Abstract
Fusarium graminearum, the primary pathogenic fungus responsible for Fusarium head blight (FHB) in wheat, secretes abundant chemical compounds that interact with host plants. In this study, a secreted protein FgHrip1, isolated from the culture filtrate of F. graminearum, was found to induce typical cell death in tobacco. The FgHrip1 gene was then cloned and expressed in Escherichia coli. Further bioassay analysis showed that the recombinant FgHrip1 induced early defense induction events, such as reactive oxygen species (ROS) production, callose deposition, and up-regulation of defense-related genes in tobacco. Furthermore, FgHrip1 significantly enhanced immunity in tobacco seedlings against Pseudomonas syringae pv. tabaci 6605 (Pst. 6605) and tobacco mosaic virus (TMV). FgHrip1-treated wheat spikes also exhibited defense-related transcript accumulation and developed immunity against FHB infection. Whereas the expression of FgHrip1 was induced during the infection process, the deletion of the gene impaired the virulence of F. graminearum. Our results suggest that FgHrip1 triggers immunity and induces disease resistance in tobacco and wheat, thereby providing new insight into strategy for biocontrol of FHB.
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Claims 8
Claim · model-written summary
FgHrip1 induced cell death in tobacco.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- FgHrip1e0
- Interactor
- F. graminearum
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=60)
Fusarium graminearume0pathogen - Gene
- FgHrip1e0
- Outcome
- cell deathe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=500)
Verbatim
e0“FgHrip1, isolated from the culture filtrate of F. graminearum, was found to induce typical cell death in tobacco”
Claim · model-written summary
Recombinant FgHrip1 induced ROS production, callose deposition, and defense gene upregulation in tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- recombinant FgHrip1e0
- Gene
- FgHrip1e0
- Outcome
- reactive oxygen species (ROS) production, callose deposition, and up-regulation of defense-related genese0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“recombinant FgHrip1 induced early defense induction events, such as reactive oxygen species (ROS) production, callose deposition, and up-regulation of defense-related genes in tobacco”
Claim · model-written summary
FgHrip1 enhanced tobacco immunity to Pseudomonas syringae pv. tabaci 6605.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- FgHrip1e0
- Interactor
- Pseudomonas syringae pv. tabaci 6605Pseudomonas amygdali pv. tabaci str. 6605e0pathogen
- Outcome
- immunitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FgHrip1 significantly enhanced immunity in tobacco seedlings against Pseudomonas syringae pv. tabaci 6605 (Pst. 6605) and tobacco mosaic virus (TMV)”
Claim · model-written summary
FgHrip1 enhanced tobacco immunity to tobacco mosaic virus.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- FgHrip1e0
- Interactor
- tobacco mosaic virus
Tobacco mosaic viruse0pathogen - Outcome
- immunitye0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FgHrip1 significantly enhanced immunity in tobacco seedlings against Pseudomonas syringae pv. tabaci 6605 (Pst. 6605) and tobacco mosaic virus (TMV)”
Claim · model-written summary
FgHrip1 treatment increased defense transcript accumulation in wheat spikes.
- Taxon
- wheat
Triticum aestivume0 - Treatment
- FgHrip1-treatede0
- Outcome
- defense-related transcript accumulatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FgHrip1-treated wheat spikes also exhibited defense-related transcript accumulation”
Claim · model-written summary
FgHrip1-treated wheat spikes developed immunity against FHB.
- Taxon
- wheatTriticum aestivume0
- Treatment
- FgHrip1-treatede0
- Interactor
- FHBe0pathogen
- Outcome
- immunity against FHB infectione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FgHrip1-treated wheat spikes also exhibited defense-related transcript accumulation and developed immunity against FHB infection”
Claim · model-written summary
FgHrip1 expression was induced during infection.
- Taxon
- Fusarium graminearumFusarium graminearumt0
- Gene
- FgHrip1e0
- Outcome
- expression of FgHrip1e0
- Direction
- inducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“expression of FgHrip1 was induced during the infection process”
t0“Fusarium graminearum”
Claim · model-written summary
Deleting FgHrip1 impaired F. graminearum virulence.
- Taxon
- Fusarium graminearumFusarium graminearumt0
- Treatment
- deletion of the genee0
- Gene
- FgHrip1t0
- Outcome
- virulence of F. graminearume0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“deletion of the gene impaired the virulence of F. graminearum”
t0“FgHrip1 from Fusarium graminearum”
The same questions in other papers
loss of function · disease · Fusarium graminearumPositive and negative results
2 papers in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.