Abstract
Non-coding RNAs play crucial roles in plant responses to viral stresses. However, their molecular mechanisms in tea leaf spot responses remain unclear. In this study, using Camellia sinensis, we identified lncRNA81246 as a long non-coding RNA that localizes to both the nucleus and cytoplasm. It functions as a competitive endogenous RNA, thereby disrupting CsNAC1 (encoding NAC domain-containing protein 1) degradation mediated by miR164d. Silencing lncRNA81246 increased the resistance of tea plants to presistanceathogens, whereas transient lncRNA81246-overexpression plants showed decreased resistance to pathogens. Co-expression assays in Nicotiana benthamiana revealed that lncRNA81246 affects the miR164d-CsNAC1 regulatory module. Transient miR164d-overexpression and silencing assays demonstrated its positive regulation of tea plant resistance. Specifically, silencing its target, CsNAC1, enhanced disease resistance, whereas transient overexpression reduced plant resistance. Yeast one-hybrid, dual-luciferase, and RT-qPCR assay results suggested that CsNAC1 alters the expression of CsEXLB1, whereas AsODN and tobacco transient overexpression assays showed that CsEXLB1 negatively regulated tea plant resistance. Thus, our research demonstrated that lncRNA81246 acts as a mediator to interfere with the miR164d-CsNAC1 regulatory module involved in the disease resistance of tea plants.
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Claims 10
Claim · model-written summary
lncRNA81246 localized to nucleus and cytoplasm.
- Taxon
- Camellia sinensis
Camellia sinensist0 - Gene
- lncRNA81246e0
- Outcome
- localizes to both the nucleus and cytoplasme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identified lncRNA81246 as a long non-coding RNA that localizes to both the nucleus and cytoplasm.”
t0“using Camellia sinensis”
Claim · model-written summary
lncRNA81246 disrupted miR164d-mediated CsNAC1 degradation.
- Taxon
- Camellia sinensisCamellia sinensist0
- Gene
- lncRNA81246e1
- Outcome
- CsNAC1 (encoding NAC domain-containing protein 1) degradatione0
- Qualifier
- mediated by miR164de0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It functions as a competitive endogenous RNA, thereby disrupting CsNAC1 (encoding NAC domain-containing protein 1) degradation mediated by miR164d.”
e1“we identified lncRNA81246 as a long non-coding RNA that localizes to both the nucleus and cytoplasm.”
t0“using Camellia sinensis”
Claim · model-written summary
Silencing lncRNA81246 increased tea resistance to pathogens.
- Taxon
- tea plantse0
- Treatment
- Silencing lncRNA81246e0
- Gene
- lncRNA81246e0
- Outcome
- resistance of tea plants to presistanceathogense0
- Direction
- increasede0
Not reportedComparator
Verbatim
e0“Silencing lncRNA81246 increased the resistance of tea plants to presistanceathogens, whereas transient lncRNA81246-overexpression plants showed decreased resistance to pathogens.”
Claim · model-written summary
Transient lncRNA81246 overexpression decreased tea resistance to pathogens.
- Taxon
- tea plantse0
- Treatment
- transient lncRNA81246-overexpressione0
- Gene
- lncRNA81246e0
- Outcome
- resistance to pathogense0
- Direction
- decreasede0
Not reportedComparator
Verbatim
e0“Silencing lncRNA81246 increased the resistance of tea plants to presistanceathogens, whereas transient lncRNA81246-overexpression plants showed decreased resistance to pathogens.”
Claim · model-written summary
In Nicotiana benthamiana co-expression assays lncRNA81246 affected the miR164d-CsNAC1 module.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Gene
- lncRNA81246e0
- Outcome
- miR164d-CsNAC1 regulatory modulee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Co-expression assays in Nicotiana benthamiana revealed that lncRNA81246 affects the miR164d-CsNAC1 regulatory module.”
Claim · model-written summary
miR164d positively regulated tea resistance in overexpression and silencing assays.
Verbatim
e0“Transient miR164d-overexpression and silencing assays demonstrated its positive regulation of tea plant resistance.”
Claim · model-written summary
CsNAC1 silencing enhanced disease resistance.
- Taxon
- teat0
- Treatment
- silencing its target, CsNAC1e0
- Gene
- CsNAC1e0
- Outcome
- disease resistancee0
- Direction
- enhancede0
Not reportedComparator
Verbatim
e0“Specifically, silencing its target, CsNAC1, enhanced disease resistance, whereas transient overexpression reduced plant resistance.”
t0“tea leaf spot”
Claim · model-written summary
CsNAC1 overexpression reduced plant resistance.
- Taxon
- teat0
- Treatment
- transient overexpressione0
- Gene
- CsNAC1e0
- Outcome
- plant resistancee0
- Direction
- reducede0
Not reportedComparator
Verbatim
e0“Specifically, silencing its target, CsNAC1, enhanced disease resistance, whereas transient overexpression reduced plant resistance.”
t0“tea leaf spot”
Claim · model-written summary
CsNAC1 altered CsEXLB1 expression.
Not reportedDirection
Verbatim
e0“Yeast one-hybrid, dual-luciferase, and RT-qPCR assay results suggested that CsNAC1 alters the expression of CsEXLB1, whereas AsODN and tobacco transient overexpression assays showed that CsEXLB1 negatively regulated tea plant resistance.”
Claim · model-written summary
CsEXLB1 negatively regulated tea resistance.
Verbatim
e0“Yeast one-hybrid, dual-luciferase, and RT-qPCR assay results suggested that CsNAC1 alters the expression of CsEXLB1, whereas AsODN and tobacco transient overexpression assays showed that CsEXLB1 negatively regulated tea plant resistance.”
The same questions in other papers
loss of function · disease · Camellia sinensis (black tea)Positive and negative results
4 papers in the same direction as this paper · 4 in the opposite direction
- negative
- negative
- negative
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See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.