Abstract
The Kin1/Par-1/MARK kinases regulate various cellular processes in eukaryotic organisms. Kin1 orthologs are well conserved in fungal pathogens but none of them have been functionally characterized. Here, we show that KIN1 is important for pathogenesis and growth in two phytopathogenic fungi and that FgKin1 regulates ascospore germination and the localization of Tub1 β-tubulins in Fusarium graminearum. The Fgkin1 mutant and putative FgKIN1(S172A) kinase dead (nonactivatable) transformants were characterized for defects in plant infection, sexual and asexual reproduction, and stress responses. The localization of FgKin1 and two β-tubulins were examined in the wild-type and mutant backgrounds. Deletion of FgKIN1 resulted in reduced virulence and defects in ascospore germination and release. FgKin1 localized to the center of septal pores. FgKIN1 deletion had no effect on Tub2 microtubules but disrupted Tub1 localization. In the mutant, Tub1 appeared to be enriched in the nucleolus. In Magnaporthe oryzae, MoKin1 has similar functions in growth and infection and it also localizes to septal pores. The S172A mutation had no effect on the localization and function of FgKIN1 during sexual reproduction. These results indicate that FgKIN1 has kinase-dependent and independent functions and it specifically regulates Tub1 β-tubulins. FgKin1 plays a critical role in ascospore discharge, germination, and plant infection.
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Claims 10
Claim · model-written summary
Deleting FgKIN1 reduced virulence of Fusarium graminearum.
- Taxon
- Fusarium graminearum
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=60)
Fusarium graminearumt0 - Treatment
- Deletion of FgKIN1e1
- Gene
- FgKIN1e1
- Outcome
- virulencee1
- Direction
- reducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=500)
Verbatim
e1“Deletion of FgKIN1 resulted in reduced virulence and defects in ascospore germination and release”
t0“Fusarium graminearum”
Claim · model-written summary
Deleting FgKIN1 caused defects in ascospore germination and release.
- Taxon
- Fusarium graminearumFusarium graminearumt0
- Treatment
- Deletion of FgKIN1e1
- Outcome
- ascospore germination and releasee1
- Qualifier
- defectse1
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Deletion of FgKIN1 resulted in reduced virulence and defects in ascospore germination and release”
t0“Fusarium graminearum”
Claim · model-written summary
FgKIN1 deletion had no effect on Tub2 microtubules.
- Taxon
- Fusarium graminearumFusarium graminearumt0
- Treatment
- FgKIN1 deletione2
- Outcome
- Tub2 microtubulese2
- Direction
- no effecte2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“FgKIN1 deletion had no effect on Tub2 microtubules but disrupted Tub1 localization”
t0“Fusarium graminearum”
Claim · model-written summary
FgKIN1 deletion disrupted Tub1 localization.
- Taxon
- Fusarium graminearumFusarium graminearumt0
- Treatment
- FgKIN1 deletione2
- Outcome
- Tub1 localizatione2
- Direction
- disruptede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“FgKIN1 deletion had no effect on Tub2 microtubules but disrupted Tub1 localization”
t0“Fusarium graminearum”
Claim · model-written summary
In the mutant, Tub1 was enriched in the nucleolus relative to wild-type.
- Taxon
- Fusarium graminearumFusarium graminearumt0
- Treatment
- mutante3
- Outcome
- Tub1e3
- Direction
- enrichede3
- Qualifier
- in the nucleoluse3
- Comparator
- wild-typee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“In the mutant, Tub1 appeared to be enriched in the nucleolus”
e4“The localization of FgKin1 and two β-tubulins were examined in the wild-type and mutant backgrounds”
t0“Fusarium graminearum”
Claim · model-written summary
The S172A kinase-dead mutation had no effect on FgKIN1 localization and function during sexual reproduction.
- Taxon
- Fusarium graminearumFusarium graminearumt0
- Treatment
- S172A mutatione5
- Gene
- FgKIN1e5
- Outcome
- localization and function of FgKIN1 during sexual reproductione5
- Direction
- no effecte5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“The S172A mutation had no effect on the localization and function of FgKIN1 during sexual reproduction”
t0“Fusarium graminearum”
Claim · model-written summary
FgKin1 localizes to the center of septal pores in Fusarium graminearum.
- Taxon
- Fusarium graminearumFusarium graminearumt0
- Gene
- FgKin1e6
- Outcome
- localizede6
- Qualifier
- center of septal porese6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“FgKin1 localized to the center of septal pores”
t0“Fusarium graminearum”
Claim · model-written summary
MoKin1 has functions in growth and infection similar to FgKin1 in Magnaporthe oryzae.
- Taxon
- Magnaporthe oryzae
Pyricularia oryzaee7 - Gene
- MoKin1e7
- Outcome
- growth and infectione7
- Qualifier
- similar functionse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“In Magnaporthe oryzae, MoKin1 has similar functions in growth and infection and it also localizes to septal pores”
Claim · model-written summary
MoKin1 localizes to septal pores in Magnaporthe oryzae.
- Taxon
- Magnaporthe oryzaePyricularia oryzaee7
- Gene
- MoKin1e7
- Outcome
- localizese7
- Qualifier
- to septal porese7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“In Magnaporthe oryzae, MoKin1 has similar functions in growth and infection and it also localizes to septal pores”
Claim · model-written summary
FgKIN1 has kinase-dependent and kinase-independent functions and specifically regulates Tub1 β-tubulins.
- Taxon
- Fusarium graminearumFusarium graminearumt0
- Gene
- FgKIN1e1
- Outcome
- kinase-dependent and independent functionse1
- Qualifier
- specifically regulates Tub1 β-tubulinse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“These results indicate that FgKIN1 has kinase-dependent and independent functions and it specifically regulates Tub1 β-tubulins”
t0“Fusarium graminearum”
The same questions in other papers
loss of function · pest · Fusarium graminearumPositive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
loss of function · disease · Fusarium graminearumPositive and negative results
1 paper in the same direction as this paper · 3 in the opposite direction
- positive
- negative
- negative
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.