Abstract
Recent findings suggest that cold acclimation can enhance cold resistance in wheat. Dongnongdongmai 1 (DM1) is a winter wheat variety that can overwinter at −30 °C; however, its cold acclimation mechanism is yet to be fully elucidated. Here, we elucidated the potential mechanisms of cold acclimation in DM1 and the China Spring (CS) variety, especially the role of the antioxidant system, using transcriptome and physiological analyses. Cold stress increased H2O2 and O2− production in both varieties; however, CS had higher contents of H2O2 and O2− than DM1. Moreover, cold significantly increased ROS-scavenging activities in DM1, especially at 30 days after exposure. Gene ontology (GO) analysis showed that differentially expressed peroxidase (POD) genes were enriched in antioxidant activity, with most POD genes being significantly upregulated in DM1 under cold acclimation. Additionally, cold acclimation increased the expression of cold acclimation protein (CAP), late embryogenesis abundant protein (LEA), and cold-responsive genes in both varieties, with higher expression levels in DM1. Overall, the results showed that DM1 exhibited a higher cold tolerance than CS during cold acclimation by increasing the expression of POD genes, LEA, CAP, and cold-responsive proteins, improving the understanding of the mechanism of cold resistance in DM1.
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Claims 8
Claim · model-written summary
Cold stress increased H2O2 and O2− production in both wheat varieties.
- Taxon
- wheat
Triticum aestivumt0 - Treatment
- Cold stresse0
- Outcome
- H2O2 and O2− productione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Winter and Spring Wheat”
e0“Cold stress increased H2O2 and O2− production in both varieties”
Claim · model-written summary
CS had higher H2O2 and O2− contents than DM1.
- Taxon
- wheatTriticum aestivumt0
- Material
- CSe0
- Outcome
- contents of H2O2 and O2−e0
- Direction
- highere0
- Comparator
- DM1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Winter and Spring Wheat”
e0“CS had higher contents of H2O2 and O2− than DM1”
Claim · model-written summary
Cold significantly increased ROS-scavenging activities in DM1, particularly at day 30.
- Taxon
- wheatTriticum aestivumt0
- Material
- DM1e0
- Treatment
- colde0
- Outcome
- ROS-scavenging activitiese0
- Direction
- increasede0
- Qualifier
- 30 days after exposuree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Winter and Spring Wheat”
e0“cold significantly increased ROS-scavenging activities in DM1, especially at 30 days after exposure”
Claim · model-written summary
Differentially expressed POD genes were enriched in antioxidant activity.
- Taxon
- wheatTriticum aestivumt0
- Gene
- peroxidase (POD) genese0
- Outcome
- enriched in antioxidant activitye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Winter and Spring Wheat”
e0“differentially expressed peroxidase (POD) genes were enriched in antioxidant activity”
Claim · model-written summary
Most POD genes were significantly upregulated in DM1 during cold acclimation.
- Taxon
- wheatTriticum aestivumt0
- Material
- DM1e0
- Treatment
- cold acclimatione0
- Gene
- POD genese0
- Outcome
- POD genese0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Winter and Spring Wheat”
e0“most POD genes being significantly upregulated in DM1 under cold acclimation”
Claim · model-written summary
Cold acclimation increased CAP, LEA and cold-responsive gene expression in both varieties.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- cold acclimatione0
- Outcome
- expression of cold acclimation protein (CAP), late embryogenesis abundant protein (LEA), and cold-responsive genese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Winter and Spring Wheat”
e0“cold acclimation increased the expression of cold acclimation protein (CAP), late embryogenesis abundant protein (LEA), and cold-responsive genes in both varieties”
Claim · model-written summary
Expression of CAP, LEA and cold-responsive genes was higher in DM1.
- Taxon
- wheatTriticum aestivumt0
- Material
- DM1e0
- Outcome
- expression levelse0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Winter and Spring Wheat”
e0“higher expression levels in DM1”
Claim · model-written summary
DM1 showed higher cold tolerance than CS during acclimation.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Winter and Spring Wheat”
e0“DM1 exhibited a higher cold tolerance than CS during cold acclimation”
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See all 17 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.