Abstract
Rice sheath blight (ShB) is a devastating disease that severely threatens rice production worldwide. Induction of cell death represents a key step during infection by the ShB pathogen Rhizoctonia solani. Nonetheless, the underlying mechanisms remain largely unclear. In the present study, we identified a rice transcription factor, OsERF65, that negatively regulates resistance to ShB by suppressing cell death. OsERF65 was significantly upregulated by R. solani infection in susceptible cultivar Lemont and was highly expressed in the leaf sheath. Overexpression of OsERF65 (OsERF65OE) decreased rice resistance, while the knockout mutant (oserf65) exhibited significantly increased resistance against ShB. The transcriptome assay revealed that OsERF65 repressed the expression of peroxidase genes after R. solani infection. The antioxidative enzyme activity was significantly increased in oserf65 plants but reduced in OsERF65OE plants. Consistently, hydrogen peroxide content was apparently reduced in oserf65 plants but accumulated in OsERF65OE plants. OsERF65 directly bound to the GCC box in the promoter regions of four peroxidase genes and suppressed their transcription, reducing the ability to scavenge reactive oxygen species (ROS). The oserf65 mutant exhibited a slight decrease in plant height but increased grain yield. Overall, our results revealed an undocumented role of OsERF65 that acts as a crucial regulator of rice resistance to R. solani and a potential target for improving both ShB resistance and rice yield.
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Claims 13
Claim · model-written summary
R. solani infection significantly upregulated OsERF65 in susceptible rice cultivar Lemont.
- Taxon
- rice
Oryza sativae1 - Material
- Lemonte2
- Treatment
- R. solani infectione2
- Gene
- OsERF65e2
- Outcome
- OsERF65e2
- Direction
- significantly upregulatede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Rice sheath blight (ShB) is a devastating disease”
e2“OsERF65 was significantly upregulated by R. solani infection in susceptible cultivar Lemont and was highly expressed in the leaf sheath”
Claim · model-written summary
OsERF65 was highly expressed in the rice leaf sheath.
- Taxon
- riceOryza sativae1
- Organ / stage
- leaf sheathe2
- Gene
- OsERF65e2
- Outcome
- highly expressed in the leaf sheathe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Rice sheath blight (ShB) is a devastating disease”
e2“OsERF65 was significantly upregulated by R. solani infection in susceptible cultivar Lemont and was highly expressed in the leaf sheath”
Claim · model-written summary
Overexpression of OsERF65 decreased rice resistance to sheath blight caused by Rhizoctonia solani.
- Taxon
- riceOryza sativae1
- Treatment
- Overexpression of OsERF65e3
- Interactor
- Rhizoctonia solani
Rhizoctonia solanie4pathogen - Gene
- OsERF65e3
- Outcome
- rice resistancee3
- Direction
- decreasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Rice sheath blight (ShB) is a devastating disease”
e3“Overexpression of OsERF65 (OsERF65OE) decreased rice resistance, while the knockout mutant (oserf65) exhibited significantly increased resistance against ShB”
e4“induction of cell death represents a key step during infection by the ShB pathogen Rhizoctonia solani”
Claim · model-written summary
The OsERF65 knockout mutant showed significantly increased resistance to sheath blight caused by Rhizoctonia solani.
- Taxon
- riceOryza sativae1
- Material
- oserf65e3
- Treatment
- knockout mutante3
- Interactor
- Rhizoctonia solaniRhizoctonia solanie4pathogen
- Outcome
- resistance against ShBe3
- Direction
- significantly increasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Rice sheath blight (ShB) is a devastating disease”
e3“Overexpression of OsERF65 (OsERF65OE) decreased rice resistance, while the knockout mutant (oserf65) exhibited significantly increased resistance against ShB”
e4“induction of cell death represents a key step during infection by the ShB pathogen Rhizoctonia solani”
Claim · model-written summary
R. solani infection led OsERF65 to repress peroxidase gene expression in rice.
- Taxon
- riceOryza sativae1
- Treatment
- R. solani infectione5
- Gene
- OsERF65e5
- Outcome
- expression of peroxidase genese5
- Direction
- repressede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Rice sheath blight (ShB) is a devastating disease”
e5“The transcriptome assay revealed that OsERF65 repressed the expression of peroxidase genes after R. solani infection”
Claim · model-written summary
The oserf65 knockout significantly increased antioxidative enzyme activity in rice.
- Taxon
- riceOryza sativae1
- Material
- oserf65 plantse6
- Treatment
- knockout mutante3
- Outcome
- antioxidative enzyme activitye6
- Direction
- significantly increasede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Rice sheath blight (ShB) is a devastating disease”
e3“Overexpression of OsERF65 (OsERF65OE) decreased rice resistance, while the knockout mutant (oserf65) exhibited significantly increased resistance against ShB”
e6“The antioxidative enzyme activity was significantly increased in oserf65 plants but reduced in OsERF65OE plants”
Claim · model-written summary
Overexpression of OsERF65 reduced antioxidative enzyme activity in rice.
- Taxon
- riceOryza sativae1
- Material
- OsERF65OE plantse6
- Treatment
- Overexpression of OsERF65e3
- Outcome
- antioxidative enzyme activitye6
- Direction
- reducede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Rice sheath blight (ShB) is a devastating disease”
e3“Overexpression of OsERF65 (OsERF65OE) decreased rice resistance, while the knockout mutant (oserf65) exhibited significantly increased resistance against ShB”
e6“The antioxidative enzyme activity was significantly increased in oserf65 plants but reduced in OsERF65OE plants”
Claim · model-written summary
The oserf65 knockout reduced hydrogen peroxide content in rice.
- Taxon
- riceOryza sativae1
- Material
- oserf65 plantse7
- Treatment
- knockout mutante3
- Outcome
- hydrogen peroxide contente7
- Direction
- reducede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Rice sheath blight (ShB) is a devastating disease”
e3“Overexpression of OsERF65 (OsERF65OE) decreased rice resistance, while the knockout mutant (oserf65) exhibited significantly increased resistance against ShB”
e7“Consistently, hydrogen peroxide content was apparently reduced in oserf65 plants but accumulated in OsERF65OE plants”
Claim · model-written summary
Overexpression of OsERF65 caused hydrogen peroxide content to accumulate in rice.
- Taxon
- riceOryza sativae1
- Material
- OsERF65OE plantse7
- Treatment
- Overexpression of OsERF65e3
- Outcome
- hydrogen peroxide contente7
- Direction
- accumulatede7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Rice sheath blight (ShB) is a devastating disease”
e3“Overexpression of OsERF65 (OsERF65OE) decreased rice resistance, while the knockout mutant (oserf65) exhibited significantly increased resistance against ShB”
e7“Consistently, hydrogen peroxide content was apparently reduced in oserf65 plants but accumulated in OsERF65OE plants”
Claim · model-written summary
OsERF65 directly binds the GCC box in the promoter regions of four peroxidase genes in rice.
- Taxon
- riceOryza sativae1
- Gene
- OsERF65e7
- Outcome
- GCC box in the promoter regions of four peroxidase genese7
- Qualifier
- directly bounde7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Rice sheath blight (ShB) is a devastating disease”
e7“OsERF65 directly bound to the GCC box in the promoter regions of four peroxidase genes and suppressed their transcription”
Claim · model-written summary
OsERF65 suppresses peroxidase gene transcription, reducing ROS scavenging in rice.
- Taxon
- riceOryza sativae1
- Gene
- OsERF65e2
- Outcome
- transcriptione2
- Qualifier
- reducing the ability to scavenge reactive oxygen species (ROS)e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Rice sheath blight (ShB) is a devastating disease”
e2“OsERF65 directly bound to the GCC box in the promoter regions of four peroxidase genes and suppressed their transcription”
e3“reducing the ability to scavenge reactive oxygen species (ROS)”
Claim · model-written summary
The oserf65 mutant had a slight decrease in plant height in rice.
- Taxon
- riceOryza sativae1
- Material
- oserf65e2
- Treatment
- oserf65 mutante2
- Outcome
- plant heighte2
- Direction
- slight decreasee2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Rice sheath blight (ShB) is a devastating disease”
e2“The oserf65 mutant exhibited a slight decrease in plant height but increased grain yield”
Claim · model-written summary
The oserf65 mutant had increased grain yield in rice.
- Taxon
- riceOryza sativae1
- Material
- oserf65e2
- Treatment
- oserf65 mutante2
- Outcome
- grain yielde2
- Direction
- increasede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Rice sheath blight (ShB) is a devastating disease”
e2“The oserf65 mutant exhibited a slight decrease in plant height but increased grain yield”
The same questions in other papers
Rhizoctonia solani · molecular: expression · Oryza sativa (rice)Positive and negative results
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- positive
- positive
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infection · molecular: expression · Oryza sativa (rice)Positive and negative results
15 papers in the same direction as this paper · 41 in the opposite direction
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53 papers more in this group
See all 57 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.