Abstract
Identifying potential molecular tags for drought tolerance is essential for achieving higher crop productivity under drought stress. We employed an integrated genomics-assisted breeding and functional genomics strategy involving association mapping, fine mapping, map-based cloning, molecular haplotyping and transcript profiling in the introgression lines (ILs)- and near isogenic lines (NILs)-based association panel and mapping population of chickpea (Cicer arietinum). This combinatorial approach delineated a bHLH (basic helix-loop-helix) transcription factor, CabHLH10 (Cicer arietinum bHLH10) underlying a major QTL, along with its derived natural alleles/haplotypes governing yield traits under drought stress in chickpea. CabHLH10 binds to a cis-regulatory G-box promoter element to modulate the expression of RD22 (responsive to desiccation 22), a drought/abscisic acid (ABA)-responsive gene (via a trans-expression QTL), and two strong yield-enhancement photosynthetic efficiency (PE) genes. This, in turn, upregulates other downstream drought-responsive and ABA signaling genes, as well as yield-enhancing PE genes, thus increasing plant adaptation to drought with reduced yield penalty. We showed that a superior allele of CabHLH10 introgressed into the NILs improved root and shoot biomass and PE, thereby enhancing yield and productivity during drought without compromising agronomic performance. Furthermore, overexpression of CabHLH10 in chickpea and Arabidopsis (Arabidopsis thaliana) conferred enhanced drought tolerance by improving root and shoot agro-morphological traits. These findings facilitate translational genomics for crop improvement and the development of genetically tailored, climate-resilient, high-yielding chickpea cultivars.
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Claims 7
Claim · model-written summary
CabHLH10 underlies a major yield QTL and natural haplotypes under drought.
- Taxon
- chickpea
Cicer arietinume0 - Gene
- CabHLH10e0
- Outcome
- major QTLe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This combinatorial approach delineated a bHLH (basic helix-loop-helix) transcription factor, CabHLH10 (Cicer arietinum bHLH10) underlying a major QTL, along with its derived natural alleles/haplotypes governing yield traits under drought stress in chickpea.”
Claim · model-written summary
CabHLH10 binds a G-box to regulate RD22 and two photosynthetic efficiency genes.
- Taxon
- chickpeaCicer arietinumt0
- Gene
- CabHLH10e0
- Outcome
- expression of RD22 (responsive to desiccation 22)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CabHLH10 binds to a cis-regulatory G-box promoter element to modulate the expression of RD22 (responsive to desiccation 22), a drought/abscisic acid (ABA)-responsive gene (via a trans-expression QTL), and two strong yield-enhancement photosynthetic efficiency (PE) genes.”
t0“chickpea”
Claim · model-written summary
CabHLH10 upregulates downstream drought and yield associated genes.
- Taxon
- chickpeaCicer arietinumt0
- Gene
- drought-responsive and ABA signaling genese0
- Outcome
- plant adaptation to drought with reduced yield penaltye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This, in turn, upregulates other downstream drought-responsive and ABA signaling genes, as well as yield-enhancing PE genes, thus increasing plant adaptation to drought with reduced yield penalty.”
t0“chickpea”
Claim · model-written summary
The superior CabHLH10 allele improved root and shoot biomass and photosynthetic efficiency.
- Taxon
- chickpeaCicer arietinumt0
- Treatment
- a superior allele of CabHLH10 introgressed into the NILse0
- Gene
- CabHLH10e0
- Outcome
- root and shoot biomass and PEe0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a superior allele of CabHLH10 introgressed into the NILs improved root and shoot biomass and PE”
t0“chickpea”
Claim · model-written summary
The superior CabHLH10 allele enhanced drought yield without compromising agronomic performance.
- Taxon
- chickpeaCicer arietinumt0
- Treatment
- a superior allele of CabHLH10 introgressed into the NILse1
- Outcome
- yield and productivitye0
- Direction
- enhancinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“thereby enhancing yield and productivity during drought without compromising agronomic performance.”
t0“chickpea”
e1“a superior allele of CabHLH10 introgressed into the NILs improved root and shoot biomass and PE”
Claim · model-written summary
CabHLH10 overexpression improved drought tolerance in chickpea.
- Taxon
- chickpeaCicer arietinume0
- Treatment
- overexpression of CabHLH10e0
- Gene
- CabHLH10e0
- Outcome
- drought tolerancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of CabHLH10 in chickpea and Arabidopsis (Arabidopsis thaliana) conferred enhanced drought tolerance by improving root and shoot agro-morphological traits.”
Claim · model-written summary
CabHLH10 overexpression improved drought tolerance in Arabidopsis.
- Taxon
- Arabidopsis (Arabidopsis thaliana)
Arabidopsis thalianae0 - Treatment
- overexpression of CabHLH10e0
- Gene
- CabHLH10e0
- Outcome
- drought tolerancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of CabHLH10 in chickpea and Arabidopsis (Arabidopsis thaliana) conferred enhanced drought tolerance by improving root and shoot agro-morphological traits.”
The same questions in other papers
transgenic · yield · Cicer arietinum (chickpea)Positive and negative results
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