Abstract
Cucumber is one of the important vegetables cultivated in facilities in China. Due to the deterioration of soil quality in facilities and the low nitrogen use efficiency of existing varieties, it is common to pursue high yield through excessive application of nitrogen fertilizer. Improving crop nitrogen use efficiency is one of the important means to reduce nitrogen fertilizer input. Previous studies have shown that NLP (NIN-like protein) transcription factor family play an important role as transcription factors in regulating plant physiological responses to external nitrogen changes. However, there are few reports on NLPs in cucumber. Here we report that CsNLP1 is a potential candidate to improve cucumber nitrogen use ability. CsNLP1 silencing lines by virus-induced gene silencing (VIGS) showed a significant decrease in the plant biomass, chlorophyll content, nitrogen uptake, total nitrogen content, activities of NR, NIR, GDH, GOGAT and GS, and expression levels of genes involved in nitrogen assimilation and signalling under different nitrogen concentrations. In addition, transfer of CsNLP1 into Arabidopsis nlp7-1 mutant found that the phenotype, nitrate (NO3-) content, NR activity, NIR activity, GDH activity, GOGAT activity and GS activity in transgenic lines could be restored to the wild type level after NO3- treatment. Further study found that CsNLP1 could regulate the expression of NO3- responsive genes such as AtNIR1, AtNIA2, AtNRT1.1 and AtGS2 in plants. Overall, our data indicated that CsNLP1 can affect plant growth, and regulate nitrate assimilation by regulating nitrogen-related genes.
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Claims 8
Claim · model-written summary
CsNLP1 silencing decreased plant biomass, chlorophyll content and nitrogen uptake in cucumber.
- Taxon
- cucumber
Cucumis sativust0 - Treatment
- virus-induced gene silencing (VIGS)e0
- Gene
- CsNLP1e0
- Outcome
- plant biomass, chlorophyll content, nitrogen uptakee1
- Direction
- decreasee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in cucumber seedlings”
e0“CsNLP1 silencing lines by virus-induced gene silencing (VIGS)”
e1“showed a significant decrease in the plant biomass, chlorophyll content, nitrogen uptake, total nitrogen content, activities of NR, NIR, GDH, GOGAT and GS, and expression levels of genes involved in nitrogen assimilation and signalling under different nitrogen concentrations”
Claim · model-written summary
CsNLP1 silencing decreased total nitrogen content and the NR and NIR activities in cucumber.
- Taxon
- cucumberCucumis sativust0
- Treatment
- virus-induced gene silencing (VIGS)e0
- Gene
- CsNLP1e0
- Outcome
- total nitrogen content, activities of NR, NIRe1
- Direction
- decreasee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in cucumber seedlings”
e0“CsNLP1 silencing lines by virus-induced gene silencing (VIGS)”
e1“showed a significant decrease in the plant biomass, chlorophyll content, nitrogen uptake, total nitrogen content, activities of NR, NIR, GDH, GOGAT and GS, and expression levels of genes involved in nitrogen assimilation and signalling under different nitrogen concentrations”
Claim · model-written summary
CsNLP1 silencing decreased GDH, GOGAT and GS activities in cucumber.
- Taxon
- cucumberCucumis sativust0
- Treatment
- virus-induced gene silencing (VIGS)e0
- Gene
- CsNLP1e0
- Outcome
- GDH, GOGAT and GSe1
- Direction
- decreasee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in cucumber seedlings”
e0“CsNLP1 silencing lines by virus-induced gene silencing (VIGS)”
e1“showed a significant decrease in the plant biomass, chlorophyll content, nitrogen uptake, total nitrogen content, activities of NR, NIR, GDH, GOGAT and GS, and expression levels of genes involved in nitrogen assimilation and signalling under different nitrogen concentrations”
Claim · model-written summary
CsNLP1 silencing decreased expression of nitrogen assimilation and signalling genes in cucumber.
- Taxon
- cucumberCucumis sativust0
- Treatment
- virus-induced gene silencing (VIGS)e0
- Gene
- CsNLP1e0
- Outcome
- expression levels of genes involved in nitrogen assimilation and signallinge1
- Direction
- decreasee1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“in cucumber seedlings”
e0“CsNLP1 silencing lines by virus-induced gene silencing (VIGS)”
e1“showed a significant decrease in the plant biomass, chlorophyll content, nitrogen uptake, total nitrogen content, activities of NR, NIR, GDH, GOGAT and GS, and expression levels of genes involved in nitrogen assimilation and signalling under different nitrogen concentrations”
Claim · model-written summary
Transgenic Arabidopsis nlp7-1 lines restored phenotype and nitrate content to the wild-type level after NO3- treatment.
- Taxon
- Arabidopsis
Arabidopsise2 - Material
- nlp7-1 mutante2
- Treatment
- NO3- treatmente2
- Gene
- CsNLP1e2
- Outcome
- phenotype, nitrate (NO3-) contente2
- Direction
- restorede2
- Comparator
- wild type levele2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“transfer of CsNLP1 into Arabidopsis nlp7-1 mutant found that the phenotype, nitrate (NO3-) content, NR activity, NIR activity, GDH activity, GOGAT activity and GS activity in transgenic lines could be restored to the wild type level after NO3- treatment”
Claim · model-written summary
Transgenic Arabidopsis nlp7-1 lines restored NR, NIR and GDH activities to the wild-type level after NO3- treatment.
- Taxon
- ArabidopsisArabidopsise2
- Material
- nlp7-1 mutante2
- Treatment
- NO3- treatmente2
- Gene
- CsNLP1e2
- Outcome
- NR activity, NIR activity, GDH activitye2
- Direction
- restorede2
- Comparator
- wild type levele2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“transfer of CsNLP1 into Arabidopsis nlp7-1 mutant found that the phenotype, nitrate (NO3-) content, NR activity, NIR activity, GDH activity, GOGAT activity and GS activity in transgenic lines could be restored to the wild type level after NO3- treatment”
Claim · model-written summary
Transgenic Arabidopsis nlp7-1 lines restored GOGAT and GS activities to the wild-type level after NO3- treatment.
- Taxon
- ArabidopsisArabidopsise2
- Material
- nlp7-1 mutante2
- Treatment
- NO3- treatmente2
- Gene
- CsNLP1e2
- Outcome
- GOGAT activity and GS activitye2
- Direction
- restorede2
- Comparator
- wild type levele2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“transfer of CsNLP1 into Arabidopsis nlp7-1 mutant found that the phenotype, nitrate (NO3-) content, NR activity, NIR activity, GDH activity, GOGAT activity and GS activity in transgenic lines could be restored to the wild type level after NO3- treatment”
Claim · model-written summary
CsNLP1 regulates the expression of nitrate-responsive genes such as AtNIR1, AtNIA2, AtNRT1.1 and AtGS2.
- Taxon
- ArabidopsisArabidopsise2
- Gene
- CsNLP1e3
- Outcome
- expression of NO3- responsive genese3
- Qualifier
- such as AtNIR1, AtNIA2, AtNRT1.1 and AtGS2e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“transfer of CsNLP1 into Arabidopsis nlp7-1 mutant”
e3“Further study found that CsNLP1 could regulate the expression of NO3- responsive genes such as AtNIR1, AtNIA2, AtNRT1.1 and AtGS2 in plants”
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