Abstract
Selenium (Se) plays an important role in the growth of plants. Alfalfa (Medicago sativa L.) is a perennial legume forage crop with high nutritional value and Se-rich functions. Many studies have shown that selenium can promote alfalfa growth, but few have explored the molecular biology mechanisms behind this effect. In this study, alfalfa was divided into two groups. One group was sprayed with sodium selenite (Na2SeO3) and the other group was sprayed with distilled water as a control. This study determined the growth, reproductive traits, physiological changes, transcriptome and metabolome of both groups of alfalfa. We found that foliar spraying of 100 mg/L Na2SeO3 could significantly increase the growth rate, dry weight, total Se content, amount of pollen per flower, pollen viability, pod spirals, and seed number per pod of alfalfa plants. The level of chlorophyll, soluble protein, proline, and glutathione also increased dramatically in Na2SeO3-sprayed alfalfa seedlings. After transcriptome and metabolome analysis, a total of 614 differentially expressed genes (DEGs) and 1500 differentially expressed metabolites (DEMs), including 26 secondary differentially metabolites were identified. The DEGs were mainly enriched in MAPK signaling pathway, phenylpropanoid biosynthesis, isoflavonoid biosynthesis, cutin, suberine, and wax biosynthesis, and glycerolipid metabolism. The DEMs were mainly enriched in flavone and flavonol biosynthesis, carbon metabolism, glyoxylate and dicarboxylate metabolism, nitrogen metabolism, and phenylpropanoid biosynthesis. Integrative analysis of transcriptome and metabolome showed that the foliar spraying of Na2SeO3 mainly affects phenylpropanoid biosynthesis to promote alfalfa growth.
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Claims 12
Claim · model-written summary
Sodium selenite increased alfalfa growth rate and dry weight.
- Taxon
- alfalfa
Medicago sativae0 - Treatment
- foliar spraying of 100 mg/L Na2SeO3e0
- Outcome
- growth rate, dry weighte0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“foliar spraying of 100 mg/L Na2SeO3 could significantly increase the growth rate, dry weight, total Se content, amount of pollen per flower, pollen viability, pod spirals, and seed number per pod of alfalfa plants”
Claim · model-written summary
Sodium selenite significantly increased total Se content.
- Taxon
- alfalfaMedicago sativae0
- Treatment
- foliar spraying of 100 mg/L Na2SeO3e0
- Outcome
- total Se contente0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“foliar spraying of 100 mg/L Na2SeO3 could significantly increase the growth rate, dry weight, total Se content, amount of pollen per flower, pollen viability, pod spirals, and seed number per pod of alfalfa plants”
Claim · model-written summary
Sodium selenite significantly increased pollen amount and viability.
- Taxon
- alfalfaMedicago sativae0
- Treatment
- foliar spraying of 100 mg/L Na2SeO3e0
- Outcome
- amount of pollen per flower, pollen viabilitye0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“foliar spraying of 100 mg/L Na2SeO3 could significantly increase the growth rate, dry weight, total Se content, amount of pollen per flower, pollen viability, pod spirals, and seed number per pod of alfalfa plants”
Claim · model-written summary
Sodium selenite significantly increased pod spirals and seeds per pod.
- Taxon
- alfalfaMedicago sativae0
- Treatment
- foliar spraying of 100 mg/L Na2SeO3e0
- Outcome
- pod spirals, and seed number per pode0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“foliar spraying of 100 mg/L Na2SeO3 could significantly increase the growth rate, dry weight, total Se content, amount of pollen per flower, pollen viability, pod spirals, and seed number per pod of alfalfa plants”
Claim · model-written summary
Sodium selenite increased chlorophyll and soluble protein in seedlings.
- Taxon
- alfalfaMedicago sativae0
- Organ / stage
- seedlingse0
- Treatment
- Na2SeO3-sprayede0
- Outcome
- chlorophyll, soluble proteine0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The level of chlorophyll, soluble protein, proline, and glutathione also increased dramatically in Na2SeO3-sprayed alfalfa seedlings”
Claim · model-written summary
Sodium selenite increased proline and glutathione in seedlings.
- Taxon
- alfalfaMedicago sativae0
- Organ / stage
- seedlingse0
- Treatment
- Na2SeO3-sprayede0
- Outcome
- proline, and glutathionee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The level of chlorophyll, soluble protein, proline, and glutathione also increased dramatically in Na2SeO3-sprayed alfalfa seedlings”
Claim · model-written summary
Transcriptome analysis identified 614 differentially expressed genes.
- Taxon
- alfalfaMedicago sativat0
- Outcome
- differentially expressed genes (DEGs)e0
- Value
- 614e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a total of 614 differentially expressed genes (DEGs) and 1500 differentially expressed metabolites (DEMs), including 26 secondary differentially metabolites were identified”
t0“Alfalfa”
Claim · model-written summary
Metabolome analysis identified 1500 differentially expressed metabolites.
- Taxon
- alfalfaMedicago sativat0
- Outcome
- differentially expressed metabolites (DEMs)e0
- Value
- 1500e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a total of 614 differentially expressed genes (DEGs) and 1500 differentially expressed metabolites (DEMs), including 26 secondary differentially metabolites were identified”
t0“Alfalfa”
Claim · model-written summary
Among the metabolites, 26 secondary differential metabolites were identified.
- Taxon
- alfalfaMedicago sativat0
- Outcome
- secondary differentially metabolitese0
- Value
- 26e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“including 26 secondary differentially metabolites were identified”
t0“Alfalfa”
Claim · model-written summary
Differentially expressed genes were enriched in several signaling, biosynthesis and metabolism pathways.
- Taxon
- alfalfaMedicago sativat0
- Outcome
- enriched in MAPK signaling pathway, phenylpropanoid biosynthesis, isoflavonoid biosynthesis, cutin, suberine, and wax biosynthesis, and glycerolipid metabolisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The DEGs were mainly enriched in MAPK signaling pathway, phenylpropanoid biosynthesis, isoflavonoid biosynthesis, cutin, suberine, and wax biosynthesis, and glycerolipid metabolism”
t0“Alfalfa”
Claim · model-written summary
Differentially expressed metabolites were enriched in flavonoid, carbon, nitrogen and phenylpropanoid pathways.
- Taxon
- alfalfaMedicago sativat0
- Outcome
- enriched in flavone and flavonol biosynthesis, carbon metabolism, glyoxylate and dicarboxylate metabolism, nitrogen metabolism, and phenylpropanoid biosynthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The DEMs were mainly enriched in flavone and flavonol biosynthesis, carbon metabolism, glyoxylate and dicarboxylate metabolism, nitrogen metabolism, and phenylpropanoid biosynthesis”
t0“Alfalfa”
Claim · model-written summary
The authors infer Na2SeO3 spraying promotes alfalfa growth mainly through phenylpropanoid biosynthesis.
- Taxon
- alfalfaMedicago sativae0
- Treatment
- foliar spraying of Na2SeO3e0
- Outcome
- promote alfalfa growthe0
- Qualifier
- phenylpropanoid biosynthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“foliar spraying of Na2SeO3 mainly affects phenylpropanoid biosynthesis to promote alfalfa growth”
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- positive
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- negative
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See all 38 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.