenic Arabidopsis plants.
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Claims 8
Claim · model-written summary
The tea genome contains 86 CsWRKY genes.
Verbatim
e0“a total of 86 members of the CsWRKY genes were identified from the tea plant genome”
Claim · model-written summary
Most tea CsWRKY promoters contain stress-associated cis elements.
Verbatim
e0“Most of these genes contain several important Cis-regulatory elements in the promoter regions associated with multiple stress-responses.”
e1“tea plant genome”
Claim · model-written summary
Tea CsWRKY genes were classified into three groups with 21, 58, and 7 members.
Verbatim
e0“These genes were further classified into three groups, I, II, and III, each with 21, 58, and 7 members, respectively.”
e1“tea plant genome”
Claim · model-written summary
Tandem duplications likely drove tea CsWRKY amplification, unlike whole-genome duplication.
Verbatim
e0“tandem duplications, but not the whole genome duplication, are likely to drive the amplification of CsWRKY genes in tea plants”
Claim · model-written summary
All 86 CsWRKY genes showed differential expression across tissues or abiotic stress exposures.
Verbatim
e0“All the 86 CsWRKY genes showed differential expression patterns either in different tissues, or under exposure to diverse abiotic stresses such as drought, cold acclimation, and MeJA treatments.”
e1“tea plant genome”
Claim · model-written summary
Silencing CsWRKY29 and CsWRKY37 made tea plants susceptible to cold stress.
- Taxon
- tea plante0
- Treatment
- silencing of these genese0
- Gene
- CsWRKY29 and CsWRKY37e1
- Outcome
- phenotypes susceptible to cold stresse0
- Direction
- susceptiblee0
Not reportedComparator
Verbatim
e0“the silencing of these genes resulted in tea plant phenotypes susceptible to cold stress”
e1“two genes, CsWRKY29 and CsWRKY37”
Claim · model-written summary
Arabidopsis overexpressing CsWRKY29 or CsWRKY37 had higher survival under freezing than wild type.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- overexpressing CsWRKY29 and CsWRKY37 genese0
- Gene
- CsWRKY29 and CsWRKY37e0
- Outcome
- survival ratese0
- Direction
- highere0
- Comparator
- wild type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic Arabidopsis lines overexpressing CsWRKY29 and CsWRKY37 genes showed higher survival rates and lower malondialdehyde levels under freezing treatment than the wild type plants”
Claim · model-written summary
Arabidopsis overexpressing CsWRKY29 or CsWRKY37 had lower malondialdehyde under freezing than wild type.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- overexpressing CsWRKY29 and CsWRKY37 genese0
- Gene
- CsWRKY29 and CsWRKY37e0
- Outcome
- malondialdehyde levelse0
- Direction
- lowere0
- Comparator
- wild type plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic Arabidopsis lines overexpressing CsWRKY29 and CsWRKY37 genes showed higher survival rates and lower malondialdehyde levels under freezing treatment than the wild type plants”
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