Paper · 2023 · Agronomy

Fermentation Quality and Chemical Composition of Industrial Hemp (Cannabis sativa L.) Silage Inoculated with Bacterial Starter Cultures—A Pilot Study

Barbara Wróbel; Marek Hryniewicz; Iryna Kulkova; et al.

8
claims
23
verbatim quotes
6
citations
1
research areas
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Open access · cc-by

Abstract

Industrial hemp (Cannabis sativa L.) is a plant species cultivated as a raw material for fiber extraction. Alternatively, hemp biomass can be used for feeding or energy purposes. This study was conducted to investigate the effect of inoculation with a lactic acid bacteria starter culture on the fermentation and chemical compositions of hemp silages. Hemp shoots (HS) and hemp flowers (HF) were ensiled in mini laboratory silos without or with the inoculation of the commercial starter culture Lactosil Biogaz (Lentilactobacillus buchnerii KKP 907 p; L. buchneri A KKP 2047 p; Pediococcus acidilactici KKP 2065 p). After 7 and 42 days of ensiling, the fermentation quality and chemical compositions of the silages were assessed. The use of Lactosil Biogas for hemp resulted in a decrease in pH, increase in lactic acid (LA), and reduction in fungal abundance in the HS silage. In the case of the HF silage, the bacterial inoculation was less effective; however, an increase in LA and a decrease in butyric acid (BA) were observed. As a result of the ensilage process, decreases in crude fiber and hemicellulose were observed in the HS and HF silages. Thus, hemp ensiling with biological additives is an effective pre-treatment of hemp plants for subsequent biofuel production that can preserve the biomass and provide the year-round availability of feedstock.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 8

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Lactosil Biogas inoculation decreased silage pH in hemp shoots relative to uninoculated silage.

Organ / stage
Hemp shoots (HS)e3
Treatment
Lactosil Biogase2
Interactor
lactic acid bacteria starter culturee1other
Outcome
pHe2
Direction
decreasee2
Comparator
withoute3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa subsp. sativa
Cannabis sativa subsp. sativa · TaxonNo machine-readable author provided. Bastique assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“the effect of inoculation with a lactic acid bacteria starter culture on the fermentation and chemical compositions of hemp silages”

✓ located verbatim in the source · abstract, characters 219–350 · show in text ↑
e2

“The use of Lactosil Biogas for hemp resulted in a decrease in pH, increase in lactic acid (LA), and reduction in fungal abundance in the HS silage”

✓ located verbatim in the source · abstract, characters 730–876 · show in text ↑
e3

“Hemp shoots (HS) and hemp flowers (HF) were ensiled in mini laboratory silos without or with the inoculation”

✓ located verbatim in the source · abstract, characters 352–460 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Lactosil Biogas inoculation increased lactic acid in hemp shoot silage relative to uninoculated silage.

Organ / stage
Hemp shoots (HS)e3
Treatment
Lactosil Biogase2
Interactor
lactic acid bacteria starter culturee1other
Outcome
lactic acid (LA)e2
Direction
increasee2
Comparator
withoute3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa subsp. sativa
Cannabis sativa subsp. sativa · TaxonNo machine-readable author provided. Bastique assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“the effect of inoculation with a lactic acid bacteria starter culture on the fermentation and chemical compositions of hemp silages”

✓ located verbatim in the source · abstract, characters 219–350 · show in text ↑
e2

“The use of Lactosil Biogas for hemp resulted in a decrease in pH, increase in lactic acid (LA), and reduction in fungal abundance in the HS silage”

✓ located verbatim in the source · abstract, characters 730–876 · show in text ↑
e3

“Hemp shoots (HS) and hemp flowers (HF) were ensiled in mini laboratory silos without or with the inoculation”

✓ located verbatim in the source · abstract, characters 352–460 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Lactosil Biogas inoculation reduced fungal abundance in hemp shoot silage relative to uninoculated silage.

Organ / stage
Hemp shoots (HS)e3
Treatment
Lactosil Biogase2
Interactor
lactic acid bacteria starter culturee1other
Outcome
fungal abundancee2
Direction
reductione2
Comparator
withoute3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa subsp. sativa
Cannabis sativa subsp. sativa · TaxonNo machine-readable author provided. Bastique assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“the effect of inoculation with a lactic acid bacteria starter culture on the fermentation and chemical compositions of hemp silages”

✓ located verbatim in the source · abstract, characters 219–350 · show in text ↑
e2

“The use of Lactosil Biogas for hemp resulted in a decrease in pH, increase in lactic acid (LA), and reduction in fungal abundance in the HS silage”

✓ located verbatim in the source · abstract, characters 730–876 · show in text ↑
e3

“Hemp shoots (HS) and hemp flowers (HF) were ensiled in mini laboratory silos without or with the inoculation”

✓ located verbatim in the source · abstract, characters 352–460 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Bacterial inoculation was less effective for hemp flower silage than for hemp shoot silage.

Organ / stage
HF silagee4
Treatment
bacterial inoculatione4
Outcome
effectivee4
Direction
lesse4
Comparator
withoute3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa subsp. sativa
Cannabis sativa subsp. sativa · TaxonNo machine-readable author provided. Bastique assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“the effect of inoculation with a lactic acid bacteria starter culture on the fermentation and chemical compositions of hemp silages”

✓ located verbatim in the source · abstract, characters 219–350 · show in text ↑
e3

“Hemp shoots (HS) and hemp flowers (HF) were ensiled in mini laboratory silos without or with the inoculation”

✓ located verbatim in the source · abstract, characters 352–460 · show in text ↑
e4

“In the case of the HF silage, the bacterial inoculation was less effective”

✓ located verbatim in the source · abstract, characters 878–952 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Bacterial inoculation increased lactic acid in hemp flower silage.

Organ / stage
HF silagee4
Treatment
bacterial inoculatione4
Outcome
LAe5
Direction
increasee5
Comparator
withoute3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa subsp. sativa
Cannabis sativa subsp. sativa · TaxonNo machine-readable author provided. Bastique assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“the effect of inoculation with a lactic acid bacteria starter culture on the fermentation and chemical compositions of hemp silages”

✓ located verbatim in the source · abstract, characters 219–350 · show in text ↑
e3

“Hemp shoots (HS) and hemp flowers (HF) were ensiled in mini laboratory silos without or with the inoculation”

✓ located verbatim in the source · abstract, characters 352–460 · show in text ↑
e4

“In the case of the HF silage, the bacterial inoculation was less effective”

✓ located verbatim in the source · abstract, characters 878–952 · show in text ↑
e5

“an increase in LA and a decrease in butyric acid (BA) were observed”

✓ located verbatim in the source · abstract, characters 963–1030 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Bacterial inoculation decreased butyric acid in hemp flower silage.

Organ / stage
HF silagee4
Treatment
bacterial inoculatione4
Outcome
butyric acid (BA)e5
Direction
decreasee5
Comparator
withoute3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa subsp. sativa
Cannabis sativa subsp. sativa · TaxonNo machine-readable author provided. Bastique assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“the effect of inoculation with a lactic acid bacteria starter culture on the fermentation and chemical compositions of hemp silages”

✓ located verbatim in the source · abstract, characters 219–350 · show in text ↑
e3

“Hemp shoots (HS) and hemp flowers (HF) were ensiled in mini laboratory silos without or with the inoculation”

✓ located verbatim in the source · abstract, characters 352–460 · show in text ↑
e4

“In the case of the HF silage, the bacterial inoculation was less effective”

✓ located verbatim in the source · abstract, characters 878–952 · show in text ↑
e5

“an increase in LA and a decrease in butyric acid (BA) were observed”

✓ located verbatim in the source · abstract, characters 963–1030 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Ensilage decreased crude fiber and hemicellulose in hemp shoot and flower silages.

Organ / stage
HS and HF silagese6
Treatment
ensilage processe6
Outcome
crude fiber and hemicellulosee6
Direction
decreasese6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa subsp. sativa
Cannabis sativa subsp. sativa · TaxonNo machine-readable author provided. Bastique assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“the effect of inoculation with a lactic acid bacteria starter culture on the fermentation and chemical compositions of hemp silages”

✓ located verbatim in the source · abstract, characters 219–350 · show in text ↑
e6

“As a result of the ensilage process, decreases in crude fiber and hemicellulose were observed in the HS and HF silages”

✓ located verbatim in the source · abstract, characters 1032–1150 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

Hemp ensiling with biological additives is an effective pre-treatment that can preserve hemp biomass for biofuel production.

Treatment
ensiling with biological additivese7
Outcome
preserve the biomasse7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Cannabis sativa subsp. sativa
Cannabis sativa subsp. sativa · TaxonNo machine-readable author provided. Bastique assumed (based on copyright claims). · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e7

“hemp ensiling with biological additives is an effective pre-treatment of hemp plants for subsequent biofuel production that can preserve the biomass”

✓ located verbatim in the source · abstract, characters 1158–1306 · show in text ↑

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inoculation · molecular: metabolites · Cannabis sativaResults agree

5 papers: 5 positive, 0 negative or no effect

4 papers in the same direction as this paper

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  2. positive
  3. positive

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.