Abstract
Drought stress severely limits growth and causes losses in the yield of tea plants. Exogenous application of 24-epibrassinolide (EBR) positively regulates drought responses in various plants. However, whether EBR could contribute to drought resistance in tea plants and the underlying mechanisms has not been investigated. Here, we found that EBR application is beneficial for the drought tolerance of tea plants. The transcriptome results revealed that EBR could contribute to tea plant drought resistance by promoting galactinol and abscisic acid (ABA) biosynthesis gene expression. The content of galactinol was elevated by EBR and EBR-responsive CsDof1.1 positively regulated the expression of the galactinol synthase genes CsGolS2-1 and CsGolS2-2 to contribute to the accumulation of galactinol by directly binding to their promoters. Moreover, exogenous EBR was found to elevate the expression of genes related to ABA signal transduction and stomatal closure regulation, which resulted in the promotion of stomatal closure. In addition, EBR-responsive CsMYC2-2 is involved in ABA accumulation by binding to the promoters CsNCED1 and CsNCED2 to activate their expression. In summary, findings in this study provide knowledge into the transcriptional regulatory mechanism of EBR-induced drought resistance in tea plants.
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Claims 9
Claim · model-written summary
EBR application benefited drought tolerance in tea plants.
Not reportedComparator
Verbatim
e0“EBR application is beneficial for the drought tolerance of tea plants”
Claim · model-written summary
EBR promoted expression of galactinol and ABA biosynthesis genes in tea plant drought resistance.
Verbatim
e0“EBR could contribute to tea plant drought resistance by promoting galactinol and abscisic acid (ABA) biosynthesis gene expression”
Claim · model-written summary
EBR elevated galactinol content in tea plants.
Not reportedComparator
Verbatim
e0“The content of galactinol was elevated by EBR”
t0“tea plants”
Claim · model-written summary
CsDof1.1 positively regulated CsGolS2-1 and CsGolS2-2 expression by binding their promoters, contributing to galactinol accumulation.
Verbatim
e0“EBR-responsive CsDof1.1 positively regulated the expression of the galactinol synthase genes CsGolS2-1 and CsGolS2-2 to contribute to the accumulation of galactinol by directly binding to their promoters”
t0“tea plants”
Claim · model-written summary
Exogenous EBR elevated expression of ABA signal transduction genes.
- Taxon
- tea plantst0
- Treatment
- exogenous EBRe0
- Outcome
- expression of genes related to ABA signal transductione0
- Direction
- elevatee0
Not reportedComparator
Verbatim
e0“exogenous EBR was found to elevate the expression of genes related to ABA signal transduction and stomatal closure regulation”
t0“tea plants”
Claim · model-written summary
Exogenous EBR elevated expression of stomatal closure regulation genes.
- Taxon
- tea plantst0
- Treatment
- exogenous EBRe0
- Outcome
- expression of genes related to ABA signal transduction and stomatal closure regulatione0
- Direction
- elevatee0
Not reportedComparator
Verbatim
e0“exogenous EBR was found to elevate the expression of genes related to ABA signal transduction and stomatal closure regulation”
t0“tea plants”
Claim · model-written summary
EBR-associated gene expression promoted stomatal closure.
Verbatim
e0“which resulted in the promotion of stomatal closure”
t0“tea plants”
Claim · model-written summary
CsMYC2-2 binds CsNCED1 and CsNCED2 promoters and activates their expression.
Verbatim
e0“EBR-responsive CsMYC2-2 is involved in ABA accumulation by binding to the promoters CsNCED1 and CsNCED2 to activate their expression”
t0“tea plants”
Claim · model-written summary
CsMYC2-2 is involved in ABA accumulation in tea plants.
Verbatim
e0“CsMYC2-2 is involved in ABA accumulation by binding to the promoters CsNCED1 and CsNCED2”
t0“tea plants”