Abstract
Manganese (Mn) is among one of the essential trace elements for normal plant development; however, excessive Mn can cause plant growth and development to be hindered. Nevertheless, the regulatory mechanisms of plant root response to Mn poisoning remain unclear. In the present study, results revealed that the root growth was inhibited when exposed to Mn poisoning. Physiological results showed that the antioxidase enzyme activities (peroxidase, superoxide dismutase, ascorbate peroxidase, and catalase) and the proline, malondialdehyde, and soluble sugar contents increased significantly under Mn toxicity stress (100 μM Mn), whereas the soluble protein and four hormones' (indolebutyric acid, abscisic acid, indoleacetic acid, and gibberellic acid 3) contents decreased significantly. In addition, the Mn, Fe, Na, Al, and Se contents in the roots increased significantly, whereas those of Mg, Zn, and K decreased significantly. Furthermore, RNA sequencing (RNA-seq) analysis was used to test the differentially expressed genes (DEGs) of soybean root under Mn poisoning. The results found 45,274 genes in soybean root and 1430 DEGs under Mn concentrations of 5 (normal) and 100 (toxicity) μM. Among these DEGs, 572 were upregulated and 858 were downregulated, indicating that soybean roots may initiate complex molecular regulatory mechanisms on Mn poisoning stress. The results of quantitative RT-PCR indicated that many DEGs were upregulated or downregulated markedly in the roots, suggesting that the regulation of DEGs may be complex. Therefore, the regulatory mechanism of soybean root on Mn toxicity stress is complicated. Present results lay the foundation for further study on the molecular regulation mechanism of function genes involved in regulating Mn tolerance traits in soybean roots.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 11
Claim · model-written summary
Manganese poisoning inhibited root growth.
- Taxon
- Soybean
Glycine maxt0 - Treatment
- Mn poisoninge0
- Outcome
- root growthe0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean”
e0“root growth was inhibited when exposed to Mn poisoning.”
Claim · model-written summary
Antioxidase enzyme activities rose significantly under 100 μM Mn.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- Mn toxicity stress (100 μM Mn)e0
- Outcome
- antioxidase enzyme activities (peroxidase, superoxide dismutase, ascorbate peroxidase, and catalase)e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean”
e0“antioxidase enzyme activities (peroxidase, superoxide dismutase, ascorbate peroxidase, and catalase) and the proline, malondialdehyde, and soluble sugar contents increased significantly under Mn toxicity stress (100 μM Mn)”
Claim · model-written summary
Proline, malondialdehyde and soluble sugar increased significantly.
- Taxon
- SoybeanGlycine maxt0
- Treatment
- Mn toxicity stress (100 μM Mn)e0
- Outcome
- proline, malondialdehyde, and soluble sugar contentse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean”
e0“proline, malondialdehyde, and soluble sugar contents increased significantly under Mn toxicity stress (100 μM Mn)”
Claim · model-written summary
Soluble protein and four hormone contents decreased significantly.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- soluble protein and four hormones' (indolebutyric acid, abscisic acid, indoleacetic acid, and gibberellic acid 3) contentse0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean”
e0“soluble protein and four hormones' (indolebutyric acid, abscisic acid, indoleacetic acid, and gibberellic acid 3) contents decreased significantly.”
Claim · model-written summary
Root Mn, Fe, Na, Al and Se content increased significantly.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- Mn, Fe, Na, Al, and Se contents in the rootse0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean”
e0“Mn, Fe, Na, Al, and Se contents in the roots increased significantly”
Claim · model-written summary
Root Mg, Zn and K contents decreased significantly.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- Mg, Zn, and Ke0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean”
e0“those of Mg, Zn, and K decreased significantly”
Claim · model-written summary
Soybean roots had 45,274 detected genes.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- genes in soybean roote0
- Value
- 45,274e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean”
e0“The results found 45,274 genes in soybean root”
Claim · model-written summary
There were 1430 DEGs between 5 and 100 μM Mn.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean”
e0“1430 DEGs under Mn concentrations of 5 (normal) and 100 (toxicity) μM”
Claim · model-written summary
Of the DEGs, 572 were upregulated.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- DEGse0
- Direction
- upregulatede0
- Value
- 572e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean”
e0“Among these DEGs, 572 were upregulated”
Claim · model-written summary
Of the DEGs, 858 were downregulated.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- downregulatede0
- Value
- 858e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean”
e0“858 were downregulated”
Claim · model-written summary
RT-PCR indicated many DEGs were markedly upregulated or downregulated.
- Taxon
- SoybeanGlycine maxt0
- Outcome
- DEGse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean”
e0“many DEGs were upregulated or downregulated markedly in the roots”
The same questions in other papers
manganese atom · growth · Glycine max (soybean)Results differ
17 papers in the same direction as this paper · 13 in the opposite direction
- negative
- positive
- positive
27 papers more in this group
See all 31 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.