Paper · 2023 · Journal of Dairy Science

The effects of feeding α-amylase-enhanced corn silage with different dietary starch concentrations to lactating dairy cows on milk production, nutrient digestibility, and blood metabolites

K.C. Krogstad; B.J. Bradford

14
claims
31
verbatim quotes
20
citations
1
research areas
✓
Open access · cc-by

Abstract

Corn silage is one of the most common ingredients fed to dairy cattle. Advancement of corn silage genetics has improved nutrient digestibility and dairy cow lactation performance in the past. A corn silage hybrid with enhanced endogenous α-amylase activity (Enogen, Syngenta Seeds LLC) may improve milk production efficiency and nutrient digestibility when fed to lactating dairy cows. Furthermore, evaluating how Enogen silage interacts with different dietary starch content is important because the ruminal environment is influenced by the amount of rumen fermentable organic matter consumed. To evaluate the effects of Enogen corn silage and dietary starch content, we conducted an 8-wk randomized complete block experiment (2-wk covariate period, 6-wk experimental period) with a 2 × 2 factorial treatment arrangement using 44 cows (n = 11/treatment; 28 multiparous, 16 primiparous; 151 ± 42 d in milk; 668 ± 63.6 kg of body weight). Treatment factors were Enogen corn silage (ENO) or control (CON) corn silage included at 40% of diet dry matter and 25% (LO) or 30% (HI) dietary starch. Corn silage used in CON treatment was a similar hybrid as in ENO but without enhanced α-amylase activity. The experimental period began 41 d after silage harvest. Feed intake and milk yield data were collected daily, plasma metabolites and fecal pH were measured weekly, and digestibility was measured during the first and final weeks of the experimental period. Data were analyzed using a linear mixed model approach with repeated measures for all variables except for body condition score change and body weight change. Corn silage, starch, week, and their interactions were included as fixed effects; baseline covariates and their interactions with corn silage and starch were also tested. Block and cow served as the random effects. Plasma glucose, insulin, haptoglobin, and serum amyloid A concentrations were unaffected by treatment. Fecal pH was greater for cows fed ENO versus CON. Dry matter, crude protein, neutral detergent fiber, and starch digestibility were all greater for ENO than CON during wk 1, but differences were less by wk 6. The HI treatments depressed neutral detergent fiber digestibility compared with LO. Dry matter intake (DMI) was not affected by corn silage but was affected by the interaction of starch and week; in wk 1, DMI was similar but by wk 6, cows fed HI had 1.8 ± 0.93 kg/d less DMI than LO cows. Milk, energy-corrected milk, and milk protein yields were 1.7 ± 0.94 kg/d, 1.3 ± 0.70 kg/d, and 65 ± 27 g/d greater for HI than LO, respectively. In conclusion, ENO increased digestibility but it did not affect milk yield, component yields, or DMI. Increasing dietary starch content improved milk production and feed efficiency without affecting markers of inflammation or metabolism.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 14

Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Plasma glucose, insulin and haptoglobin concentrations were unaffected by corn silage or starch treatment.

Treatment
Enogen corn silage (ENO)e1
Outcome
Plasma glucose, insulin, haptoglobine2
Direction
unaffectede2
Comparator
control (CON) corn silagee1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Corn silage is one of the most common ingredients fed to dairy cattle”

✓ located verbatim in the source · abstract, characters 0–69 · show in text ↑
e1

“Treatment factors were Enogen corn silage (ENO) or control (CON) corn silage”

✓ located verbatim in the source · abstract, characters 938–1014 · show in text ↑
e2

“Plasma glucose, insulin, haptoglobin, and serum amyloid A concentrations were unaffected by treatment”

✓ located verbatim in the source · abstract, characters 1828–1929 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Serum amyloid A concentration was unaffected by corn silage or starch treatment.

Treatment
Enogen corn silage (ENO)e1
Outcome
serum amyloid Ae2
Direction
unaffectede2
Comparator
control (CON) corn silagee1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Corn silage is one of the most common ingredients fed to dairy cattle”

✓ located verbatim in the source · abstract, characters 0–69 · show in text ↑
e1

“Treatment factors were Enogen corn silage (ENO) or control (CON) corn silage”

✓ located verbatim in the source · abstract, characters 938–1014 · show in text ↑
e2

“Plasma glucose, insulin, haptoglobin, and serum amyloid A concentrations were unaffected by treatment”

✓ located verbatim in the source · abstract, characters 1828–1929 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Fecal pH was greater for cows fed ENO corn silage than CON.

Treatment
ENOe1
Outcome
Fecal pHe1
Direction
greatere1
Comparator
CONe1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Corn silage is one of the most common ingredients fed to dairy cattle”

✓ located verbatim in the source · abstract, characters 0–69 · show in text ↑
e1

“Fecal pH was greater for cows fed ENO versus CON”

✓ located verbatim in the source · abstract, characters 1931–1979 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Dry matter, crude protein and neutral detergent fiber digestibility were greater for ENO than CON in week 1; differences were smaller by week 6.

Treatment
ENOe1
Outcome
Dry matter, crude protein, neutral detergent fibere1
Direction
greatere1
Qualifier
during wk 1, but differences were less by wk 6e1
Comparator
CONe1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Corn silage is one of the most common ingredients fed to dairy cattle”

✓ located verbatim in the source · abstract, characters 0–69 · show in text ↑
e1

“Dry matter, crude protein, neutral detergent fiber, and starch digestibility were all greater for ENO than CON during wk 1, but differences were less by wk 6”

✓ located verbatim in the source · abstract, characters 1981–2138 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Starch digestibility was greater for ENO than CON in week 1; differences were smaller by week 6.

Treatment
ENOe1
Outcome
starch digestibilitye1
Direction
greatere1
Qualifier
during wk 1, but differences were less by wk 6e1
Comparator
CONe1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Corn silage is one of the most common ingredients fed to dairy cattle”

✓ located verbatim in the source · abstract, characters 0–69 · show in text ↑
e1

“Dry matter, crude protein, neutral detergent fiber, and starch digestibility were all greater for ENO than CON during wk 1, but differences were less by wk 6”

✓ located verbatim in the source · abstract, characters 1981–2138 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

The HI starch diet depressed neutral detergent fiber digestibility compared with LO.

Treatment
HIe1
Outcome
neutral detergent fiber digestibilitye1
Direction
depressede1
Comparator
LOe1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Corn silage is one of the most common ingredients fed to dairy cattle”

✓ located verbatim in the source · abstract, characters 0–69 · show in text ↑
e1

“The HI treatments depressed neutral detergent fiber digestibility compared with LO”

✓ located verbatim in the source · abstract, characters 2140–2222 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Dry matter intake was not affected by corn silage type.

Treatment
corn silagee1
Outcome
Dry matter intake (DMI)e1
Direction
not affectede1
Qualifier
but was affected by the interaction of starch and weeke1
Comparator
control (CON) corn silagee2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Corn silage is one of the most common ingredients fed to dairy cattle”

✓ located verbatim in the source · abstract, characters 0–69 · show in text ↑
e1

“Dry matter intake (DMI) was not affected by corn silage but was affected by the interaction of starch and week”

✓ located verbatim in the source · abstract, characters 2224–2334 · show in text ↑
e2

“Treatment factors were Enogen corn silage (ENO) or control (CON) corn silage”

✓ located verbatim in the source · abstract, characters 938–1014 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

By week 6, cows fed the HI starch diet had 1.8 kg/d lower dry matter intake than LO cows.

Treatment
HIe1
Outcome
DMIe1
Direction
lesse1
Value
1.8 ± 0.93 kg/de1
Qualifier
by wk 6e1
Comparator
LOe1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Corn silage is one of the most common ingredients fed to dairy cattle”

✓ located verbatim in the source · abstract, characters 0–69 · show in text ↑
e1

“in wk 1, DMI was similar but by wk 6, cows fed HI had 1.8 ± 0.93 kg/d less DMI than LO cows”

✓ located verbatim in the source · abstract, characters 2336–2427 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Dry matter intake was similar for HI and LO starch diets in week 1.

Treatment
HIe1
Outcome
DMIe1
Direction
similare1
Qualifier
in wk 1e1
Comparator
LOe1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Corn silage is one of the most common ingredients fed to dairy cattle”

✓ located verbatim in the source · abstract, characters 0–69 · show in text ↑
e1

“in wk 1, DMI was similar but by wk 6, cows fed HI had 1.8 ± 0.93 kg/d less DMI than LO cows”

✓ located verbatim in the source · abstract, characters 2336–2427 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Milk yield was 1.7 ± 0.94 kg/d greater for the HI than the LO starch diet.

Treatment
HIe1
Outcome
Milke1
Direction
greatere1
Value
1.7 ± 0.94 kg/de1
Comparator
LOe1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Corn silage is one of the most common ingredients fed to dairy cattle”

✓ located verbatim in the source · abstract, characters 0–69 · show in text ↑
e1

“Milk, energy-corrected milk, and milk protein yields were 1.7 ± 0.94 kg/d, 1.3 ± 0.70 kg/d, and 65 ± 27 g/d greater for HI than LO, respectively”

✓ located verbatim in the source · abstract, characters 2429–2573 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Energy-corrected milk was 1.3 ± 0.70 kg/d greater for the HI than the LO starch diet.

Treatment
HIe1
Outcome
energy-corrected milke1
Direction
greatere1
Value
1.3 ± 0.70 kg/de1
Comparator
LOe1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Corn silage is one of the most common ingredients fed to dairy cattle”

✓ located verbatim in the source · abstract, characters 0–69 · show in text ↑
e1

“Milk, energy-corrected milk, and milk protein yields were 1.7 ± 0.94 kg/d, 1.3 ± 0.70 kg/d, and 65 ± 27 g/d greater for HI than LO, respectively”

✓ located verbatim in the source · abstract, characters 2429–2573 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Milk protein yield was 65 ± 27 g/d greater for the HI than the LO starch diet.

Treatment
HIe1
Outcome
milk protein yieldse1
Direction
greatere1
Value
65 ± 27 g/de1
Comparator
LOe1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Corn silage is one of the most common ingredients fed to dairy cattle”

✓ located verbatim in the source · abstract, characters 0–69 · show in text ↑
e1

“Milk, energy-corrected milk, and milk protein yields were 1.7 ± 0.94 kg/d, 1.3 ± 0.70 kg/d, and 65 ± 27 g/d greater for HI than LO, respectively”

✓ located verbatim in the source · abstract, characters 2429–2573 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

ENO corn silage did not affect milk yield or component yields, although it increased digestibility.

Treatment
ENOe1
Outcome
milk yield, component yieldse1
Direction
did not affecte1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Corn silage is one of the most common ingredients fed to dairy cattle”

✓ located verbatim in the source · abstract, characters 0–69 · show in text ↑
e1

“ENO increased digestibility but it did not affect milk yield, component yields, or DMI”

✓ located verbatim in the source · abstract, characters 2590–2676 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Increasing dietary starch improved feed efficiency.

Treatment
Increasing dietary starch contente1
Outcome
feed efficiencye1
Direction
improvede1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Corn silage is one of the most common ingredients fed to dairy cattle”

✓ located verbatim in the source · abstract, characters 0–69 · show in text ↑
e1

“Increasing dietary starch content improved milk production and feed efficiency without affecting markers of inflammation or metabolism”

✓ located verbatim in the source · abstract, characters 2678–2812 · show in text ↑

Zea mays subsp. mays · yield · Zea mays (maize)Results differ

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processing · molecular: metabolites · Zea mays (maize)Positive and negative results

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8 of 10 groups shown: named compounds and organisms first, then groups whose results differ (checked claim by claim), then those with positive and negative results, then the smaller groups.

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.