Abstract
Osmotic stress caused by low-temperature, drought and salinity was a prevalent abiotic stress in plant that severely inhibited plant development and agricultural yield, particularly in tea plant. Jasmonic acid (JA) is an important phytohormone involving in plant stress. However, underlying molecular mechanisms of JA modulated osmotic stress response remains unclear. In this study, high concentration of mannitol induced JA accumulation and increase of peroxidase activity in tea plant. Integrated transcriptome mined a JA signaling master, MYC2 transcription factor is shown as a hub regulator that induced by mannitol, expression of which positively correlated with JA biosynthetic genes (LOX and AOS) and peroxidase genes (PER). CsMYC2 was determined as a nuclei-localized transcription activator, furthermore, Protein-DNA interaction analysis indicated that CsMYC2 was positive regulator that activated the transcription of CsLOX7, CsAOS2, CsPER1 and CsPER3 via bound with their promoters, respectively. Suppression of CsMYC2 expression resulted in a reduced JA content and peroxidase activity and osmotic stress tolerance of tea plant. Overexpression of CsMYC2 in Arabidopsis improved JA content, peroxidase activity and plants tolerance against mannitol stress. Together, we proposed a positive feedback loop mediated by CsMYC2, CsLOX7 and CsAOS2 which constituted to increase the tolerance of osmotic stress through fine-tuning the accumulation of JA levels and increase of POD activity in tea plant.
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Claims 17
Claim · model-written summary
Mannitol induced JA accumulation in tea plants.
Not reportedComparator
Verbatim
e0“high concentration of mannitol induced JA accumulation”
t0“tea plant”
Claim · model-written summary
Mannitol increased peroxidase activity in tea plants.
- Taxon
- tea plantt0
- Treatment
- high concentration of mannitole0
- Outcome
- peroxidase activitye0
- Direction
- increasee0
Not reportedComparator
Verbatim
e0“high concentration of mannitol induced JA accumulation and increase of peroxidase activity in tea plant”
t0“tea plant”
Claim · model-written summary
Mannitol induced MYC2 expression.
Not reportedComparator
Verbatim
e0“MYC2 transcription factor is shown as a hub regulator that induced by mannitol”
t0“tea plant”
Claim · model-written summary
MYC2 expression positively correlated with LOX and AOS expression.
Verbatim
e0“MYC2 transcription factor is shown as a hub regulator that induced by mannitol, expression of which positively correlated with JA biosynthetic genes (LOX and AOS)”
t0“tea plant”
Claim · model-written summary
MYC2 expression positively correlated with PER expression.
Verbatim
e0“MYC2 transcription factor is shown as a hub regulator that induced by mannitol, expression of which positively correlated with JA biosynthetic genes (LOX and AOS) and peroxidase genes (PER)”
t0“tea plant”
Claim · model-written summary
CsMYC2 was a nuclear localized transcription activator.
Verbatim
e0“CsMYC2 was determined as a nuclei-localized transcription activator”
t0“tea plant”
Claim · model-written summary
CsMYC2 activated transcription of CsLOX7 by binding its promoter.
Verbatim
e0“CsMYC2 was positive regulator that activated the transcription of CsLOX7, CsAOS2, CsPER1 and CsPER3 via bound with their promoters”
t0“tea plant”
Claim · model-written summary
CsMYC2 activated transcription of CsAOS2 by binding its promoter.
Verbatim
e0“CsMYC2 was positive regulator that activated the transcription of CsLOX7, CsAOS2, CsPER1 and CsPER3 via bound with their promoters”
t0“tea plant”
Claim · model-written summary
CsMYC2 activated transcription of CsPER1 by binding its promoter.
Verbatim
e0“CsMYC2 was positive regulator that activated the transcription of CsLOX7, CsAOS2, CsPER1 and CsPER3 via bound with their promoters”
t0“tea plant”
Claim · model-written summary
CsMYC2 activated transcription of CsPER3 by binding its promoter.
Verbatim
e0“CsMYC2 was positive regulator that activated the transcription of CsLOX7, CsAOS2, CsPER1 and CsPER3 via bound with their promoters”
t0“tea plant”
Claim · model-written summary
Suppressing CsMYC2 reduced tea plant JA content.
- Taxon
- tea plantt0
- Treatment
- Suppression of CsMYC2 expressione0
- Gene
- CsMYC2e0
- Outcome
- JA contente0
- Direction
- reducede0
Not reportedComparator
Verbatim
e0“Suppression of CsMYC2 expression resulted in a reduced JA content”
t0“tea plant”
Claim · model-written summary
Suppressing CsMYC2 reduced tea plant peroxidase activity.
- Taxon
- tea plantt0
- Treatment
- Suppression of CsMYC2 expressione0
- Gene
- CsMYC2e0
- Outcome
- peroxidase activitye0
- Direction
- reducede0
Not reportedComparator
Verbatim
e0“Suppression of CsMYC2 expression resulted in a reduced JA content and peroxidase activity”
t0“tea plant”
Claim · model-written summary
Suppressing CsMYC2 reduced tea plant osmotic stress tolerance.
- Taxon
- tea plantt0
- Treatment
- Suppression of CsMYC2 expressione0
- Gene
- CsMYC2e0
- Outcome
- osmotic stress tolerancee0
- Direction
- reducede0
Not reportedComparator
Verbatim
e0“Suppression of CsMYC2 expression resulted in a reduced JA content and peroxidase activity and osmotic stress tolerance of tea plant”
t0“tea plant”
Claim · model-written summary
Overexpressing CsMYC2 increased JA content in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- Overexpression of CsMYC2e0
- Gene
- CsMYC2e0
- Outcome
- JA contente0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of CsMYC2 in Arabidopsis improved JA content”
Claim · model-written summary
Overexpressing CsMYC2 increased peroxidase activity in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Overexpression of CsMYC2e0
- Gene
- CsMYC2e0
- Outcome
- peroxidase activitye0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of CsMYC2 in Arabidopsis improved JA content, peroxidase activity”
Claim · model-written summary
Overexpressing CsMYC2 improved Arabidopsis tolerance to mannitol.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Overexpression of CsMYC2e0
- Gene
- CsMYC2e0
- Outcome
- plants tolerance against mannitol stresse0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of CsMYC2 in Arabidopsis improved JA content, peroxidase activity and plants tolerance against mannitol stress”
Claim · model-written summary
Authors propose a CsMYC2 CsLOX7 CsAOS2 feedback loop increasing tea plant osmotic stress tolerance through JA accumulation and POD activity.
Verbatim
e0“a positive feedback loop mediated by CsMYC2, CsLOX7 and CsAOS2 which constituted to increase the tolerance of osmotic stress through fine-tuning the accumulation of JA levels and increase of POD activity in tea plant”
t0“tea plant”
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6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.