Abstract
In order to investigate the effects of Manganese (Mn) toxicity stress on the growth and gene expression at the seedling stage of soybean, soybean seedlings were treated with normal Mn concentration (5 μmol/L MnSO4) and excess Mn concentration (100 μmol/L MnSO4) by the method of hydroponic culture in this study. When soybean was subjected to Mn toxicity stress, excessive Mn could affect seedling growth, root development, the number of Mn oxide spots in leaves, and the Mn accumulation content in different parts of soybean. With the increase of exogenous Mn concentration and the prolongation of culture time, the shoot and root biomasses of soybean decreased significantly, but the plant height of soybean had no obvious effects. The total root length, root surface area and root volume of soybean decreased significantly, but the taproot length, taproot tip length and root diameter did not change significantly. The number of Mn spots in the primary leaves (first leaf) was significantly more than that in the old leaves (second leaf) and the youngest leaves (fourth leaf). The Mn concentration in leaves was significantly higher than that in roots, and the Mn concentration in the old leaves was significantly higher than that in youngest leaves with the method of inductively coupled plasma atomic emission spectrometry (ICP-AES). Moreover, the results in the present study suggested that the 10 selected genes were significantly up-regulated or down-regulated by Mn toxicity in the old and young leaves by quantitative real-time PCR (qRT-PCR) analysis. This indicates that these genes might be important in the process of regulation in old and young leaves of the physiological responses and ion transporting to Mn toxicity stress.
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Claims 9
Claim · model-written summary
Excess Mn decreased the shoot and root biomass of soybean significantly.
- Taxon
- soybean
Glycine maxe0 - Treatment
- excess Mn concentration (100 μmol/L MnSO4)e0
- Outcome
- shoot and root biomassese1
- Direction
- decreasede1
- Comparator
- normal Mn concentration (5 μmol/L MnSO4)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybean seedlings were treated with normal Mn concentration (5 μmol/L MnSO4) and excess Mn concentration (100 μmol/L MnSO4)”
e1“the shoot and root biomasses of soybean decreased significantly”
Claim · model-written summary
Excess Mn decreased total root length, root surface area and root volume of soybean significantly.
- Taxon
- soybeanGlycine maxe0
- Treatment
- excess Mn concentration (100 μmol/L MnSO4)e0
- Outcome
- total root length, root surface area and root volumee2
- Direction
- decreasede2
- Comparator
- normal Mn concentration (5 μmol/L MnSO4)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybean seedlings were treated with normal Mn concentration (5 μmol/L MnSO4) and excess Mn concentration (100 μmol/L MnSO4)”
e2“The total root length, root surface area and root volume of soybean decreased significantly”
Claim · model-written summary
Excess Mn had no obvious effect on soybean plant height.
- Taxon
- soybeanGlycine maxe0
- Treatment
- excess Mn concentration (100 μmol/L MnSO4)e0
- Outcome
- plant heighte3
- Direction
- no obvious effectse3
- Comparator
- normal Mn concentration (5 μmol/L MnSO4)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybean seedlings were treated with normal Mn concentration (5 μmol/L MnSO4) and excess Mn concentration (100 μmol/L MnSO4)”
e3“the plant height of soybean had no obvious effects”
Claim · model-written summary
Excess Mn did not significantly change taproot length, taproot tip length or root diameter of soybean.
- Taxon
- soybeanGlycine maxe0
- Treatment
- excess Mn concentration (100 μmol/L MnSO4)e0
- Outcome
- taproot length, taproot tip length and root diametere4
- Direction
- did not change significantlye4
- Comparator
- normal Mn concentration (5 μmol/L MnSO4)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybean seedlings were treated with normal Mn concentration (5 μmol/L MnSO4) and excess Mn concentration (100 μmol/L MnSO4)”
e4“the taproot length, taproot tip length and root diameter did not change significantly”
Claim · model-written summary
Mn spots were significantly more numerous on the primary leaves (first leaf) than on the old (second) and youngest (fourth) leaves.
- Taxon
- soybeanGlycine maxe0
- Organ / stage
- primary leaves (first leaf)e5
- Outcome
- number of Mn spotse5
- Direction
- significantly moree5
- Comparator
- old leaves (second leaf)e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybean seedlings were treated with normal Mn concentration (5 μmol/L MnSO4) and excess Mn concentration (100 μmol/L MnSO4)”
e5“The number of Mn spots in the primary leaves (first leaf) was significantly more than that in the old leaves (second leaf) and the youngest leaves (fourth leaf)”
Claim · model-written summary
Mn concentration in soybean leaves was significantly higher than in roots.
- Taxon
- soybeanGlycine maxe0
- Organ / stage
- leavese6
- Compound
- Mne6
- Outcome
- Mn concentratione6
- Direction
- significantly highere6
- Comparator
- rootse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybean seedlings were treated with normal Mn concentration (5 μmol/L MnSO4) and excess Mn concentration (100 μmol/L MnSO4)”
e6“The Mn concentration in leaves was significantly higher than that in roots”
Claim · model-written summary
Mn concentration in the old leaves of soybean was significantly higher than in the youngest leaves.
- Taxon
- soybeanGlycine maxe0
- Organ / stage
- old leavese7
- Compound
- Mne7
- Outcome
- Mn concentratione7
- Direction
- significantly highere7
- Comparator
- youngest leavese7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybean seedlings were treated with normal Mn concentration (5 μmol/L MnSO4) and excess Mn concentration (100 μmol/L MnSO4)”
e7“the Mn concentration in the old leaves was significantly higher than that in youngest leaves”
Claim · model-written summary
Mn toxicity significantly up- or down-regulated ten selected genes in old and young soybean leaves.
- Taxon
- soybeanGlycine maxe0
- Organ / stage
- old and young leavese6
- Treatment
- Mn toxicitye6
- Gene
- 10 selected genese6
- Outcome
- significantly up-regulated or down-regulatede6
- Direction
- up-regulated or down-regulatede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybean seedlings were treated with normal Mn concentration (5 μmol/L MnSO4) and excess Mn concentration (100 μmol/L MnSO4)”
e6“the 10 selected genes were significantly up-regulated or down-regulated by Mn toxicity in the old and young leaves”
Claim · model-written summary
Ten selected genes may be important in regulating physiological responses and ion transport to Mn toxicity in soybean leaves.
- Taxon
- soybeanGlycine maxe0
- Gene
- these genese6
- Outcome
- physiological responses and ion transporting to Mn toxicity stresse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“soybean seedlings were treated with normal Mn concentration (5 μmol/L MnSO4) and excess Mn concentration (100 μmol/L MnSO4)”
e6“these genes might be important in the process of regulation in old and young leaves of the physiological responses and ion transporting to Mn toxicity stress”
The same questions in other papers
manganese atom · growth · Glycine max (soybean)Results differ
17 papers in the same direction as this paper · 13 in the opposite direction
- negative
- positive
- positive
27 papers more in this group
See all 31 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.