Abstract
Volatile compounds including terpenes, aldehyde, phenol, and alcohol are significantly contributed floral and fruity aromas to the Muscat variety. 'Ruidu Hongyu' grapevine is one of the newly developed grape varieties, and cultivation of this variety has been extended across China due to unique quality traits and taste. In this study, HS-SPME/GC-MS and transcriptome sequencing analysis were performed to evaluate the impact of exogenous 2,4-epibrassinolide (EBR), jasmonic acid (JA), and their signaling inhibitors brassinazole (Brz)/sodium diethyldithiocarbamate (DIECA) on the biosynthesis of aroma substances in 'Ruidu Hongyu' grapevine. According to the results, exogenous BR and JA promoted the accumulation of various aroma substances, including hexenal, 2-hexenal, nerol oxide, vanillin, hotrienol, terpineol, neral, nerol, geraniol, and geranic acid. After EBR and JA treatments, most of the genes responsible for terpene, aldehyde, and alcohol biosynthesis expressed at a higher level than the CK group. Relatively, EBR treatment could not only promote endogenous BR biosynthesis and metabolism but also elevate BR signaling transduction. JA treatment contributed to endogenous JA and MeJA accumulation, as well. Through transcriptome sequencing, a total of 3043, 903, 1470, and 607 DEGs were identified in JA vs. JD, JA vs. CK, BR vs. CK, and BR vs. Brz, respectively. There were more DEGs under both EBR and JA treatments at late fruit ripening stages. The findings of this study increase our understanding regarding aroma substances biosynthesis and endogenous BR/JA metabolism in response to exogenous EBR and JA signals.
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Claims 12
Claim · model-written summary
Exogenous BR increased multiple aroma substances in Ruidu Hongyu berries.
- Material
- Ruidu Hongyut0
- Treatment
- exogenous BRe0
- Outcome
- accumulation of various aroma substancese0
- Direction
- promotede0
VitisNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous BR and JA promoted the accumulation of various aroma substances, including hexenal, 2-hexenal, nerol oxide, vanillin, hotrienol, terpineol, neral, nerol, geraniol, and geranic acid”
t0“Ruidu Hongyu’ grapevine berries”
Claim · model-written summary
Exogenous JA increased multiple aroma substances in Ruidu Hongyu berries.
- Material
- Ruidu Hongyut0
- Treatment
- JAe0
- Outcome
- accumulation of various aroma substancese0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“exogenous BR and JA promoted the accumulation of various aroma substances, including hexenal, 2-hexenal, nerol oxide, vanillin, hotrienol, terpineol, neral, nerol, geraniol, and geranic acid”
t0“Ruidu Hongyu’ grapevine berries”
Claim · model-written summary
EBR increased expression of most terpene, aldehyde and alcohol biosynthesis genes versus CK.
- Treatment
- EBRe0
- Outcome
- genes responsible for terpene, aldehyde, and alcohol biosynthesis expressede0
- Direction
- highere0
- Comparator
- CK groupe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After EBR and JA treatments, most of the genes responsible for terpene, aldehyde, and alcohol biosynthesis expressed at a higher level than the CK group”
t0“Ruidu Hongyu’ grapevine berries”
Claim · model-written summary
JA increased expression of most terpene, aldehyde and alcohol biosynthesis genes versus CK.
- Treatment
- JAe0
- Outcome
- genes responsible for terpene, aldehyde, and alcohol biosynthesis expressede0
- Direction
- highere0
- Comparator
- CK groupe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After EBR and JA treatments, most of the genes responsible for terpene, aldehyde, and alcohol biosynthesis expressed at a higher level than the CK group”
t0“Ruidu Hongyu’ grapevine berries”
Claim · model-written summary
EBR promoted endogenous BR biosynthesis and metabolism.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“EBR treatment could not only promote endogenous BR biosynthesis and metabolism but also elevate BR signaling transduction”
t0“Ruidu Hongyu’ grapevine berries”
Claim · model-written summary
EBR elevated BR signaling transduction.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“EBR treatment could not only promote endogenous BR biosynthesis and metabolism but also elevate BR signaling transduction”
t0“Ruidu Hongyu’ grapevine berries”
Claim · model-written summary
JA increased endogenous JA and MeJA accumulation.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“JA treatment contributed to endogenous JA and MeJA accumulation”
t0“Ruidu Hongyu’ grapevine berries”
Claim · model-written summary
Transcriptomics found 3043 DEGs for JA versus JD.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a total of 3043, 903, 1470, and 607 DEGs were identified in JA vs. JD, JA vs. CK, BR vs. CK, and BR vs. Brz, respectively”
t0“Ruidu Hongyu’ grapevine berries”
Claim · model-written summary
Transcriptomics found 903 DEGs for JA versus CK.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a total of 3043, 903, 1470, and 607 DEGs were identified in JA vs. JD, JA vs. CK, BR vs. CK, and BR vs. Brz, respectively”
t0“Ruidu Hongyu’ grapevine berries”
Claim · model-written summary
Transcriptomics found 1470 DEGs for BR versus CK.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a total of 3043, 903, 1470, and 607 DEGs were identified in JA vs. JD, JA vs. CK, BR vs. CK, and BR vs. Brz, respectively”
t0“Ruidu Hongyu’ grapevine berries”
Claim · model-written summary
Transcriptomics found 607 DEGs for BR versus Brz.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a total of 3043, 903, 1470, and 607 DEGs were identified in JA vs. JD, JA vs. CK, BR vs. CK, and BR vs. Brz, respectively”
t0“Ruidu Hongyu’ grapevine berries”
Claim · model-written summary
More DEGs occurred under EBR and JA at late ripening stages.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There were more DEGs under both EBR and JA treatments at late fruit ripening stages”
t0“Ruidu Hongyu’ grapevine berries”
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See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.