Abstract
Chilling stress seriously limits grain yield and quality worldwide. However, the genes and the underlying mechanisms that respond to chilling stress remain elusive. This study identified ABF1, a cold-induced transcription factor of the bZIP family. Disruption of ABF1 impaired chilling tolerance with increased ion leakage and reduced proline contents, while ABF1 over-expression lines exhibited the opposite tendency, suggesting that ABF1 positively regulated chilling tolerance in rice. Moreover, SnRK2 protein kinase SAPK10 could phosphorylate ABF1, and strengthen the DNA-binding ability of ABF1 to the G-box cis-element of the promoter of TPS2, a positive regulator of trehalose biosynthesis, consequently elevating the TPS2 transcription and the endogenous trehalose contents. Meanwhile, applying exogenous trehalose enhanced the chilling tolerance of abf1 mutant lines. In summary, this study provides a novel pathway ‘SAPK10-ABF1-TPS2’ involved in rice chilling tolerance through regulating trehalose homeostasis.
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Claims 10
Claim · model-written summary
ABF1 was identified as a cold-induced bZIP transcription factor.
- Taxon
- Rice
Oryza sativat0 - Gene
- ABF1e0
- Outcome
- ABF1, a cold-induced transcription factore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This study identified ABF1, a cold-induced transcription factor of the bZIP family”
t0“Rice”
Claim · model-written summary
Disruption of ABF1 impaired chilling tolerance.
- Taxon
- RiceOryza sativat0
- Treatment
- Disruption of ABF1e0
- Gene
- ABF1e0
- Outcome
- chilling tolerancee0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of ABF1 impaired chilling tolerance”
t0“Rice”
Claim · model-written summary
Disruption of ABF1 increased ion leakage.
- Taxon
- RiceOryza sativat0
- Treatment
- Disruption of ABF1e0
- Gene
- ABF1e0
- Outcome
- ion leakagee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of ABF1 impaired chilling tolerance with increased ion leakage”
t0“Rice”
Claim · model-written summary
Disruption of ABF1 reduced proline content.
- Taxon
- RiceOryza sativat0
- Treatment
- Disruption of ABF1e0
- Gene
- ABF1e0
- Outcome
- proline contentse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of ABF1 impaired chilling tolerance with increased ion leakage and reduced proline contents”
t0“Rice”
Claim · model-written summary
ABF1 overexpression showed the opposite tendency for chilling tolerance, ion leakage and proline content.
- Taxon
- RiceOryza sativat0
- Treatment
- ABF1 over-expression linese0
- Gene
- ABF1e0
- Outcome
- opposite tendencye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABF1 over-expression lines exhibited the opposite tendency”
t0“Rice”
Claim · model-written summary
SAPK10 phosphorylated ABF1.
- Taxon
- RiceOryza sativat0
- Gene
- SnRK2 protein kinase SAPK10e0
- Outcome
- phosphorylate ABF1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“SnRK2 protein kinase SAPK10 could phosphorylate ABF1”
t0“Rice”
Claim · model-written summary
SAPK10 strengthened ABF1 binding to the TPS2 promoter G-box.
- Taxon
- RiceOryza sativat0
- Gene
- TPS2e0
- Outcome
- DNA-binding ability of ABF1e0
- Qualifier
- G-box cis-elemente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“strengthen the DNA-binding ability of ABF1 to the G-box cis-element of the promoter of TPS2”
t0“Rice”
Claim · model-written summary
The pathway elevated TPS2 transcription.
- Taxon
- RiceOryza sativat0
- Gene
- TPS2e0
- Outcome
- TPS2 transcriptione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“consequently elevating the TPS2 transcription”
t0“Rice”
Claim · model-written summary
The pathway elevated endogenous trehalose content.
- Taxon
- RiceOryza sativat0
- Outcome
- endogenous trehalose contentse0
- Qualifier
- endogenous trehalose contentse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“and the endogenous trehalose contents”
t0“Rice”
Claim · model-written summary
Exogenous trehalose enhanced chilling tolerance of abf1 mutant lines.
- Taxon
- RiceOryza sativat0
- Material
- abf1 mutant linese0
- Treatment
- applying exogenous trehalosee0
- Outcome
- chilling tolerancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“applying exogenous trehalose enhanced the chilling tolerance of abf1 mutant lines”
t0“Rice”
The same questions in other papers
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See all 31 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.