Abstract
Root hairs play crucial roles in the roots, including nutrient uptake, water assimilation, and anchorage with soil, along with supporting rhizospheric microorganisms. In rice, ammonia uptake is mediated by a specialized ammonium transporter (AMT). AMT1;1, AMT1;2, and AMT1;3 have been extensively studied in relation to nitrogen signaling. Cellulose synthase-like D1 (CSLD1) is essential for cell expansion and is highly specific to root hair cells. csld1 mutants showed successful initiation but failed to elongate. However, when nitrogen was depleted, csld1 root hairs resumed elongation. Further experiments revealed that in the presence of ammonium (NH4+), csld1 roots failed to elongate. csld1 elongated normally in the presence of nitrate (NO3−). Expression analysis showed an increase in root hair-specific AMT1;2 expression in csld1. CSLD1 was positively co-expressed with AMT1;2 changing nitrogen concentration in the growth media. CSLD1 showed increased expression in the presence of both ammonium and nitrate. Methylammonium (MeA) treatment of CSLD1 overexpression lines suggests that CSLD1 does not directly participate in nitrogen transport. Further studies on the root hair elongation mutant sndp1 showed that nitrogen assimilation is unlikely to depend on root hair length. Therefore, these results suggest that CSLD1 is closely involved in nitrogen-dependent root hair elongation and regulation of AMT1;2 expression in rice roots.
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Claims 9
Claim · model-written summary
csld1 root hairs initiated but failed to elongate.
- Taxon
- Rice
Oryza sativat0 - Material
- csld1 mutantse0
- Organ / stage
- Root Hairt1
- Outcome
- successful initiation but failed to elongatee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“csld1 mutants showed successful initiation but failed to elongate.”
t0“Rice”
t1“Root Hair”
Claim · model-written summary
With nitrogen depleted, csld1 root hairs resumed elongation.
- Taxon
- RiceOryza sativat0
- Material
- csld1e0
- Treatment
- nitrogen was depletede0
- Outcome
- root hairs resumed elongatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when nitrogen was depleted, csld1 root hairs resumed elongation.”
t0“Rice”
Claim · model-written summary
In ammonium, csld1 roots failed to elongate.
- Taxon
- RiceOryza sativat0
- Material
- csld1e0
- Treatment
- ammonium (NH4+)e0
- Outcome
- roots failed to elongatee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the presence of ammonium (NH4+), csld1 roots failed to elongate.”
t0“Rice”
Claim · model-written summary
In nitrate, csld1 elongated normally.
- Taxon
- RiceOryza sativat0
- Material
- csld1e0
- Treatment
- nitrate (NO3−)e0
- Outcome
- elongated normallye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“csld1 elongated normally in the presence of nitrate (NO3−).”
t0“Rice”
Claim · model-written summary
Root hair AMT1;2 expression increased in csld1.
- Taxon
- RiceOryza sativat0
- Material
- csld1e0
- Gene
- AMT1;2e0
- Outcome
- root hair-specific AMT1;2 expressione0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an increase in root hair-specific AMT1;2 expression in csld1.”
t0“Rice”
Claim · model-written summary
CSLD1 and AMT1;2 were positively co-expressed as media nitrogen concentration changed.
- Taxon
- RiceOryza sativat0
- Treatment
- nitrogen concentratione0
- Gene
- CSLD1e0
- Outcome
- co-expressed with AMT1;2e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CSLD1 was positively co-expressed with AMT1;2 changing nitrogen concentration in the growth media.”
t0“Rice”
Claim · model-written summary
Both ammonium and nitrate increased CSLD1 expression.
- Taxon
- RiceOryza sativat0
- Treatment
- both ammonium and nitratee0
- Gene
- CSLD1e0
- Outcome
- expressione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CSLD1 showed increased expression in the presence of both ammonium and nitrate.”
t0“Rice”
Claim · model-written summary
Methylammonium response suggested CSLD1 does not directly participate in nitrogen transport.
- Taxon
- RiceOryza sativat0
- Treatment
- Methylammonium (MeA) treatmente0
- Gene
- CSLD1e0
- Outcome
- does not directly participate in nitrogen transporte0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Methylammonium (MeA) treatment of CSLD1 overexpression lines suggests that CSLD1 does not directly participate in nitrogen transport.”
t0“Rice”
Claim · model-written summary
sndp1 mutant results suggested nitrogen assimilation is unlikely to depend on root hair length.
- Taxon
- RiceOryza sativat0
- Material
- sndp1e0
- Organ / stage
- root haire0
- Outcome
- nitrogen assimilation is unlikely to depend on root hair lengthe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“root hair elongation mutant sndp1 showed that nitrogen assimilation is unlikely to depend on root hair length.”
t0“Rice”
The same questions in other papers
nitrogen · growth · Oryza sativa (rice)Positive and negative results
401 papers in the same direction as this paper · 101 in the opposite direction · 8 reporting no difference from an alternative
- negative
- negative
- negative
507 papers more in this group
See all 511 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.