Abstract
The use of cold plasma in the treatment of seeds before sowing presents a promising technique for sustainable agriculture. The objective of this study is to evaluate the effect of cold plasma treatment on the morphology of wheat seeds (Triticum aestivum L. ‘Dacic’ and ‘Otilia’), their germination, biochemical composition, and the nutritional quality of wheat grass. Wheat seeds were morphologically and elementally characterized by atomic force microscopy (AFM), scanning electron microscopy coupled with energy dispersive X-ray spectroscopy (SEM-EDX), X-ray computer tomography (CT), and particle-induced X-ray emission (PIXE). Helium was used as a working gas for plasma generation and the analysis of the species produced showed the presence of NOγ, OH, N2 and N2+ and O. Evaluation of germination and plant growth for 10 days (wheat grass stage) highlighted a specific trend for each cultivar. The biochemical analysis of wheat grass highlighted an increase in the chlorophyll content in the plasma-treated variants, an increase in the flavonoid and polyphenol content in ‘Dacic’-treated variant, while the soluble protein content, antioxidant activity, and color were not affected. The analysis of the nutritional quality of wheat grass by the FT-NIR analytical technique highlighted an increase in the ash content in the plasma-treated wheat cultivars, while the humidity, proteins, neutral detergent fiber (NDF), acid detergent fiber (ADF), and energy values were not affected.
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Claims 8
Claim · model-written summary
Germination and plant growth over 10 days followed a specific trend for each wheat cultivar under plasma treatment.
- Taxon
- Triticum aestivum L.
Triticum aestivumt0 - Outcome
- germination and plant growthe1
- Qualifier
- specific trend for each cultivare1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Triticum aestivum L.”
e1“Evaluation of germination and plant growth for 10 days (wheat grass stage) highlighted a specific trend for each cultivar”
Claim · model-written summary
Chlorophyll content of wheat grass increased in plasma-treated variants.
- Taxon
- Triticum aestivum L.
Triticum aestivumt0 - Organ / stage
- wheat grasse2
- Treatment
- cold plasma treatmente0
- Outcome
- chlorophyll contente2
- Direction
- increasee2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Triticum aestivum L.”
e0“the effect of cold plasma treatment on the morphology of wheat seeds”
e2“The biochemical analysis of wheat grass highlighted an increase in the chlorophyll content in the plasma-treated variants”
Claim · model-written summary
Flavonoid and polyphenol content of wheat grass increased in the Dacic plasma-treated variant.
- Taxon
- Triticum aestivum L.
Triticum aestivumt0 - Material
- ‘Dacic’e3
- Treatment
- cold plasma treatmente0
- Outcome
- flavonoid and polyphenol contente3
- Direction
- increasee3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Triticum aestivum L.”
e0“the effect of cold plasma treatment on the morphology of wheat seeds”
e3“an increase in the flavonoid and polyphenol content in ‘Dacic’-treated variant, while the soluble protein content, antioxidant activity, and color were not affected”
Claim · model-written summary
Soluble protein content, antioxidant activity and colour of wheat grass were not affected by plasma treatment.
- Taxon
- Triticum aestivum L.
Triticum aestivumt0 - Treatment
- cold plasma treatmente0
- Outcome
- soluble protein content, antioxidant activity, and colore4
- Direction
- no_changeinferrede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Triticum aestivum L.”
e0“the effect of cold plasma treatment on the morphology of wheat seeds”
e4“the soluble protein content, antioxidant activity, and color were not affected”
Claim · model-written summary
Ash content of wheat grass increased in plasma-treated cultivars.
- Taxon
- Triticum aestivum L.
Triticum aestivumt0 - Treatment
- cold plasma treatmente0
- Outcome
- ash contente5
- Direction
- increasee5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Triticum aestivum L.”
e0“the effect of cold plasma treatment on the morphology of wheat seeds”
e5“an increase in the ash content in the plasma-treated wheat cultivars”
Claim · model-written summary
Humidity, protein and neutral detergent fiber of wheat grass were not affected by plasma treatment.
- Taxon
- Triticum aestivum L.
Triticum aestivumt0 - Treatment
- cold plasma treatmente0
- Outcome
- humidity, proteins, neutral detergent fiber (NDF)e6
- Direction
- no_changeinferrede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Triticum aestivum L.”
e0“the effect of cold plasma treatment on the morphology of wheat seeds”
e6“the humidity, proteins, neutral detergent fiber (NDF), acid detergent fiber (ADF), and energy values were not affected”
Claim · model-written summary
Acid detergent fiber and energy value of wheat grass were not affected by plasma treatment.
- Taxon
- Triticum aestivum L.
Triticum aestivumt0 - Treatment
- cold plasma treatmente0
- Outcome
- acid detergent fiber (ADF), and energy valuese6
- Direction
- no_changeinferrede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Triticum aestivum L.”
e0“the effect of cold plasma treatment on the morphology of wheat seeds”
e6“the humidity, proteins, neutral detergent fiber (NDF), acid detergent fiber (ADF), and energy values were not affected”
Claim · model-written summary
Helium plasma produced NOγ, OH, N2, N2+ and O species.
- Taxon
- Triticum aestivum L.
Triticum aestivumt0 - Outcome
- the presence of NOγ, OH, N2 and N2+ and Oe7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
t0“Triticum aestivum L.”
e7“the analysis of the species produced showed the presence of NOγ, OH, N2 and N2+ and O”
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