Abstract
The wheat NAC transcription factor TaNACL-D1 enhances resistance to the economically devastating Fusarium head blight (FHB) disease. The objective of this study was to decipher the alterations in gene expression, pathways and biological processes that led to enhanced resistance as a result of the constitutive expression of TaNACL-D1 in wheat. Transcriptomic analysis was used to determine the genes and processes enhanced in wheat due to TaNACL-D1 overexpression, both in the presence and absence of the causal agent of FHB, Fusarium graminearum (0- and 1-day post-treatment). The overexpression of TaNACL-D1 resulted in more pronounced transcriptional reprogramming as a response to fungal infection, leading to the enhanced expression of genes involved in detoxification, immune responses, secondary metabolism, hormone biosynthesis, and signalling. The regulation and response to JA and ABA were differentially regulated between the OE and the WT. Furthermore, the results suggest that the OE may more efficiently: (i) regulate the oxidative burst; (ii) modulate cell death; and (iii) induce both the phenylpropanoid pathway and lignin synthesis. Thus, this study provides insights into the mode of action and downstream target pathways for this novel NAC transcription factor, further validating its potential as a gene to enhance FHB resistance in wheat.
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Claims 6
Claim · model-written summary
TaNACL-D1 overexpression enhanced resistance to Fusarium head blight.
- Taxon
- wheat
Triticum aestivume0 - Treatment
- TaNACL-D1e0
- Gene
- TaNACL-D1e0
- Outcome
- resistance to the economically devastating Fusarium head blight (FHB) diseasee0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The wheat NAC transcription factor TaNACL-D1 enhances resistance to the economically devastating Fusarium head blight (FHB) disease.”
Claim · model-written summary
Overexpression produced more pronounced infection-responsive transcriptional reprogramming and enhanced expression of defence-related genes.
- Taxon
- wheatTriticum aestivume1
- Treatment
- overexpression of TaNACL-D1e0
- Interactor
- Fusarium graminearum
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=60)
Fusarium graminearume1pathogen - Gene
- TaNACL-D1e0
- Outcome
- transcriptional reprogramminge0
- Direction
- more pronouncede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=500)
Verbatim
e0“The overexpression of TaNACL-D1 resulted in more pronounced transcriptional reprogramming as a response to fungal infection, leading to the enhanced expression of genes involved in detoxification, immune responses, secondary metabolism, hormone biosynthesis, and signalling.”
e1“in wheat due to TaNACL-D1 overexpression, both in the presence and absence of the causal agent of FHB, Fusarium graminearum”
Claim · model-written summary
JA and ABA regulation and response differed between overexpression and wild type.
- Taxon
- wheatTriticum aestivume1
- Material
- the OEe0
- Outcome
- regulation and response to JA and ABAe0
- Comparator
- the WTe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The regulation and response to JA and ABA were differentially regulated between the OE and the WT.”
e1“in wheat due to TaNACL-D1 overexpression”
Claim · model-written summary
The overexpressor may regulate oxidative burst more efficiently.
- Taxon
- wheatTriticum aestivume1
- Material
- the OEe0
- Outcome
- regulate the oxidative burste0
- Qualifier
- may more efficientlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the OE may more efficiently: (i) regulate the oxidative burst; (ii) modulate cell death; and (iii) induce both the phenylpropanoid pathway and lignin synthesis.”
e1“in wheat due to TaNACL-D1 overexpression”
Claim · model-written summary
The overexpressor may modulate cell death more efficiently.
- Taxon
- wheatTriticum aestivume1
- Material
- the OEe0
- Outcome
- modulate cell deathe0
- Qualifier
- may more efficientlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the OE may more efficiently: (i) regulate the oxidative burst; (ii) modulate cell death; and (iii) induce both the phenylpropanoid pathway and lignin synthesis.”
e1“in wheat due to TaNACL-D1 overexpression”
Claim · model-written summary
The overexpressor may induce the phenylpropanoid pathway and lignin synthesis more efficiently.
- Taxon
- wheatTriticum aestivume1
- Material
- the OEe0
- Outcome
- induce both the phenylpropanoid pathway and lignin synthesise0
- Qualifier
- may more efficientlye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the OE may more efficiently: (i) regulate the oxidative burst; (ii) modulate cell death; and (iii) induce both the phenylpropanoid pathway and lignin synthesis.”
e1“in wheat due to TaNACL-D1 overexpression”