Abstract
Seed development is critical for plant reproduction and crop yield, with panicle seed-setting rate, grain-filling, and grain weight being key seed characteristics for yield improvement. However, few genes are known to regulate grain filling. Here, we identify two adenosine triphosphate (ATP)-binding cassette (ABC)I-type transporter genes, OsABCI15 and OsABCI16, involved in rice grain-filling. Both genes are highly expressed in developing seeds, and their proteins are localized to the plasma membrane and cytosol. Interestingly, knockout of OsABCI15 and OsABCI16 results in a significant reduction in seed-setting rate, caused predominantly by the severe empty pericarp phenotype, which differs from the previously reported low seed-setting phenotype resulting from failed pollination. Further analysis indicates that OsABCI15 and OsABCI16 participate in ion homeostasis and likely export ions between filial tissues and maternal tissues during grain filling. Importantly, overexpression of OsABCI15 and OsABCI16 enhances the seed-setting rate and grain yield in transgenic plants and decreases ion accumulation in brown rice. Moreover, the OsABCI15/16 orthologues in maize exhibit a similar role in kernel development, as demonstrated by their disruption in transgenic maize. Therefore, our findings reveal the important roles of two ABC transporters in cereal grain filling, highlighting their value in crop yield improvement.
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Claims 11
Claim · model-written summary
OsABCI15 and OsABCI16 were identified as rice grain filling genes.
- Taxon
- rice
Oryza sativae0 - Gene
- OsABCI15 and OsABCI16e0
- Outcome
- involved in rice grain-fillinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we identify two adenosine triphosphate (ATP)-binding cassette (ABC)I-type transporter genes, OsABCI15 and OsABCI16, involved in rice grain-filling”
Claim · model-written summary
Both genes were highly expressed in developing seeds.
- Taxon
- riceOryza sativat0
- Organ / stage
- developing seedse0
- Gene
- OsABCI15 and OsABCI16e1
- Outcome
- highly expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Both genes are highly expressed in developing seeds”
e1“transporter genes, OsABCI15 and OsABCI16, involved in rice grain-filling”
t0“rice”
Claim · model-written summary
Both proteins localized to plasma membrane and cytosol.
- Taxon
- riceOryza sativat0
- Gene
- OsABCI15 and OsABCI16e1
- Outcome
- localized to the plasma membrane and cytosole0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“their proteins are localized to the plasma membrane and cytosol”
e1“transporter genes, OsABCI15 and OsABCI16, involved in rice grain-filling”
t0“rice”
Claim · model-written summary
Knockout significantly reduced seed setting rate.
- Taxon
- riceOryza sativat0
- Treatment
- knockout of OsABCI15 and OsABCI16e0
- Gene
- OsABCI15 and OsABCI16e0
- Outcome
- seed-setting ratee0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“knockout of OsABCI15 and OsABCI16 results in a significant reduction in seed-setting rate”
t0“rice”
Claim · model-written summary
Knockout caused severe empty pericarp phenotype.
- Taxon
- riceOryza sativat0
- Treatment
- knockout of OsABCI15 and OsABCI16e0
- Gene
- OsABCI15 and OsABCI16e0
- Outcome
- severe empty pericarp phenotypee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“knockout of OsABCI15 and OsABCI16 results in a significant reduction in seed-setting rate, caused predominantly by the severe empty pericarp phenotype”
t0“rice”
Claim · model-written summary
The transporters participate in ion homeostasis.
- Taxon
- riceOryza sativat0
- Gene
- OsABCI15 and OsABCI16e0
- Outcome
- ion homeostasise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsABCI15 and OsABCI16 participate in ion homeostasis”
t0“rice”
Claim · model-written summary
The transporters likely export ions between filial and maternal tissues.
- Taxon
- riceOryza sativat0
- Organ / stage
- grain fillinge0
- Gene
- OsABCI15 and OsABCI16e0
- Outcome
- export ions between filial tissues and maternal tissuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsABCI15 and OsABCI16 participate in ion homeostasis and likely export ions between filial tissues and maternal tissues during grain filling”
t0“rice”
Claim · model-written summary
Overexpression enhanced seed-setting rate.
- Taxon
- riceOryza sativat0
- Treatment
- overexpression of OsABCI15 and OsABCI16e0
- Gene
- OsABCI15 and OsABCI16e0
- Outcome
- seed-setting ratee0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of OsABCI15 and OsABCI16 enhances the seed-setting rate and grain yield in transgenic plants”
t0“rice”
Claim · model-written summary
Overexpression enhanced grain yield.
- Taxon
- riceOryza sativat0
- Treatment
- overexpression of OsABCI15 and OsABCI16e0
- Gene
- OsABCI15 and OsABCI16e0
- Outcome
- grain yielde0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of OsABCI15 and OsABCI16 enhances the seed-setting rate and grain yield in transgenic plants”
t0“rice”
Claim · model-written summary
Overexpression decreased ion accumulation in brown rice.
- Taxon
- riceOryza sativae0
- Organ / stage
- brown ricee0
- Treatment
- overexpression of OsABCI15 and OsABCI16e0
- Gene
- OsABCI15 and OsABCI16e0
- Outcome
- ion accumulatione0
- Direction
- decreasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of OsABCI15 and OsABCI16 enhances the seed-setting rate and grain yield in transgenic plants and decreases ion accumulation in brown rice”
Claim · model-written summary
Disruption of maize orthologues showed their role in kernel development.
- Treatment
- their disruption in transgenic maizee0
- Gene
- OsABCI15/16 orthologuese0
- Outcome
- kernel developmente0
Zea maysNot reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the OsABCI15/16 orthologues in maize exhibit a similar role in kernel development, as demonstrated by their disruption in transgenic maize”
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