Abstract
GDSL esterases/lipases are a subclass of lipolytic enzymes that play critical roles in plant growth and development, stress response, and pathogen defense. However, the GDSL esterase/lipase genes involved in the pathogen response of apple remain to be identified and characterized. Thus, in this study, we aimed to analyze the phenotypic difference between the resistant variety, Fuji, and susceptible variety, Gala, during infection with C. gloeosporioides, screen for anti-disease-associated proteins in Fuji leaves, and elucidate the underlying mechanisms. The results showed that GDSL esterase/lipase protein GELP1 contributed to C. gloeosporioides infection defense in apple. During C. gloeosporioides infection, GELP1 expression was significantly upregulated in Fuji. Fuji leaves exhibited a highly resistant phenotype compared with Gala leaves. The formation of infection hyphae of C. gloeosporioides was inhibited in Fuji. Moreover, recombinant His:GELP1 protein suppressed hyphal formation during infection in vitro. Transient expression in Nicotiana benthamiana showed that GELP1-eGFP localized to the endoplasmic reticulum and chloroplasts. GELP1 overexpression in GL-3 plants increased resistance to C. gloeosporioides. MdWRKY15 expression was upregulated in the transgenic lines. Notably, GELP1 transcript levels were elevated in GL-3 after salicylic acid treatment. These results suggest that GELP1 increases apple resistance to C. gloeosporioides by indirectly regulating salicylic acid biosynthesis.
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Claims 10
Claim · model-written summary
GELP1 contributed to apple defense against C. gloeosporioides.
- Taxon
- apple
Malus domesticae0 - Interactor
- C. gloeosporioides
Colletotrichum gloeosporioidese0pathogen - Gene
- GELP1e0
- Outcome
- C. gloeosporioides infection defensee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GDSL esterase/lipase protein GELP1 contributed to C. gloeosporioides infection defense in apple.”
Claim · model-written summary
GELP1 expression rose significantly in infected Fuji.
- Taxon
- AppleMalus domesticat0
- Material
- Fujie0
- Interactor
- C. gloeosporioidesColletotrichum gloeosporioidese0pathogen
- Gene
- GELP1e0
- Outcome
- GELP1 expressione0
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“During C. gloeosporioides infection, GELP1 expression was significantly upregulated in Fuji.”
t0“GDSL Esterase/Lipase GELP1 Involved in the Defense of Apple Leaves against Colletotrichum gloeosporioides Infection”
Claim · model-written summary
Fuji leaves were more resistant than Gala leaves.
- Taxon
- AppleMalus domesticat0
- Material
- Fujie0
- Organ / stage
- leavese0
- Interactor
- C. gloeosporioidesColletotrichum gloeosporioidese1pathogen
- Outcome
- resistant phenotypee0
- Direction
- highly resistante0
- Comparator
- Gala leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fuji leaves exhibited a highly resistant phenotype compared with Gala leaves.”
e1“during infection with C. gloeosporioides”
t0“GDSL Esterase/Lipase GELP1 Involved in the Defense of Apple Leaves against Colletotrichum gloeosporioides Infection”
Claim · model-written summary
C. gloeosporioides infection hyphae were inhibited in Fuji.
- Taxon
- AppleMalus domesticat0
- Material
- Fujie0
- Interactor
- C. gloeosporioidesColletotrichum gloeosporioidese0pathogen
- Outcome
- formation of infection hyphae of C. gloeosporioidese0
- Direction
- inhibitede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The formation of infection hyphae of C. gloeosporioides was inhibited in Fuji.”
t0“GDSL Esterase/Lipase GELP1 Involved in the Defense of Apple Leaves against Colletotrichum gloeosporioides Infection”
Claim · model-written summary
Recombinant His:GELP1 suppressed infection hyphae formation in vitro.
- Taxon
- AppleMalus domesticat0
- Treatment
- recombinant His:GELP1 proteine0
- Interactor
- C. gloeosporioidesColletotrichum gloeosporioidese1pathogen
- Gene
- His:GELP1e0
- Outcome
- hyphal formatione0
- Direction
- suppressede0
- Setting
- in vitroe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“recombinant His:GELP1 protein suppressed hyphal formation during infection in vitro.”
e1“The formation of infection hyphae of C. gloeosporioides was inhibited in Fuji.”
t0“GDSL Esterase/Lipase GELP1 Involved in the Defense of Apple Leaves against Colletotrichum gloeosporioides Infection”
Claim · model-written summary
GELP1-eGFP localized to endoplasmic reticulum and chloroplasts in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Gene
- GELP1-eGFPe0
- Outcome
- localized to the endoplasmic reticulum and chloroplastse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient expression in Nicotiana benthamiana showed that GELP1-eGFP localized to the endoplasmic reticulum and chloroplasts.”
Claim · model-written summary
GELP1 overexpression increased GL-3 resistance to C. gloeosporioides.
- Taxon
- AppleMalus domesticat0
- Material
- GL-3e0
- Treatment
- GELP1 overexpressione0
- Interactor
- C. gloeosporioidesColletotrichum gloeosporioidese0pathogen
- Gene
- GELP1e0
- Outcome
- resistance to C. gloeosporioidese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GELP1 overexpression in GL-3 plants increased resistance to C. gloeosporioides.”
t0“GDSL Esterase/Lipase GELP1 Involved in the Defense of Apple Leaves against Colletotrichum gloeosporioides Infection”
Claim · model-written summary
MdWRKY15 expression increased in transgenic lines.
- Taxon
- AppleMalus domesticat0
- Material
- transgenic linese0
- Gene
- MdWRKY15e0
- Outcome
- MdWRKY15 expressione0
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MdWRKY15 expression was upregulated in the transgenic lines.”
t0“GDSL Esterase/Lipase GELP1 Involved in the Defense of Apple Leaves against Colletotrichum gloeosporioides Infection”
Claim · model-written summary
Salicylic acid treatment elevated GELP1 transcripts in GL-3.
- Taxon
- AppleMalus domesticat0
- Material
- GL-3e0
- Treatment
- salicylic acid treatmente0
- Gene
- GELP1e0
- Outcome
- GELP1 transcript levelse0
- Direction
- elevatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GELP1 transcript levels were elevated in GL-3 after salicylic acid treatment.”
t0“GDSL Esterase/Lipase GELP1 Involved in the Defense of Apple Leaves against Colletotrichum gloeosporioides Infection”
Claim · model-written summary
Authors propose GELP1 indirectly regulates salicylic acid biosynthesis to increase apple resistance.
- Taxon
- appleMalus domesticae0
- Interactor
- C. gloeosporioidesColletotrichum gloeosporioidese0pathogen
- Gene
- GELP1e0
- Compound
- salicylic acide0
- Outcome
- resistance to C. gloeosporioidese0
- Direction
- increasese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GELP1 increases apple resistance to C. gloeosporioides by indirectly regulating salicylic acid biosynthesis.”
The same questions in other papers
salicylic acid · molecular: expression · Malus domestica (apple)Results agree
7 papers in the same direction as this paper
- positive
- positive
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See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.