Paper · 1981 · Canadian Journal of Animal Science

EFFECT OF ADDITIVES CONTAINING LACTIC ACID BACTERIA AND/OR HYDROLYTIC ENZYMES WITH AN ANTIOXIDANT UPON THE PRESERVATION OF CORN OR ALFALFA SILAGE

J. G. BUCHANAN-SMITH; Y. T. YAO

13
claims
25
verbatim quotes
29
citations
1
research areas
—
Not open access

Abstract

A silage additive containing lactic acid bacteria was tested for its effect upon preservation of corn silage, 35–40% dry matter (DM), in four pairs of upright silos (100-t capacity). The additive did not affect (P > 0.05) final pH, lactic or acetic acid concentration, crude protein or NPN-N and NH 4 + -N expressed as a percent of total N. Recovery of energy from treated silage was greater than control in the two pairs of silos where this was determined, but recoveries of dry matter and crude protein were not consistently affected. Two silage additives, containing hydrolytic enzymes and an antioxidant with or without lactic acid bacteria, were tested on alfalfa, 20, 30, 36.5 and 47.3% DM, in 250-mL laboratory silos. The experimental design used a4 (DM level) × 4 (added glucose — 0,4,8 and 12%, DM basis) × 3 (additive — control, a hydrolytic enzyme/antioxidant additive (E/AO), and E/AO plus lactic acid bacteria (E/AO +)) factorial arrangement of treatments. Alfalfa was harvested using farm equipment. For 60-day silage, the additives as either a primary factor in the design or in second-order interactions with either silage DM or glucose showed no effect (P > 0.05) upon pH, lactic acid, acetic acid, butyric acid, Fleig score, residual water-soluble carbohydrate and percent of total N as NPN-N or ammonia-N. Poor quality silage resulted from the fermentation of alfalfa at 20 and 30% DM, and although additional glucose resolved this problem the additives did not. Beneficial effects of additional water-soluble carbohydrate in alfalfa silage fermentation are evident in data presented. Thus an additive containing an antioxidant and hydrolytic enzymes was not very effective and numbers of lactic acid bacteria in untreated alfalfa harvested with farm equipment must already be sufficient for adequate fermentation.

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The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 13

Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

The lactic acid bacteria additive did not affect final pH, lactic or acetic acid concentration of corn silage.

Treatment
silage additive containing lactic acid bacteriae1
Outcome
final pH, lactic or acetic acid concentratione2
Direction
did not affecte2
Setting
upright silose1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“A silage additive containing lactic acid bacteria was tested for its effect upon preservation of corn silage, 35–40% dry matter (DM), in four pairs of upright silos (100-t capacity)”

✓ located verbatim in the source · abstract, characters 0–181 · show in text ↑
e2

“The additive did not affect (P > 0.05) final pH, lactic or acetic acid concentration, crude protein or NPN-N and NH 4 + -N expressed as a percent of total N”

✓ located verbatim in the source · abstract, characters 183–339 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

The lactic acid bacteria additive did not affect crude protein, NPN-N or NH4+-N of corn silage.

Treatment
silage additive containing lactic acid bacteriae1
Outcome
crude protein or NPN-N and NH 4 + -Ne2
Direction
did not affecte2
Setting
upright silose1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“A silage additive containing lactic acid bacteria was tested for its effect upon preservation of corn silage, 35–40% dry matter (DM), in four pairs of upright silos (100-t capacity)”

✓ located verbatim in the source · abstract, characters 0–181 · show in text ↑
e2

“The additive did not affect (P > 0.05) final pH, lactic or acetic acid concentration, crude protein or NPN-N and NH 4 + -N expressed as a percent of total N”

✓ located verbatim in the source · abstract, characters 183–339 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Recovery of energy from corn silage treated with the lactic acid bacteria additive was greater than control in two pairs of silos.

Treatment
silage additive containing lactic acid bacteriae1
Outcome
Recovery of energye3
Direction
greatere3
Comparator
controle3
Setting
two pairs of silose3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“A silage additive containing lactic acid bacteria was tested for its effect upon preservation of corn silage, 35–40% dry matter (DM), in four pairs of upright silos (100-t capacity)”

✓ located verbatim in the source · abstract, characters 0–181 · show in text ↑
e3

“Recovery of energy from treated silage was greater than control in the two pairs of silos where this was determined”

✓ located verbatim in the source · abstract, characters 341–456 · show in text ↑
Intervention effectobservedoriginal resultmixedmeasured

Claim · model-written summary

Recoveries of dry matter and crude protein in corn silage were not consistently affected by the lactic acid bacteria additive.

Treatment
silage additive containing lactic acid bacteriae1
Outcome
recoveries of dry matter and crude proteine4
Direction
not consistently affectede4

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays subsp. mays
Zea mays subsp. mays · TaxonDominicus Johannes Bergsma · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e1

“A silage additive containing lactic acid bacteria was tested for its effect upon preservation of corn silage, 35–40% dry matter (DM), in four pairs of upright silos (100-t capacity)”

✓ located verbatim in the source · abstract, characters 0–181 · show in text ↑
e4

“but recoveries of dry matter and crude protein were not consistently affected”

✓ located verbatim in the source · abstract, characters 458–535 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

The enzyme and antioxidant additive did not affect pH, lactic acid or acetic acid of 60-day alfalfa silage.

Taxon
alfalfaMedicago sativae5
Treatment
a hydrolytic enzyme/antioxidant additive (E/AO)e6
Outcome
pH, lactic acid, acetic acide7
Direction
no effecte7
Comparator
controle6
Setting
250-mL laboratory silose5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e5

“Two silage additives, containing hydrolytic enzymes and an antioxidant with or without lactic acid bacteria, were tested on alfalfa, 20, 30, 36.5 and 47.3% DM, in 250-mL laboratory silos”

✓ located verbatim in the source · abstract, characters 537–723 · show in text ↑
e6

“additive — control, a hydrolytic enzyme/antioxidant additive (E/AO)”

✓ located verbatim in the source · abstract, characters 819–886 · show in text ↑
e7

“For 60-day silage, the additives as either a primary factor in the design or in second-order interactions with either silage DM or glucose showed no effect (P > 0.05) upon pH, lactic acid, acetic acid, butyric acid, Fleig score, residual water-soluble carbohydrate and percent of total N as NPN-N or ammonia-N”

✓ located verbatim in the source · abstract, characters 1014–1323 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

The enzyme and antioxidant additive did not affect butyric acid, Fleig score or residual water-soluble carbohydrate of 60-day alfalfa silage.

Taxon
alfalfaMedicago sativae5
Treatment
a hydrolytic enzyme/antioxidant additive (E/AO)e6
Outcome
butyric acid, Fleig score, residual water-soluble carbohydratee7
Direction
no effecte7
Comparator
controle6
Setting
250-mL laboratory silose5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e5

“Two silage additives, containing hydrolytic enzymes and an antioxidant with or without lactic acid bacteria, were tested on alfalfa, 20, 30, 36.5 and 47.3% DM, in 250-mL laboratory silos”

✓ located verbatim in the source · abstract, characters 537–723 · show in text ↑
e6

“additive — control, a hydrolytic enzyme/antioxidant additive (E/AO)”

✓ located verbatim in the source · abstract, characters 819–886 · show in text ↑
e7

“For 60-day silage, the additives as either a primary factor in the design or in second-order interactions with either silage DM or glucose showed no effect (P > 0.05) upon pH, lactic acid, acetic acid, butyric acid, Fleig score, residual water-soluble carbohydrate and percent of total N as NPN-N or ammonia-N”

✓ located verbatim in the source · abstract, characters 1014–1323 · show in text ↑
Intervention effectobservedoriginal resultno effectnot significant (as stated)measured

Claim · model-written summary

The enzyme and antioxidant additive did not affect percent of total N as NPN-N or ammonia-N of 60-day alfalfa silage.

Taxon
alfalfaMedicago sativae5
Treatment
a hydrolytic enzyme/antioxidant additive (E/AO)e6
Outcome
percent of total N as NPN-N or ammonia-Ne7
Direction
no effecte7
Comparator
controle6
Setting
250-mL laboratory silose5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e5

“Two silage additives, containing hydrolytic enzymes and an antioxidant with or without lactic acid bacteria, were tested on alfalfa, 20, 30, 36.5 and 47.3% DM, in 250-mL laboratory silos”

✓ located verbatim in the source · abstract, characters 537–723 · show in text ↑
e6

“additive — control, a hydrolytic enzyme/antioxidant additive (E/AO)”

✓ located verbatim in the source · abstract, characters 819–886 · show in text ↑
e7

“For 60-day silage, the additives as either a primary factor in the design or in second-order interactions with either silage DM or glucose showed no effect (P > 0.05) upon pH, lactic acid, acetic acid, butyric acid, Fleig score, residual water-soluble carbohydrate and percent of total N as NPN-N or ammonia-N”

✓ located verbatim in the source · abstract, characters 1014–1323 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Poor quality alfalfa silage resulted from fermentation at 20 and 30% dry matter.

Taxon
alfalfaMedicago sativae0
Outcome
Poor quality silagee0
Qualifier
at 20 and 30% DMe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“Poor quality silage resulted from the fermentation of alfalfa at 20 and 30% DM, and although additional glucose resolved this problem the additives did not”

✓ located verbatim in the source · abstract, characters 1325–1480 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Additional glucose resolved the poor quality of alfalfa silage made at 20 and 30% dry matter.

Taxon
alfalfaMedicago sativae0
Treatment
additional glucosee0
Outcome
Poor quality silagee0
Direction
resolvede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“Poor quality silage resulted from the fermentation of alfalfa at 20 and 30% DM, and although additional glucose resolved this problem the additives did not”

✓ located verbatim in the source · abstract, characters 1325–1480 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

The additives did not resolve the poor quality of alfalfa silage made at 20 and 30% dry matter.

Taxon
alfalfaMedicago sativae0
Treatment
the additivese0
Outcome
Poor quality silagee0
Direction
did note0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“Poor quality silage resulted from the fermentation of alfalfa at 20 and 30% DM, and although additional glucose resolved this problem the additives did not”

✓ located verbatim in the source · abstract, characters 1325–1480 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Additional water-soluble carbohydrate had beneficial effects on alfalfa silage fermentation.

Taxon
alfalfaMedicago sativae0
Treatment
additional water-soluble carbohydratee0
Outcome
silage fermentatione0
Direction
Beneficial effectse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“Beneficial effects of additional water-soluble carbohydrate in alfalfa silage fermentation are evident in data presented”

✓ located verbatim in the source · abstract, characters 1482–1602 · show in text ↑
Intervention effectauthor interpretationoriginal resultnegativemeasured

Claim · model-written summary

The enzyme and antioxidant additive with lactic acid bacteria was not very effective on alfalfa silage.

Taxon
alfalfaMedicago sativae5
Treatment
an additive containing an antioxidant and hydrolytic enzymese6
Outcome
effectivee6
Direction
not verye6

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e5

“Two silage additives, containing hydrolytic enzymes and an antioxidant with or without lactic acid bacteria, were tested on alfalfa, 20, 30, 36.5 and 47.3% DM, in 250-mL laboratory silos”

✓ located verbatim in the source · abstract, characters 537–723 · show in text ↑
e6

“an additive containing an antioxidant and hydrolytic enzymes was not very effective”

✓ located verbatim in the source · abstract, characters 1609–1692 · show in text ↑
Mechanismauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

Lactic acid bacteria already present in untreated alfalfa are sufficient for adequate fermentation.

Taxon
alfalfaMedicago sativae7
Outcome
adequate fermentatione7
Qualifier
numbers of lactic acid bacteria in untreated alfalfa harvested with farm equipment must already be sufficiente7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Medicago sativa
Medicago sativa · TaxonDrepanostoma · CC BY 4.0 · iNaturalist ↗

Verbatim

e5

“Two silage additives, containing hydrolytic enzymes and an antioxidant with or without lactic acid bacteria, were tested on alfalfa, 20, 30, 36.5 and 47.3% DM, in 250-mL laboratory silos”

✓ located verbatim in the source · abstract, characters 537–723 · show in text ↑
e7

“numbers of lactic acid bacteria in untreated alfalfa harvested with farm equipment must already be sufficient for adequate fermentation”

✓ located verbatim in the source · abstract, characters 1697–1832 · show in text ↑

glucose · quality: general · Medicago sativa (alfalfa)Results agree

2 papers: 2 positive, 0 negative or no effect

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  1. positive
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71 papers: 62 positive, 9 negative or no effect

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.