Abstract
Anthocyanins have important physiological functions and are beneficial to the improvement of fruit quality in strawberry. Light is important for anthocyanin biosynthesis, and specific light quality was identified to promote anthocyanin accumulation in many fruits. However, research on the molecular mechanisms of anthocyanin accumulation regulated by light quality in strawberry remains limited. Here we described the effects of red- and blue-light irradiation on anthocyanin accumulation in strawberry. The results showed that blue light, rather than red light, could lead to the rapid accumulation of anthocyanins after exposure to light for 48 hours. The transcriptional levels of anthocyanin structural and regulatory genes displayed similar trend to the anthocyanin content. To investigate the mechanism of blue light-induced anthocyanin accumulation, the homologs of Arabidopsis blue light signal transduction components, including the blue light photoreceptor FaCRY1 , an E3 ubiquitin ligase FaCOP1 and light-responsive factor FaHY5 , were cloned from the strawberry cultivar ‘Benihoppe’. The protein-protein interaction of FaCRY1-FaCOP1-FaHY5 was revealed by yeast two-hybrid and fluorescence signal assays. Functional complementation analysis showed that overexpression of either FaCOP1 or FaHY5 restored the anthocyanin content and hypocotyl length in corresponding Arabidopsis mutants under blue light. Moreover, dual-luciferase assays showed that FaHY5 could increase the activity of FaRAP (anthocyanin transport gene) promoter and that this function relied on other, likely B-box protein FaBBX22, factors. The overexpression of FaHY5 - VP16 (chimeric activator form of FaHY5) and FaBBX22 promoted the accumulation of anthocyanins in transgenic strawberry plants. Further, transcriptomic profiling indicated that the genes involved in the phenylpropanoid biosynthesis pathway were enriched in both FaHY5 - VP16- OX and FaBBX22 -OX strawberry plants. In summary, our findings provide insights into a mechanism involving the regulation of blue light-induced anthocyanin accumulation via a FaCRY1-FaCOP1-FaHY5 signal transduction module in strawberry.
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Claims 9
Claim · model-written summary
Blue light rapidly increased strawberry anthocyanins after 48 hours, unlike red light.
- Taxon
- strawberry
_single2.jpg?width=60)
Fragaria ×ananassat0 - Treatment
- blue lighte0
- Outcome
- accumulation of anthocyaninse0
- Direction
- rapid accumulatione0
- Qualifier
- after exposure to light for 48 hourse0
- Comparator
- red lighte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“blue light, rather than red light, could lead to the rapid accumulation of anthocyanins after exposure to light for 48 hours”
t0“strawberry”
Claim · model-written summary
Anthocyanin structural and regulatory gene transcription followed the anthocyanin content trend.
- Taxon
- strawberryFragaria ×ananassat0
- Gene
- anthocyanin structural and regulatory genese0
- Outcome
- transcriptional levelse0
- Comparator
- anthocyanin contente0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcriptional levels of anthocyanin structural and regulatory genes displayed similar trend to the anthocyanin content”
t0“strawberry”
Claim · model-written summary
FaCRY1, FaCOP1 and FaHY5 interacted as proteins.
- Taxon
- strawberryFragaria ×ananassat0
- Gene
- FaCRY1-FaCOP1-FaHY5e0
- Outcome
- protein-protein interactione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The protein-protein interaction of FaCRY1-FaCOP1-FaHY5 was revealed by yeast two-hybrid and fluorescence signal assays”
t0“strawberry”
Claim · model-written summary
FaCOP1 overexpression restored anthocyanin content and hypocotyl length in Arabidopsis mutants under blue light.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- overexpression of either FaCOP1 or FaHY5e0
- Gene
- FaCOP1e0
- Outcome
- anthocyanin content and hypocotyl lengthe0
- Direction
- restorede0
- Comparator
- corresponding Arabidopsis mutantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of either FaCOP1 or FaHY5 restored the anthocyanin content and hypocotyl length in corresponding Arabidopsis mutants under blue light”
Claim · model-written summary
FaHY5 overexpression restored anthocyanin content and hypocotyl length in Arabidopsis mutants under blue light.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- overexpression of either FaCOP1 or FaHY5e0
- Gene
- FaHY5e0
- Outcome
- anthocyanin content and hypocotyl lengthe0
- Direction
- restorede0
- Comparator
- corresponding Arabidopsis mutantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of either FaCOP1 or FaHY5 restored the anthocyanin content and hypocotyl length in corresponding Arabidopsis mutants under blue light”
Claim · model-written summary
FaHY5 increased FaRAP promoter activity, dependent on other factors likely including FaBBX22.
- Taxon
- strawberryFragaria ×ananassat0
- Gene
- FaHY5e0
- Outcome
- activity of FaRAP (anthocyanin transport gene) promotere0
- Direction
- increasee0
- Qualifier
- likely B-box protein FaBBX22e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FaHY5 could increase the activity of FaRAP (anthocyanin transport gene) promoter and that this function relied on other, likely B-box protein FaBBX22, factors”
t0“strawberry”
Claim · model-written summary
FaHY5-VP16 overexpression promoted anthocyanin accumulation in transgenic strawberry.
- Taxon
- strawberryFragaria ×ananassae0
- Treatment
- overexpression of FaHY5 - VP16e0
- Gene
- FaHY5 - VP16e0
- Outcome
- accumulation of anthocyaninse0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The overexpression of FaHY5 - VP16 (chimeric activator form of FaHY5) and FaBBX22 promoted the accumulation of anthocyanins in transgenic strawberry plants”
Claim · model-written summary
FaBBX22 overexpression promoted anthocyanin accumulation in transgenic strawberry.
- Taxon
- strawberryFragaria ×ananassae0
- Treatment
- FaBBX22e0
- Gene
- FaBBX22e0
- Outcome
- accumulation of anthocyaninse0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The overexpression of FaHY5 - VP16 (chimeric activator form of FaHY5) and FaBBX22 promoted the accumulation of anthocyanins in transgenic strawberry plants”
Claim · model-written summary
Phenylpropanoid biosynthesis genes were enriched in FaHY5-VP16-OX and FaBBX22-OX strawberry.
- Taxon
- strawberryFragaria ×ananassae0
- Material
- FaHY5 - VP16- OX and FaBBX22 -OXe0
- Gene
- genes involved in the phenylpropanoid biosynthesis pathwaye0
- Outcome
- enrichede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the genes involved in the phenylpropanoid biosynthesis pathway were enriched in both FaHY5 - VP16- OX and FaBBX22 -OX strawberry plants”
The same questions in other papers
light · quality: phytochemicals · Fragaria x ananassa (strawberry)Results differ
27 papers in the same direction as this paper · 4 in the opposite direction
- positive
- no effect
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