Abstract
Isolating the genes responsible for cadmium (Cd) accumulation and tolerance in oilseed rape and uncovering their functional mechanism is of great significance for guiding genetic improvement to cope with heavy metal pollution. In this study, we screened the cDNA library of Brassica napus cv. Westar using a yeast genetic complementation system and isolated BnMT2-22a, BnMT2-22b and BnMT3b, which can mediate Cd tolerance in yeast. They all have two cysteine-rich domains in their sequence. Ectopic expression of these MTs demonstrated that all of them enhanced Cd and Cu tolerance in yeast, but had no effect on Mn and Zn tolerance. The fusion of the red fluorescent protein mRFP did not affect their function in mediating Cd tolerance, and using these functional fusion proteins we observed that they were all localized in cytosol. Meanwhile, their expression in yeast did not affect the accumulation of Cd in the yeast transformants. Gene expression analyses found that BnMT2-22a, BnMT2-22b and BnMT3b were all induced by Cd in roots, and BnMT3b was also significantly induced in shoots. These results indicate that the genes BnMT2-22a, BnMT2-22b and BnMT3b isolated with cDNA library screening can mediate Cd tolerance, and they may detoxify Cd via cytosolic chelation.
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Claims 10
Claim · model-written summary
BnMT2-22a, BnMT2-22b and BnMT3b mediated cadmium tolerance in yeast.
- Taxon
- Brassica napus
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napust0 - Gene
- BnMT2-22a, BnMT2-22b and BnMT3be0
- Outcome
- Cd tolerance in yeaste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“isolated BnMT2-22a, BnMT2-22b and BnMT3b, which can mediate Cd tolerance in yeast”
t0“Brassica napus cv. Westar”
Claim · model-written summary
The three isolated metallothioneins each had two cysteine-rich domains.
- Taxon
- Brassica napusBrassica napust0
- Gene
- BnMT2-22a, BnMT2-22b and BnMT3be1
- Outcome
- two cysteine-rich domains in their sequencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“They all have two cysteine-rich domains in their sequence”
t0“Brassica napus cv. Westar”
e1“isolated BnMT2-22a, BnMT2-22b and BnMT3b”
Claim · model-written summary
Ectopic expression of the metallothioneins enhanced Cd and Cu tolerance in yeast.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- Ectopic expression of these MTse1
- Outcome
- Cd and Cu tolerance in yeaste0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all of them enhanced Cd and Cu tolerance in yeast”
t0“Brassica napus cv. Westar”
e1“Ectopic expression of these MTs”
Claim · model-written summary
Ectopic expression of the metallothioneins did not affect Mn and Zn tolerance in yeast.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- Ectopic expression of these MTse1
- Outcome
- Mn and Zn tolerancee0
- Direction
- no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“had no effect on Mn and Zn tolerance”
t0“Brassica napus cv. Westar”
e1“Ectopic expression of these MTs”
Claim · model-written summary
mRFP fusion did not affect metallothionein-mediated Cd tolerance.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- fusion of the red fluorescent protein mRFPe0
- Gene
- mRFPe0
- Outcome
- function in mediating Cd tolerancee0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The fusion of the red fluorescent protein mRFP did not affect their function in mediating Cd tolerance”
t0“Brassica napus cv. Westar”
Claim · model-written summary
The functional fusion proteins localized in the cytosol.
- Taxon
- Brassica napusBrassica napust0
- Outcome
- localized in cytosole0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these functional fusion proteins we observed that they were all localized in cytosol”
t0“Brassica napus cv. Westar”
Claim · model-written summary
Metallothionein expression in yeast did not affect Cd accumulation.
- Taxon
- Brassica napusBrassica napust0
- Treatment
- expression in yeaste0
- Outcome
- accumulation of Cd in the yeast transformantse0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“their expression in yeast did not affect the accumulation of Cd in the yeast transformants”
t0“Brassica napus cv. Westar”
Claim · model-written summary
Cd induced expression of all three BnMT genes in roots.
- Taxon
- Brassica napusBrassica napust0
- Organ / stage
- rootse0
- Treatment
- Cde0
- Gene
- BnMT2-22a, BnMT2-22b and BnMT3be0
- Outcome
- BnMT2-22a, BnMT2-22b and BnMT3be0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BnMT2-22a, BnMT2-22b and BnMT3b were all induced by Cd in roots”
t0“Brassica napus cv. Westar”
Claim · model-written summary
Cd significantly induced BnMT3b in shoots.
- Taxon
- Brassica napusBrassica napust0
- Organ / stage
- shootse0
- Treatment
- Cde1
- Gene
- BnMT3be0
- Outcome
- BnMT3be0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BnMT3b was also significantly induced in shoots”
t0“Brassica napus cv. Westar”
e1“all induced by Cd in roots”
Claim · model-written summary
The authors propose cytosolic chelation as a possible Cd detoxification route.
- Taxon
- Brassica napusBrassica napust0
- Outcome
- detoxify Cde0
- Qualifier
- cytosolic chelatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“they may detoxify Cd via cytosolic chelation”
t0“Brassica napus cv. Westar”
The same questions in other papers
overexpression · disease · Brassica napus (oilseed rape)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.