Abstract
Legumes establish symbiosis with rhizobia, forming nitrogen-fixing nodules. The central role of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in nodule biology has been clearly established. Recently, hydrogen sulfide (H2S) and other reactive sulfur species (RSS) have emerged as novel signaling molecules in animals and plants. A major mechanism by which ROS, RNS, and RSS fulfil their signaling role is the post-translational modification of proteins. To identify possible functions of H2S in nodule development and senescence, we used the tag-switch method to quantify changes in the persulfidation profile of common bean (Phaseolus vulgaris) nodules at different developmental stages. Proteomic analyses indicate that persulfidation plays a regulatory role in plant and bacteroid metabolism and senescence. The effect of a H2S donor on nodule functioning and on several proteins involved in ROS and RNS homeostasis was also investigated. Our results using recombinant proteins and nodulated plants support a crosstalk among H2S, ROS, and RNS, a protective function of persulfidation on redox-sensitive enzymes, and a beneficial effect of H2S on symbiotic nitrogen fixation. We conclude that the general decrease of persulfidation levels observed in plant proteins of aging nodules is one of the mechanisms that disrupt redox homeostasis leading to senescence.
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Claims 6
Claim · model-written summary
Persulfidation plays a regulatory role in plant and bacteroid metabolism and senescence in common bean nodules.
- Taxon
- common bean (Phaseolus vulgaris)
Phaseolus vulgarise1 - Interactor
- rhizobiae2symbiont
- Outcome
- metabolism and senescencee3
- Qualifier
- persulfidatione3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“common bean (Phaseolus vulgaris) nodules”
e2“Legumes establish symbiosis with rhizobia”
e3“Proteomic analyses indicate that persulfidation plays a regulatory role in plant and bacteroid metabolism and senescence”
Claim · model-written summary
Persulfidation levels in plant proteins decreased in aging common bean nodules.
- Taxon
- common bean (Phaseolus vulgaris)Phaseolus vulgarise1
- Organ / stage
- aging nodulese4
- Outcome
- persulfidation levelse4
- Direction
- decreasee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“common bean (Phaseolus vulgaris) nodules”
e4“the general decrease of persulfidation levels observed in plant proteins of aging nodules”
Claim · model-written summary
The decrease of persulfidation in aging nodules is one of the mechanisms that disrupt redox homeostasis and lead to senescence.
- Taxon
- common bean (Phaseolus vulgaris)Phaseolus vulgarise1
- Outcome
- redox homeostasise5
- Qualifier
- general decrease of persulfidation levelse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“common bean (Phaseolus vulgaris) nodules”
e4“the general decrease of persulfidation levels observed in plant proteins of aging nodules”
e5“is one of the mechanisms that disrupt redox homeostasis leading to senescence”
Claim · model-written summary
An H2S donor had a beneficial effect on symbiotic nitrogen fixation in common bean nodules.
- Taxon
- common bean (Phaseolus vulgaris)Phaseolus vulgarise1
- Treatment
- H2S donore7
- Outcome
- symbiotic nitrogen fixatione6
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“common bean (Phaseolus vulgaris) nodules”
e6“a beneficial effect of H2S on symbiotic nitrogen fixation”
e7“The effect of a H2S donor on nodule functioning”
Claim · model-written summary
Recombinant proteins and nodulated plants support a crosstalk among H2S, ROS and RNS in common bean.
- Taxon
- common bean (Phaseolus vulgaris)Phaseolus vulgarise1
- Compound
- H2Se2
- Outcome
- crosstalk among H2S, ROS, and RNSe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“common bean (Phaseolus vulgaris) nodules”
e2“Our results using recombinant proteins and nodulated plants support a crosstalk among H2S, ROS, and RNS”
Claim · model-written summary
Persulfidation has a protective function on redox-sensitive enzymes in common bean nodules.
- Taxon
- common bean (Phaseolus vulgaris)Phaseolus vulgarise1
- Outcome
- protective function of persulfidation on redox-sensitive enzymese2
- Qualifier
- persulfidatione2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“common bean (Phaseolus vulgaris) nodules”
e2“a protective function of persulfidation on redox-sensitive enzymes”