Abstract
Waterlogging caused by short and severe, or prolonged precipitation can be attributed to global warming. Pumpkin plants are drought-tolerant but not tolerate to waterlogging stress. Under frequent rain and waterlogging conditions, the production of pumpkins is of lower quality, sometimes rotten, and harvest failure occurs in severe cases. Therefore, it is of great significance to assess the waterlogging tolerance mechanism of pumpkin plants. In this study, 10 novel pumpkin varieties from Baimi series were used. The waterlogging tolerance level of pumpkin plants was evaluated by measuring waterlogging tolerance coefficient of biomass and physiological indices using waterlogging stress simulation method. The criteria to evaluate the waterlogging tolerance capacities of pumpkin plants were also explored. Using principal component and membership function analysis, waterlogging tolerance levels of the pumpkin varieties were ranked as follows: Baimi No. 10>; Baimi No. 5>; Baimi No. 1>; Baimi No. 2>; Baimi No. 3>; Baimi No. 7>; Baimi No. 9>; Baimi No. 6>; Baimi No. 4>; Baimi No. 8. Based on the results, Baimi No. 10 was identified with strong waterlogging tolerance and Baimi No. 8 with weak waterlogging tolerance. The responses of malondialdehyde (MDA), proline, key enzymes responsible for anaerobic respiration, and antioxidant enzymes to waterlogging stress were studied in pumpkin plants. The relative expression levels of related genes were determined using real-time fluorescence quantitative PCR technique. The aim of our study was to assess the waterlogging tolerance mechanism of pumpkin plants, thus laying a theoretical foundation for breeding waterlogging-tolerant varieties in the future. After flooding stress treatment, the antioxidant enzyme activities, contents of proline and alcohol dehydrogenases of Baimi No. 10 and Baimi No. 8 displayed an increase followed by a decrease. All indices of Baimi No. 10 were higher than Baimi No. 8. MDA contents gradually increased, with the content being higher in Baimi No. 8 than Baimi No. 10. The activities of pyruvate decarboxylases (PDCs) in Baimi No. 8 and Baimi No. 10 exhibited a decrease initially, followed by an increase, and then a decrease again. The PDC activity in Baimi No. 8 was generally higher than Baimi No. 10. The relative expression levels of genes encoding superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase were consistent with their corresponding enzyme activities. During the early stage of flooding stress, pumpkin plants waterlogging tolerance was improved by enhancing the expression levels of antioxidant enzyme encoding genes and increasing the antioxidant enzyme activities.
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Claims 14
Claim · model-written summary
Pumpkin varieties were ranked for waterlogging tolerance from Baimi No. 10 to Baimi No. 8.
Verbatim
e0“waterlogging tolerance levels of the pumpkin varieties were ranked as follows: Baimi No. 10>; Baimi No. 5>; Baimi No. 1>; Baimi No. 2>; Baimi No. 3>; Baimi No. 7>; Baimi No. 9>; Baimi No. 6>; Baimi No. 4>; Baimi No. 8.”
Claim · model-written summary
Baimi No. 10 showed strong waterlogging tolerance.
Verbatim
e0“Baimi No. 10 was identified with strong waterlogging tolerance and Baimi No. 8 with weak waterlogging tolerance.”
t0“pumpkin”
Claim · model-written summary
Baimi No. 8 showed weak waterlogging tolerance.
Verbatim
e0“Baimi No. 8 with weak waterlogging tolerance.”
t0“pumpkin”
Claim · model-written summary
Flooding caused antioxidant enzyme activities, proline contents and alcohol dehydrogenases in Baimi No. 10 to increase and then decrease.
- Taxon
- pumpkint0
- Material
- Baimi No. 10e0
- Treatment
- flooding stress treatmente0
- Outcome
- antioxidant enzyme activities, contents of proline and alcohol dehydrogenasese0
- Direction
- an increase followed by a decreasee0
Not reportedComparator
Verbatim
e0“After flooding stress treatment, the antioxidant enzyme activities, contents of proline and alcohol dehydrogenases of Baimi No. 10 and Baimi No. 8 displayed an increase followed by a decrease.”
t0“pumpkin”
Claim · model-written summary
Flooding caused antioxidant enzyme activities, proline contents and alcohol dehydrogenases in Baimi No. 8 to increase and then decrease.
- Taxon
- pumpkint0
- Material
- Baimi No. 8e0
- Treatment
- flooding stress treatmente0
- Outcome
- antioxidant enzyme activities, contents of proline and alcohol dehydrogenasese0
- Direction
- an increase followed by a decreasee0
Not reportedComparator
Verbatim
e0“After flooding stress treatment, the antioxidant enzyme activities, contents of proline and alcohol dehydrogenases of Baimi No. 10 and Baimi No. 8 displayed an increase followed by a decrease.”
t0“pumpkin”
Claim · model-written summary
These indices were higher in Baimi No. 10 than Baimi No. 8.
Verbatim
e0“All indices of Baimi No. 10 were higher than Baimi No. 8.”
t0“pumpkin”
Claim · model-written summary
MDA contents gradually increased.
Verbatim
e0“MDA contents gradually increased, with the content being higher in Baimi No. 8 than Baimi No. 10.”
t0“pumpkin”
Claim · model-written summary
MDA content was higher in Baimi No. 8 than Baimi No. 10.
Verbatim
e0“MDA contents gradually increased, with the content being higher in Baimi No. 8 than Baimi No. 10.”
t0“pumpkin”
Claim · model-written summary
Baimi No. 8 PDC activities decreased, increased, and decreased again.
Verbatim
e0“The activities of pyruvate decarboxylases (PDCs) in Baimi No. 8 and Baimi No. 10 exhibited a decrease initially, followed by an increase, and then a decrease again.”
t0“pumpkin”
Claim · model-written summary
Baimi No. 10 PDC activities decreased, increased, and decreased again.
Verbatim
e0“The activities of pyruvate decarboxylases (PDCs) in Baimi No. 8 and Baimi No. 10 exhibited a decrease initially, followed by an increase, and then a decrease again.”
t0“pumpkin”
Claim · model-written summary
PDC activity was generally higher in Baimi No. 8 than Baimi No. 10.
Verbatim
e0“The PDC activity in Baimi No. 8 was generally higher than Baimi No. 10.”
t0“pumpkin”
Claim · model-written summary
Superoxide dismutase, peroxidase and catalase gene expression tracked corresponding enzyme activities.
- Taxon
- pumpkint0
- Gene
- superoxide dismutase, peroxidase, catalasee0
- Outcome
- relative expression levelse0
- Qualifier
- consistent with their corresponding enzyme activitiese0
Not reportedDirection
Verbatim
e0“The relative expression levels of genes encoding superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase were consistent with their corresponding enzyme activities.”
t0“pumpkin”
Claim · model-written summary
Ascorbate peroxidase gene expression tracked corresponding enzyme activity.
- Taxon
- pumpkint0
- Gene
- ascorbate peroxidasee0
- Outcome
- relative expression levelse0
- Qualifier
- consistent with their corresponding enzyme activitiese0
Not reportedDirection
Verbatim
e0“The relative expression levels of genes encoding superoxide dismutase, peroxidase, catalase, and ascorbate peroxidase were consistent with their corresponding enzyme activities.”
t0“pumpkin”
Claim · model-written summary
Early flooding tolerance improved through enhanced antioxidant enzyme gene expression and activities.
Verbatim
e0“During the early stage of flooding stress, pumpkin plants waterlogging tolerance was improved by enhancing the expression levels of antioxidant enzyme encoding genes and increasing the antioxidant enzyme activities.”